Work Plan Strategies for assessing the adverse effects of pesticide use and their accumulation in soil, food grains, and vegetables.pptx

WORKPLAN: Strategies for assessing the adverse effects of pesticide use and their accumulation in soil, food grains, and vegetables

1. WORKPLAN: Strategies for assessing the adverse effects of pesticide use and their accumulation in soil, food grains, and vegetables. PLANT PATHOLOGY-MICROBIOLOGY PLANT BIOTECHNOLOGY NANOTECHNOLOGY INTERDESIPLINARY APPROACHES PROPOSED ACTIVITIES Profiling of selected crops’ edible leaves/seeds for protein content, micronutrient density (Fe, Zn, Cu, Mn, and B etc. Determining pesticide residues in the edible leaves and seeds using HPLC-MS/MS Identification of pesticide residues accumulating beyond threshold level in edible part Defining pesticide application to enhance yield and reduce pesticide residues. Comparative estimation of micronutrients and pesticide in edible leaves/seed Raising selected genotypes ( in vitro / in vivo conditions) under application of different dosages of pesticides and herbicides Phenotyping of the genotypes raised under application of different dosages of pesticides Genome-wide association mapping of candidate loci and gene(s) responsible for uptake and transport of micronutrients and pesticide in to edible leaves and sink SNP profiling of experimental genotypes Population Structure Analysis GWAS Analysis and identification of Candidate Gene Genomic Prediction Analysis Identification of loci and candidate gene(s) regulating uptake and transport of nutrients and pesticide in to sink using genome-wide association study (GWAS) Examining effect of pesticide application on soil physicochemical properties (texture and pH) Assessment of microbial diversity and abundance in pesticide treated soils Assessment of microbial parameters including nitrogen, CO 2 utilization, colony units, structure and composition of community, microbial biomass, microbial genetic structure, soil-microbe relationship and microbial thermodynamics Determining pesticide induced oxidative stress and DNA Damage in the plant growth promoting soil micro-organism(s) Collection of plant growth promoting micro-organisms from the pesticide treated and untreated soil Estimation of Reactive oxygen species (ROS), Malondialdehyde (MDA), superoxide dismutase (SOD), catalase (CAT), and glutathione s-transferase (GST) etc., Metabolomics and compound analyses via High-Resolution UHPLC Determining sub-lethal effects of selected pesticides on the DNA of soil organisms using metagenomics and metabolomics approach Selection of aptasensors specific to pesticide Synthesis of Gold nanoparticles and its fictionalization Conjugation of aptasensors to gold nanoparticles Development of gold nanoparticle conjugated aptamer detection kit for pesticides Development of DIY kits for rapid detection of targeted pesticide residue(s) in plant 11-20 M 13-24 M 11-24 M 25-36 M 0-10 M Assessment of pesticide exposure and its effects on soil microbial populations Identification of absorbed pesticide residues and perception on how they relate to serious health risks APPROACHES AGRONOMY-SOIL SCIENCE-BIOCHEMESTRY POOLED ANALYSIS Pravin V. Jadhav (PhD), Biotechnology Cenre , Dr. PDKV., Akola