Tools of biotechnology

this presentation deals with the basic undestanding of biotechnological tools

1. BIOTECHBIOTECH
ASSIGNMENTASSIGNMENT
submitted by:submitted by:sunnysunny

2. Recombinant dna technologyRecombinant dna technology
A technology which involves the A technology which involves the
combining or rejoining of dna combining or rejoining of dna
fragment from two different fragment from two different
organismsorganisms

3. Basics steps in rDNA technologyBasics steps in rDNA technology

1.1.isolation of dna fragment containing a isolation of dna fragment containing a
gene of interest.gene of interest.

2.Generation of a recombinant dna 2.Generation of a recombinant dna
molecule by insertion of dna molecule by insertion of dna
fragment into a carrier dna molecule.fragment into a carrier dna molecule.

3.3.transfer of the rDNA into an e.coli host transfer of the rDNA into an e.coli host
cellcell

4.selection of only those host cells 4.selection of only those host cells
carrying the rDNA and allowing them to carrying the rDNA and allowing them to
multiply multiply

4. Tools of Recombinant DNA techTools of Recombinant DNA tech

1.restriction enzymes1.restriction enzymes

2.vectors 2.vectors

3.host cell3.host cell

5. RESTRICTION ENZYMERESTRICTION ENZYME

The molecular scissorThe molecular scissor

6. These are the protein molecules also called as the These are the protein molecules also called as the
molecular scissor becouse they helpto cut the dna at molecular scissor becouse they helpto cut the dna at
specific sitesspecific sites

properties:-properties:-
1.A restriction enzyme that 1.A restriction enzyme that
selectively recognise a specific DNA selectively recognise a specific DNA
sequence and digest any DNA sequence and digest any DNA
fragment containing that sequence.fragment containing that sequence.
2.A modification enzyme that 2.A modification enzyme that
adds a methyle grup to one or two adds a methyle grup to one or two
bases with in the sequence bases with in the sequence
recognised by the enzyme.recognised by the enzyme.

7. Some restriction enzymesSome restriction enzymes

8. vectorsvectors

Vehicles for cloningVehicles for cloning

9. plasmidsplasmids

Plasmids are extracromosomal,self-Plasmids are extracromosomal,self-
replicate,usually circular,double replicate,usually circular,double
stranded DNA molecule found stranded DNA molecule found
naturally in many bacteria and in naturally in many bacteria and in
some yeasts.some yeasts.

10. Some plasmidsSome plasmids
1.PBR322 2.puc 19 1.PBR322 2.puc 19

11. Host cellsHost cells

13. Thank for giving me this Thank for giving me this
opportunityopportunity
Have a nice dayHave a nice day