Types of explant and culture

Plant Tissue Culture Techniques

1. PREPARATION AND CHOICE OF EXPLANT FOR PLANT TISSUE CULTURE AND TECHNIQUES S. No. CONTENT 1 Types of Culture 2 Types of Explant 3 Sterilization 4 Sterilization Protocols 5 Cell Phases 6 Sub- culturing

2. TYPES OF CULTURE

3. PLANT PARTS USED IN TISSUE CULTURE

4. TYPES OF EXPLANT- Meristem Culture Meristem Culture comprised of meristematic regions like SAM (Shoot Apical Meristem), RAM (Root Apical Meristem) and auxiliary shoots.

5. SEED/ OVULE CULTURE Here the explant is fertilized ovule of those plants that produce low level of viable seeds or whose seeds are minutes as well as non albuminous type.

6. EMBRYO CULTURE

7. CALLUS CULTURE Callus is an unorganized mass of cell that contain only parenchymatous cells

8. HAPLOID CULTURE Haploid can be generated from pollen, anther and unfertilized ovum and majorly used for pure line production.

9. ORGAN CULTURE

10. PROTOPLAST CULTURE

11. STERILIZATION OF EXPANTS

12. STERILIZATION PROTOCOLS Protocols of sterilization are specific for different explants. Harsh explants requires long duration as well as high concentration of chemicals While soft tissues like embryos, anther and ovules require particular types of chemicals. Sterilization process are followed in outer area as well as in laminar air flow hood. Increased concentration of chemicals will affect the viability of explant.

13. PHASES OF CELLS Growth of cells

14. EXPONENTIAL GROWTH

15. When the cells in culture are reaching at stationary state it should be sub-cultured. Sub-culturing may be in similar or dissimilar type of media with respect to the requirements.