Tangential Flow Filtration - PPT.pptx

Tangential Flow Filtration a brief discussion about the normal flow and TFF

1. Tangential Flow Filtration

2. What is Filtration? Filtration separates particles based on size difference through the pores of a membrane known as a filter . The fluid or particle that is smaller than the size of the pores in the filter passes through a filter ( filtrate or permeate ) while the bigger particles will be trapped by the filter ( retentate ).

3. Types of FILTRATION Filtration can be performed in one of two operational modes Normal Flow Filtration Tangential Flow Filtration

4. Normal flow filtration Dead End Filtration Direct Flow Filtration (DFF) Feed flows in perpendicular to the to the filter membrane.

5. Tangential flow filtration Cross Flow Filtration Feed flows in Parallel to the filter membrane.

6. Tangential flow filtration

7. WHAT DOES A TFF DO? Purification (separates components by size, removes impurities) Concentration (reduces volume, de-waters) Buffer Exchange or diafiltration (formulation)

8. TFF IN A PROCESS

9. APPLICATIONS OF Tangential flow filtration Separation of large and small biomolecules, clarification and/or removal of contaminants. Purify and/or recover plasmid DNA from cell culture or cDNA from blood. Cell harvesting (bacteria, yeast, mammalian cells.) Reduce Bioburden and/or clean up water, solutions, buffers, and media (remove and/or reduce endotoxin load.) Recover and or remove virus particles and/or vaccines.

10. Various filters Different filters based on their pore sizes: macro-, micro-, ultra-, and nano -filters

11. Thank you