VEKTOR PLASMID, CHARACTERISTICS PLASMID, AND FUNGSI

vektor plasmid

1. VEKTOR
PLASMID
Novi Febrianti
Prodi Pendidikan Biologi
UAD

2. PLASMID
What is plasmid?
Structure
Characteristics
Function & Application
Function in Genetic Engeneering

3. What is plasmid?
•They are extra chromosomal DNA fragments present in the cell
•They are double stranded structures (exceptions are the linear
plasmids in bacteria Streptomyces spp and Borrelia spp)
•They can replicate independently
•The absence of a plasmid in the cell does not affect cell
functioning, but the presence of a plasmid in the cell is usually
beneficial.

4. Plasmids
Small circular DNA molecules that replicate independently of the
host chromosomes

5. Plasmid Characteristics
•Ukuran plasmid bervariasi 2 kb - 200 kb.
•Plasmid berukuran kecil umumnya berjumlah sedikit,
sedangkan plasmid berukuran besar jumlahnya lebih banyak.
•Kebanyakan plasmid mengandung gen TRA , yaitu gen yang
penting untuk mentransfer plasmid dari satu sel ke sel lain

6. Structure of Plasmids
Unsur penting pada plasmid:
•Origin of replication (OR) – Tempat
dimana proses replikasi dimulai
•Selectable marker site – Daerah dimana
terdapat gen yang resisten terhadap
antibiotic (berguna saat akan identifikasi dan
seleksi bakteria yang mengandung plasmid)
•Promoter region – Daerah tempat
mengawali proses transkripsi
•Primer binding site – urutan DNA pendek
untai tunggal yang bermanfaat dalam
amplifikasi (penggandaan) dan sekuensing
DNA
•Multiple cloning sites/Restriction sites
– Mengandung berbagai sekuen tempat
enzim restriksi terikat dan memotong
struktur rantai

7. Functions and Applications
of Plasmids

•Dapat digunakan sebagai vektor untuk menyisipkan gen
tertentu dan mentransfernya ke sel lain. Sering digunakan untuk
penghantaran obat dan produksi hormon pada sel lain
•Mengandung gen yang resisten terhadap antibiotik
•Plasmid degradative dapat mendegradasi bahan kimia industri
yang membahayakan lingkungan
•Sumber penting untuk transfer gen secara horizontal (antar
sel).

8. Plasmid function in Recombinant DNA Technology

9. Plasmids used in molecular biology have been constructed in the lab
Molecular cloning




Enzymes are used to insert desired
pieces of foreign DNA into plasmids







Bacterial cells are transformed with
the plasmids. Copies of the plasmids
are purified from bacteria.

10. Shuttle vectors have origins of replication and selectable markers
for propagation in both bacteria and yeast
We are using plasmids that have been termed shuttle vectors,
because they can be propagated in either bacteria or yeast
Plasmids are propagated in
bacteria, which grow quickly
and maintain multiple copies
of the plasmids
non-pathogenic strain
of Escherichia coli
Saccharomyces cerevisiae
deletion strains
Plasmid-encoded genes
are expressed in yeast,
and phenotypes are
analyzed

11. Referensi
•Brown, T.A., 1991, Pengantar Kloning Gena, Yayasan Essentica Media
•https://microbenotes.com/plasmids/