WATER TREATMENT USING GUAVA LEAVE AS ANTIMICROBIALpptx

WATER TREATMENT USING GUAVA LEAVE AS ANTIMICROBIAL

1. NELSON MANDELA AFRICAN INSTITUTION OF SCIENCE AND TECHNOLOGY (NMAIST) TITLE: Assessing Extracts of Psidium guajava L. for Treating  Vibrio cholerae -Contaminated Water in Northern Tanzania NAME OF STUDENT: Noela Olivia Enock REGISTRATION NO: M96/T23

2. PRESENTATION OUTLINE Introduction Problem Statement & Justification Objectives of the Study Methodology

3. Introduction Waterborne diseases remain significant public health challenges, particularly in rural areas where access to clean water and sanitation facilities is often limited. Cholera, caused by the bacterium Vibrio cholerae. (World Health Organization [WHO], 2022). In rural areas infrastructure for water treatment is still a problem which cause the risk of cholera and other waterborne due poor sanitation and contamination.

4. Cont …Introduction Factors like environmental and socio-economic conditions. For instance, rural communities may rely on surface water sources that are more susceptible to contamination by pathogens from human and animal waste (Barrell, 2019). S easonal variations and extreme weather events, such as floods, can cause water contamination issues and increase the incidence of disease outbreaks ([CDC], 2021).

5. Problem Statement & Justification S hallow wells are primary source of drinking water in rural area. The quality of water from these wells may posing significant health risks to the population Application of methods for purifying water such as boiling, filtration, chlorination, solar densification, UV light treatment, activated carbon filtration not practice in rural areas Cannot afford these methods since they are expensive and not accessible therefore they require an alternative, simple approach and cost-effective solutions for water treatment.

6. Cont … Problem Statement & Justification Traditionally knowledge, guava ( Psidium guajava L) leaves as a natural medicinal properties can be affordable and accessible in rural areas as a solution for water treatment. Due to its potential there is limited scientific research on its effectiveness in treating Vibrio cholerae - contaminated water. Therefore I introduce a new study in order to assess the antimicrobial efficacy of Psidium guajava L. leaves against Vibrio cholerae in contaminated water sources in Northern Tanzania.

7. Objectives & Expected output Objectives General Objective: To determine capacity of Psidium guajava L. extracts for treatment of Vibrio cholerae from contaminated waters. Sp e cific Objectives To assess effectiveness of Psidium guajava L. leaves against  Vibrio cholerae in contaminated water To determine the optimal dosage for Psidium guajava L. leaves of contaminated water To evaluate changes of water quality after treatment with Psidium guajava L. leaves Expected Output The study will be able to demonstrate that Psidium guajava L. leaves has effect in significantly reducing or eliminating Vibrio cholerae in contaminated water The optimal dosage of the extract is expected to be identified The water quality is expected to improve or remain within the standard for consumption

8. Objectives of the Study General Objective: To determine capacity of Psidium guajava L . extracts for treatment of V ibrio cholerae from contaminated waters Specific Objectives To assess effectiveness of Psidium guajava L. leaves against  Vibrio cholerae in contaminated water To determine the optimal dosage for Psidium guajava L. leaves of contaminated water To evaluate changes in of water quality after treatment with Psidium guajava L. leaves

9. Research Question How effective are Psidium guajava L. leaves in reducing Vibrio cholerae levels in contaminated water? What is the optimal dosage for treatment while maintaining water quality?

10. Methodology Objective: 1 Procedures Equipment & Materials To assess effectiveness of Psidium guajava L . leaves against  Vibrio cholerae in contaminated water -Identifying potential plants with antimicrobial properties. -Collecting plant samples from Northern Tanzania. -Grinding and extracting using distilled water -Using agar well diffusion to test extracts against Vibrio cholerae . -Determining the minimum inhibitory concentration (MIC) using a broth dilution method -Plant collection tools - Mechanical grinder - Soxhlet apparatus - Rotary evaporator - Mueller-Hinton agar plates - Incubator - Micropipettes

11. Methodology Objective. 2 Procedures Equipment & Materials To determine the optimal dosage for Psidium guajava L. leaves of contaminated water -Creating water samples contaminated with Vibrio cholerae. -Testing various concentrations of plant extracts in water. -Measuring the reduction in bacterial count post-treatment. -Identifying the dosage that maximizes bacterial reduction with minimal extract. -Contaminated water samples - Micropipettes - Incubator - Colony counter - Sterile containers

12. Methodology Objective.3 Procedures Equipment & Materials To evaluate changes of water quality after treatment with Psidium guajava L. leaves -Identifying key parameters to monitor. - Measure water quality before treatment. - Applying optimal dosage of plant extracts. - Measure changes in water quality parameters and assess toxicity. -Multiparameter - GC-MS/LC-MS/MS

13. References