Whole genome sequence.

Whole genome sequence it's one the dna sequencing method. In this slide is contain method of sequence and advantages.

1. WHOLE GENOME SEQUENCING SUBMITTED BY: K. JAYALAKSHMI I M.SC., BIOTECHNOLOGY

2. DEFINITION Whole genome sequencing (WGS), also known as full genome sequencing or entire genome sequencing , is the process of determining the entirely or nearely, of the DNA sequence of an organisms genome at a single time. Developed by j. craig venter and H. smith in 1995. It was first used in sequencing the genome of H. influenzae and M. genitalium.

3. WHOLE GENOME SEQUENCING METHODS (WGS) Shot gun sequencing Massively parallel signature sequencing Pyro sequencing Illumina seqencing DNA nanoball sequencing Heliscope single molecule sequencing Single molecule real time sequencing (SMRP) Nanopore DNA sequencing Solid sequencing

4. WHOLE GEENOME SHOTGUN SEQUENCING To sequence all genome of a particular organisms. 1) find sequence 2 ) find their position in e ntire genome Collect and isolate the DNA or genome. Break into small manageable pieces. Copy each piece many times. Read the DNA sequence. Assemble the data into a genome. STEPS

5. WGS Different technique are used to cut DNA into particular size pieces. ( fragments are electrophoreses through gel to find the size of fragments.) Ligation of fragment into a plasmid. Insert plasmid into bacteria e.g. E.coli. Micro titer plate containing E.coli is heated to 95 degree celsius. Plasmid are released. PCR reaction (amplify the plasmid) Desired sequence amplification DNA attracted toward the carboxyl coated magnetic bead.

6. Sanger sequencing Assembly i) multiple read of the same genome is made. ii) reassembles by matching overlaps.

7. WGO

8. ADVANTAGE To find coding non and non coding regions. Personalized drugs. Disease susceptibility prediction. Specie comparison and evolutionary sudies .

9. DISADVANTAGE Most physicians are not trained in how to interpret genomic DNA data. An individuals genome may contain information that they don’t want to know. For example, a patient has genome sequencing performed to determined the most effective treatment plan for high cholesterol.