UG IV SEMESTER ZOOLOGY PRACTICAL MANUAL

It covers cell biology,molecular biology,genetics and Developmental biology topics

1. VAM

YLLABUS

AR A

UNDER GRADUATE COURSES (UNDER CBCS 2020 - 2021 ONWARDS)
B.Sc. Zoology HI Year
Semester - IV
CELL BIOLOGY, GENETICS & DEVELOPMENTAL BIOLOGY PRACTICAL

LevroLocy
1. Preparation and Identification of slides of titotic divisions with onlon root
ts.
2. Preparation and Identification of diferent stages of Meiosis in Grasshopper
Testes.

3. . Identification and study of the following slides.
{) Different stages of Mitosis and Meiosis.
(i) Lamp Brush and Polytene chromosomes.

TLGENETICS
1: Problems on Genetics - Mendelian inheritance, Linkage and Crossing over,
Sex inked inheritance. >
‘I, EMBRYOLOGY
1. Study of TS. of Testis and Ovary of a mammal.
2. Study of different stages of cleavages (2,4,8,16 cell stages); Morula,
Blastula.
3. Study of chick embryos of 18 hours, 24 hours, 33 hours and 48 hours of
incubation. E
Laboratory Record work shall be submitted at the time of practical
examination.
V. An Album“ containing photographs, cut outs, with appropriate write-up
about Genetics and Embryology.
computer aided techniques should be adopted as per UGC guide Ines:

2. ¡AAA
ONTENTS... Bin
y
Eee]

(2) Set Une Trug Crossing rer Gene Mapping. 2

[EMBRYOLOGY]

1. Study of, of ead Oar fa Hamm, El

2, Saye teca sages done (2496 al tape); Mora,

3. 7 __

Es

imroduton San eign ne cost ais pd to st re
ses mt amsn tsar cal of reshapes era esr
tested ee the cone sip pen over he pre sed tse be al HPP Or

ee}

am
0

4. ——— ACES]

3. PROPHASE ens bye pan l
I M erstes a cree quan of mage conter,
A Grama re wa resin up Ino deta ube 8 ng cal ted he
rampas.
3. Core become sor ue o tant, candensaton an enero,
4. Lan me pt e
‘dled name

Tee cent apart nd mp oar poes tating Ui fan a mich
nde,
3 METAPHASE sents by ne presence of
1. Tol csappsance ercer montan.
2. formen faster and te in

3. Formeln ol mear mentrare ad rc tra
rn

4. Aoperance a dep conti a cate of the el nating te erties

ES)

5. (3.(11) MEIOSIS (REDUCTION DIVISION) = Y
(HETEROTYPIC DIVISION)

1. en carn rm mote ct resungin te formation EEE
2. Deugner ce my beau min pematgeps rural sn gene.
3, Remedi cal rocas epa cs
4. Heel mamiangin point logan ro gels
5. New carci are formed In he roger eto rig over In oh
DUO or PROPMASE-L — GS
Formato esti romane ch re thn trend er roms

mt to oppose slim meat sp.
arco rana oF PROPHASE +

. Sap Ping of mat nd potent oras) t fem

‘comple i formas of proteins to fate gara
(Gy PACITENE Or PRODMASE=1
1. Gromosames at gee frm evel nd un
ma astres
2. unbercondansten and nat ma
ke nn mis von in ie
si crm pot cn en eas ta
4 The ponts rece ae ve cms

Sewage el remata cena and echnge

6. emp menses cit
: ss reaps cnr Sn MERE
se ran ts mt a ns ate ade
| essa
oti von) o ras, a MME
ne eyed deca art na Dr or
=
momse-n:
À renard rt can

2. Coromemenor visibles eprlzaton aná yan.
ve te cenremere ef the vemosamé ia at ine

sc rom theatre and atch win cra

te formation of mt sp

poe fringe pre mt son
e formation and separation of davahter

arenosas — À
ont rames at ing to the aster cose finira onde,

3. ngatin of nee hromasomal feat e easy eperaten and moveent of
3. pd cvoresomes wt exenonges gen mater per va spe

Does
>. one vies he al sr nthe oration o o auge opio

Lame bah cvemosoms an ey be separated ln

wen]

cm

7. — Faman
_[eenerics

PROBLEMS ON GENETICS -MENDELIAN
INHERITANCE, LINKAGE AND CROSSING
OVER, SEX LINKED INHERITANCE.

