Translational control

Translational Control

1. BHARATHIDASAN UNIVERSITY TIRUCHIRAPPALLI-620 024 DEPARTMENT Of MARINE BIOTECHNOLOGY SEMINAR ON MOLECULAR BIOLOGY AND MICROBIAL GENETICS By Sukumar Ranjith M.Sc.,Marine Biotechnology

2. Translational control

3. Translational control Translational control plays an essential role in the regulation of gene expression . Protein synthesis is an indispensable process in the pathway of gene expression, and is a key component in its control. Regulation of translation plays a prominent role in most processes in the cell. The synthesis rate of a protein in general is proportional to the concentration and translational efficiency of its mRNA.

4. Translational control Ribosome binding , Codon usage , Antisense RNA

5. Ribosome binding Translational controlled by the ribosome binding is defined as the ribosomal protein binding to the mRNA leads to a selective reduction in translation. For example, Hox mRNA translation

6. Codon usage Codon frequencies and tRNA abundances tRNAs translate codons into amino acids during protein synthesis. Every organism has multiple tRNA species that read the codons for the same amino acid ( tRNA isoacceptors ). The codons also plays a vital role in tranlational control.

7. Codon usage

8. Antisense RNA Antisense technology involves the production of complementary nucleic acid molecules against the mRNA molecule transcribed from the DNA in order to stop the translation into protein. These complementary molecules can be synthetically produced and delivered inside the cell to block the expression of diseased protein. It can be a short length of either RNA or DNA which commonly termed as Antisense oligonucleotides (AON)

9. Cont ’ This phenomenon is still not well understood but the current hypothesis about this is following-: blocking RNA splicing,  accelerate the degradation process of the RNA and it also prevents the introns from splicing  preventing the migration of mRNA from nucleolus to cytoplasm  stopping the translation of diseased protein, and  If complementary DNA molecule is used there may be a formation of triplex in DNA template.

10. Anti sense RNA

11. Th an k y ou !!!