Vaccine Production

Introduction
Vaccines do?
History
Types
Stages
Conclusion

1. VACCINE PRODUCTION PRESENTED BY- NILAMBAR KHURA M.SC IN ZOOLOGY 18PZOL12 GOVT.AUTONOMOUS COLLEGE,ROURKELA

2. CONTENTS Introduction History What Vaccines do? Types of Vaccine Production of Vaccine Significance Reference

3. INTRODUCTION Vaccine may be defined as the agent which enhance the active immunity of the body. They give us a healthy defense. The vaccine used to treat for a particular infection is called “ vaccination ”.

4. HISTORY The word ‘Vaccine” originates from the Latin word vaccinus . Edward Jenner demonstrated in 1798 could prevent smallpox in humans. Today the term “ Vaccine ” applies to all biological preparations, produced from living organisms, that enhance immunity against disease. Edward Jenner 1749-1823

5. What vaccines do? When inactivated or weakened disease- causing microorganisms enter the body, they initiate an immune response. This response mimics the body’s natural response to infection. These antigens triggers the production of Antibodies by the immune system. Antibodies bind to corresponding antigens and induce their destruction by other immune cells.

6. Source –www.sonofi.co.in TYPES OF VACCINES

7. Production of vaccine Vaccines are produced in large scale as they need to be administrated to large populations of children and adults to be effective as a public health tool. For the production of vaccines has several stages through which vaccine produced.

8. Stages of vaccine production Generation /Isolation of Antigen Purification Inactivation Formulation Filling Batch release Packing and shipping

9. Stages of vaccine production Sourse-www.snofi.com

10. 01.Generation/Isolation of antigen Viruses are grown on primary cells such as cells from chicken embryos or using fertilized eggs (e. g. influenza vaccine)or cell lines that reproduce repeatedly( e.g. hepatitis A). Bacteria are grown in bioreactors which are devices that use a particular growth medium that optimises the production of the antigens Recombinant proteins derived from the pathogen can be generated either in yeast, bacteria or cell cultures. Production starts by growing viruses bacteria in the lab a wide range of program meter manages like – Temperatures PH Oxygen Rate Sterility Homogeneity

11. 02.Purification The microorganisms are extracted from the environment through a process called purification which are ruminates any traces in culture media.

12. 03.Inactivation Then inactivate the virus or Bacteria that ruminates to ability to cause disease. We retain its ability to leisure precautionary immune response from the body. That activated virus or bacteria is called a valence.

13. 04.Formulation Once the antigen is developed the vaccine is formulated by adding adjuvant, stabilizers, and preservatives. The role of the adjuvant is to enhance the immune response of the antigen. The stabilizers increase the storage life, and preservatives use of allow multi dose vials.

14. 05.Filling When its times for the vaccine to be administrated it would be reconstituted by combining the powder with diluents. The vaccine is liquid or freeze dried form and diluents is needed and then filled into seeds or syringes . Each phase undergoes visual inspection. Quality containers are scrutinized by human eye and sterile in digital surveillance technology.

15. 06.Batch release Samples are taken from each batch. After vaccines are successfully passed both sides of test distribution began. No exception if a batch is not matched with quality criteria will be destroyed.

16. 07.Packing and shipping For the packing and shipping vaccine must be stored in 2 to 8 C . Temperatures tracking device is included in each shipment .

17. Significance of vaccine production Makes good economic sense, and meets the need to care for the weakest members of societies. Reducing global child mortality. Global life saving immunization against deadly dieses like meales , H ib and rotavirus.

18. REFERENCES http://www.biotec.or.th/biosafety/download/biotec%20seminar%202.pdf http://www.cfsciences.com/pdf?3.%20vaccine%20production%20ver7.pdf www.hsc.gov.sg www.sonofi.com www.sciencedirect.com w ww.shareyourarticles.com 7. Life sciences: Fundamental and practice[5 th edition]

19. THANK YOU