1, PROBLEMS BASED ON MENDELIAN INHERITANCE]

8. [PRACTICAS]

eaten) 1 oma sr) —> ng amt
a
On
onl

9.6 Winn rue-rending alstem pa planes ae crossed wth trve-breedng
short stem pes plants, what isthe phenotype ature ofthe FL and 2 generation
character

9. ma

y [Pracricnis]

900%

[S888

BE when do yout php ons atom
of twa organisms heterozygous for a single trait? a

Phenotype rato of 1
ee

ere win dan oracion

(1) ent mcm carr

10. 1 [pm

tn pte et sg ewe a ug

11. ones PRESS)

{2.11 Pr the phenotype ratio 9:32 forte pin oa serve
‘ser rro. a mi

=

rs

"Dentd, green phenatyp Ass pat would be rec er Bt.
ot nas ted re sede plans. 5 (1/16),

12. a na
Alla of parent 2: Ter ety an post comia of ala We Somat
Etes
e
T
@

ms Romus ner the see Dear seed coer al.

enc velo sede plane

an

oT"

Phenbype of orig: Each of he greys ef the ong camspandı to à
rent shen

Ss are smoat, yew es

Sy are soa, gun ses

moe devas, ve sed

En]

9.14 m0 bricos, 4300 * nat, mat
tino tering witb baryon fer ht racer

setter pn oe AN

Both parents rece 20% echt AB, 0,00 an ad.

= olen)

Gi

13. 4 STUDY OF LINKAGE THROUGH
CROSSING OVER & GENE MAPPING

enti Unkage Unies genes sos toga on ronosame, mai am
(nr toner (e), Genes on sgaraa Oronetmes ae hve eked
i gene on rames re rs. Cane tat ae fer moy rom
ere yt be spre ing a proces cles Palau cin

14. San re eo

15. Is conducted between two 1-hyèrié pes plants fer sphere
at tt wt re elit

16. PRASTICALS]

Im Mande! experiments te gen for tal (7) plants was incompletely
ont oi genase ls bars of re

Er

on etage tenn mt tr op ta

(Me preci segon ptm nt F2 generan som
nee (reg) an ma an romo

— i)

17. Pigs TS Tete

8. Atado cams cepas um sce y aa at.

2. Garpsteideums be peon mass of lacie tee ores atthe poetes
ener, sang presen

18. [1 | zoo.0ey - sense | =

STUDY OF DIFFERENT STAGES OF CLEAVAGES
(2.448,16 CELL STAGES), MORULA, BLASTULA

Be: bts

Fig: Soga flema
Un Bo (call Sg) ee Sage) Ce Sage

A

Ar

19. _$—_—_— [pracricals]
(© Blastala of ia of Fred] STUDY OF CHICK EMBRYOS OF 18 HOURS,
une snl ep, ar atlas 24 HOURS, 33 HOURS AND 48 HOURS
OF INCUBATION

& (2)18H.chick Embryo)

Fie: Beale

(Blend (Frl Seton of Bar showing Be

20. transform io ona
and pr YE

3. (b)24H, Chick Embryo

1. Ferien interna and derdopmen ent! oF eerie HO

rode by the mater Be rig

Pig: 24H Chick Ebro

12 beep noth been the hea and te under ylk materalis soez
octet.
GE —

22. FFs. [zootogy - SEMESTERAIV

13. Yolk sac and Allantols are formed from the hindgut region
embryo,

‘at the posterior end of the

n. First gill pouch fu
14. Thre pars of il pouches are formed rom the pharyngen regia dors ses
with ectodermal invagination to form the first gil lt 1.8. 5

Fig : 48 H Chick Embryo