Learn Human IL-6R ELISA Kit in 3 minutes

Human IL-6R ELISA Kit developed and produced by EnkiLife.
This kit adopts the classic sandwich ELISA method, which can rapidly, quantitatively and reliably determine the content of Interleukin 6 Receptor in vitro.
EnkiLife provides researchers with a variety of key technical parameters for the kit.
Cat.No: EH10407
Assay: 96-wells plate
Sensitivity: 18.75pg/mL
Target Species: Human
Assay Range: 31.25-2000pg/mL
Sample Type: Serum, plasma and other biological fluids
Synonym: IL6R;CD126;IL-6R-1;IL-6R-Alpha;IL6Q;IL6RA;IL6RQ;gp80

Find out more here: https://www.enkilife.com/product/view/269
Get in touch: [email protected]

1. Human IL-6R ELISA Kit Sandwich ELISA Kit Protocol

2. Human IL-6R ELISA Kit · Pre-coated microplate · A full set of experimental reagents · Reaction Duration: 3.25h · CV: <10% This ELISA kit is rapid, quantitative, reliable ELISA for the determination of Human IL-6R content in vitro.

3. Human IL-6R ELISA Kit Synonym IL6R;CD126;IL-6R-1;IL-6R-Alpha;IL6Q;IL6RA;IL6RQ;gp80 Sensitivity 18.75pg/mL Range 31.25-2000pg/mL Reactivity Human Sample Type Serum, plasma and other biological fluids Assay 96-wells plate

4. Detailed and Easy-to-Follow Protocol 1. Prepare samples and gradient dilution standards.

5. Detailed and Easy-to-Follow Protocol 1. Prepare samples and gradient dilution standards.

6. Detailed and Easy-to-Follow Protocol 1. Prepare samples and gradient dilution standards. 2. Add 100 μ L standard or sample to each well, incubate at 37°C for 90 min. 37 ℃ 90 min

7. Detailed and Easy-to-Follow Protocol 1. Prepare samples and gradient dilution standards. 2. Add 100 μ L standard or sample to each well, incubate at 37°C for 90 min. 37 ℃ 90 min

8. Detailed and Easy-to-Follow Protocol 2. Add 100 μ L standard or sample to each well, incubate at 37°C for 90 min. 37 ℃ 90 min 1. Prepare samples and gradient dilution standards. 3. Discard the liquid, add 100μL Biotinylated Detection Ab solution to each well. Incubate at 37°C for 60 min. 37 ℃ 60 min

9. Detailed and Easy-to-Follow Protocol 2. Add 100 μ L standard or sample to each well, incubate at 37°C for 90 min. 37 ℃ 90 min 1. Prepare samples and gradient dilution standards. 3. Discard the liquid, add 100μL Biotinylated Detection Ab solution to each well. Incubate at 37°C for 60 min. 37 ℃ 60 min

10. Detailed and Easy-to-Follow Protocol 2. Add 100 μ L standard or sample to each well, incubate at 37°C for 90 min. 37 ℃ 90 min 3. Discard the liquid, add 100μL Biotinylated Detection Ab solution to each well. Incubate at 37°C for 60 min. 37 ℃ 60 min 1. Prepare samples and gradient dilution standards. 4. Aspirate and wash the plate for 3 times .

11. Detailed and Easy-to-Follow Protocol 2. Add 100 μ L standard or sample to each well, incubate at 37°C for 90 min. 37 ℃ 90 min 3. Discard the liquid, add 100μL Biotinylated Detection Ab solution to each well. Incubate at 37°C for 60 min. 37 ℃ 60 min 1. Prepare samples and gradient dilution standards. 4. Aspirate and wash the plate for 3 times . 4. Aspirate and wash the plate for 3 times .

