Types of Bioreactor

Types of Bioreactor

1. DIFFERENT TYPES OF BIOREACTER Amjad Khan Afridi

2. Amjad Khan Afridi DIFFERENT TYPES OF BIOREACTER

3. Mixing the media & others contents Separation of Media and Microbes Maintain Temperature Remove gas and bubbles Sparger

4. Mixing the media & others contents Separation of Media and Microbes Ph Temperature Aeration Maintain Temperature

5. Stirred Tank Bioreactor In this bioreactor air is dispersed by mechanical agitation (impellers) . Temperature, pH, amount of dissolved oxygen and nutrient concentration can be controlled better with this reactor as compared to other reactors. Baffle and rotating stirrer is attached either on the top or at the bottom. Impeller speed is reduced to 50-150 rpm

6. An agitation system An oxygen delivery system A foam control system A temperature control system A pH control system Sampling ports A cleaning and sterilization system Basic Features

7. A i r lif t B i o r e ac t o r Compressed air is used for aeration and mixing of the contents. This is one of the most suitable bioreactor types for cultivation of plant cells on a large scale. Properly mixing of Oxygen and nutrients inside the vacume. In ALRs, the culture medium is kept mixed and gassed by  sparging air  at the bottom of the  bioreactor  equipped with either an inner draught tube or an external loop .   This generates a vertically circulating flow that improves oxygen transfer, and levels shear forces.

8. A i r lif t B i o r e ac t o r Merits: It provides reasonable mixing and oxygen transfer at low shear. As it has no moving parts, it has less contamination problem. Operating cost is lower than stirred tank reactor. Demerit: Develop dead zones, due to insufficient mixing at high cell density

9. Bubble C olumn B ioreactor This is one of the simplest bioreactor. It consists of a cylindrical vessel aerated at the bottom. Used for production of metabolites from yeasts Also used for wastewater treatment. The air is pumped through a plate located at the base of the bioreactor The plates has many openings through which air or gas is passed in the form of bubbles into the liquid medium contained in the bioreactor Uniform maxing of the contents Lack of impellers

10. Advantages Have high volumetric (Volume) productivity and excellent heat management. Better utilization of the plate area and flow distribution. Self regulating. Disadvantages Less efficient than other bioreactors Does not have draft tube Higher catalyst consumption than the fixed bed Higher installation cost and difficult to design

11. Fluidized Bed Bioreactors Fluidized bed bioreactor is comparable to bubble column bioreactor except the top position is expanded to reduce the velocity of the fluid. The design of the fluidized bioreactors is such that the solids are retained in the reactor while the liquid flows out. These bioreactors are suitable for use to carry out reactions involving fluid suspended biocatalysts such as immobilized enzymes, immobilized cells, and microbial flocs.

12. Advantages: Uniform particle mixing. Uniform t empe r a tu r e gradient. Ability to operate reactor in continuous state. Dis-advantages: Increased reactor vessel size. Pumping r equi r eme n t. Pressure drop.

13. 16th June, 2021