xanthoprotic millons test.pptx

physio

1. Motto Vision; The Dream/Tomorrow To impart evidence based research oriented medical education To provide best possible patient care To inculcate the values of mutual respect and ethical practice of medicine

2. MSK-II Module Skill Lab Xanthoproteic Test Millon Nasse’s Test Biochemistry Dept

3. Learning Objectives At the end of this session students will be able to learn Purpose and chemical reagents of xanthoproetic and millon nasses’s test To perform chemical test to detect the presence of aromatic amino acids and tyrosine Interpret the findings obtained

4. Professor Umar Model of Integrated Lecture 5

5. Horizontal integration (Anatomy and Physiology)

6. Core concept

7. EXPERIMENT 2ml original solution (egg white) + 1ml concentrated HNO3 +mix till white ppt are formed + heat for 1min till ppt turn yellow + cool + add 1ml strong ammonia solution OBSERVATION Deep yellow color will appear in upper alkaline part INFERENCE Aromatic amine acid is detected Xanthoproteic Test Core Concept

8. EXPERIMENT Take 2ml original solution ( egg white ) + 2 drops conc. H2SO4 + 2ml of acidic mercuric sulphate + mix + boil for 1 min + cool + 1ml NaNO2 + Heat again OBSERVATION Red ppt will appear INFERENCE Tyrosine is detected Millon Nasse’s Test Core Concept

9. Vertical integration (Clinical sciences)

10. Phenylketonuria vv Vertical integration

11. Bioethics

12. Always wear white coat in lab Handle the glassware gently Wait for your turn while working in groups Use gloves while handling chemicals Do not waste reagents or other lab supplies Lab Ethics 13

13. Respect and work in harmony with other students and members of lab Control and prevent contamination in Lab Getting to know your lab mates results in overall healthier relationship, with more intellectual and emotional support. There sho uld be time management and multitasking Maintain Lab etiquettes Communication Skill 14

14. Professionalism Always wear lab coat along with Id card Never take anything from your lab that does not belong to you Follow safety procedures Do not eat or drink during lab working hours

15. The principle of beneficence is the obligation of physician to act for the benefit of the patient and supports a number of moral rules to protect and defend the right of others, prevent harm, remove conditions that will cause harm, help persons with disabilities, and rescue persons in danger. It is worth emphasizing that, the language here is one of positive requirements. The principle calls for not just avoiding harm, but also to benefit patients and to promote their welfare. TAKE HOME MESSAGE Vertical integration Beneficence

16. Suggested Research Article

17. Related Research Article https://r.search.yahoo.com/_ylt=AwrijCh7SShk9CgeLIRXNyoA;_ylu=Y29sbwNiZjEEcG9zAzUEdnRpZANMT0NVSTA5MF8xBHNlYwNzcg--/RV=2/RE=1680390651/RO=10/RU=https%3a%2f%2fwww.nature.com%2fsubjects%2fprotein-analysis%2fsrep/RK=2/RS=9zMx.UbK.1jNkr8pg3VBTN8IaLQ-

18. Skill Lab Assessment 1. Which of the following factors is not responsible for the denaturation of proteins? (a) Heat (b) Charge (c) pH change (d) Organic solvents 2. Which of the following is responsible for specifying the 3D shape of a protein? (a) The peptide bond (b) The amino acid sequence (c) Interaction with other polypeptides (d) Interaction with molecular chaperons

19. 3. ________is not a classified form of conjugated proteins. (a) Lipoproteins (b) Glycoproteins (c) Metalloproteins (d) Complete proteins 4. What is the average molecular weight of an amino acid residue in a protein? (a) 120 (b) 110 (c) 130 (d) 140 Skill Lab Assessment

20. 5. Which of the following statements is true about proteins? (a) Proteins are made up of amino acids. (b) Proteins are essential for the development of skin, teeth and bones. (c) Protein is the only nutrient that can build, repair and maintain body tissues. (d) All of the above Skill Lab Assessment

21. B B D B D Answer Key

22. Steps to Access HEC Digital Library Go to the website of HEC National Digital Library. On Home Page, click on the INSTITUTES. A page will appear showing the universities from Public and Private Sector and other Institutes which have access to HEC National Digital Library HNDL. Select your desired Institute. 5. A page will appear showing the resources of the institution 6. Journals and Researches will appear 7. You can find a Journal by clicking on JOURNALS AND DATABASE and enter a keyword to search for your desired journal. How To Access Digital Library

23. Learning Resources Journal of Practical Vol-1 Google images