12. Detailed and Easy-to-Follow Protocol 2. Add 100 μ L standard or sample to each well, incubate at 37°C for 90 min. 37 ℃ 90 min 3. Discard the liquid, add 100μL Biotinylated Detection Ab solution to each well. Incubate at 37°C for 60 min. 37 ℃ 60 min 4. Aspirate and wash the plate for 3 times . 1. Prepare samples and gradient dilution standards. 5. Add 100μL HRP conjugate working solution. Incubate at 37°C for 30 min. 37 ℃ 30 min

13. Detailed and Easy-to-Follow Protocol 2. Add 100 μ L standard or sample to each well, incubate at 37°C for 90 min. 37 ℃ 90 min 3. Discard the liquid, add 100μL Biotinylated Detection Ab solution to each well. Incubate at 37°C for 60 min. 37 ℃ 60 min 4. Aspirate and wash the plate for 3 times . 1. Prepare samples and gradient dilution standards. 5. Add 100μL HRP conjugate working solution. Incubate at 37°C for 30 min. 37 ℃ 30 min

14. Detailed and Easy-to-Follow Protocol 2. Add 100 μ L standard or sample to each well, incubate at 37°C for 90 min. 37 ℃ 90 min 3. Discard the liquid, add 100μL Biotinylated Detection Ab solution to each well. Incubate at 37°C for 60 min. 37 ℃ 60 min 4. Aspirate and wash the plate for 3 times . 1. Prepare samples and gradient dilution standards. 6. Aspirate and wash the plate for 5 times . 5. Add 100μL HRP conjugate working solution. Incubate at 37°C for 30 min. 37 ℃ 30 min

15. Detailed and Easy-to-Follow Protocol 2. Add 100 μ L standard or sample to each well, incubate at 37°C for 90 min. 37 ℃ 90 min 3. Discard the liquid, add 100μL Biotinylated Detection Ab solution to each well. Incubate at 37°C for 60 min. 37 ℃ 60 min 4. Aspirate and wash the plate for 3 times . 5. Add 100μL HRP conjugate working solution. Incubate at 37°C for 30 min. 37 ℃ 30 min 1. Prepare samples and gradient dilution standards. 6. Aspirate and wash the plate for 5 times .

16. Detailed and Easy-to-Follow Protocol 2. Add 100 μ L standard or sample to each well, incubate at 37°C for 90 min. 37 ℃ 90 min 3. Discard the liquid, add 100μL Biotinylated Detection Ab solution to each well. Incubate at 37°C for 60 min. 37 ℃ 60 min 4. Aspirate and wash the plate for 3 times . 5. Add 100μL HRP conjugate working solution. Incubate at 37°C for 30 min. 37 ℃ 30 min 1. Prepare samples and gradient dilution standards. 7. Add 90 μ L Substrate Reagent . Incubate at 37°C for 15 min. Add 50 μ L Stop Solution. 37 ℃ 15 min 6. Aspirate and wash the plate for 5 times .

17. Detailed and Easy-to-Follow Protocol 2. Add 100 μ L standard or sample to each well, incubate at 37°C for 90 min. 37 ℃ 90 min 3. Discard the liquid, add 100μL Biotinylated Detection Ab solution to each well. Incubate at 37°C for 60 min. 37 ℃ 60 min 4. Aspirate and wash the plate for 3 times . 5. Add 100μL HRP conjugate working solution. Incubate at 37°C for 30 min. 37 ℃ 30 min 6. Aspirate and wash the plate for 5 times . 1. Prepare samples and gradient dilution standards. 7. Add 90 μ L Substrate Reagent . Incubate at 37°C for 15 min. Add 50 μ L Stop Solution. 37 ℃ 15 min

18. Detailed and Easy-to-Follow Protocol 2. Add 100 μ L standard or sample to each well, incubate at 37°C for 90 min. 37 ℃ 90 min 3. Discard the liquid, add 100μL Biotinylated Detection Ab solution to each well. Incubate at 37°C for 60 min. 37 ℃ 60 min 4. Aspirate and wash the plate for 3 times . 5. Add 100μL HRP conjugate working solution. Incubate at 37°C for 30 min. 37 ℃ 30 min 6. Aspirate and wash the plate for 5 times . 1. Prepare samples and gradient dilution standards. 8. Readings at 450nm . Calculation of results. 7. Add 90 μ L Substrate Reagent . Incubate at 37°C for 15 min. Add 50 μ L Stop Solution. 37 ℃ 15 min

19. Detailed and Easy-to-Follow Protocol 2. Add 100 μ L standard or sample to each well, incubate at 37°C for 90 min. 37 ℃ 90 min 3. Discard the liquid, add 100μL Biotinylated Detection Ab solution to each well. Incubate at 37°C for 60 min. 37 ℃ 60 min 4. Aspirate and wash the plate for 3 times . 5. Add 100μL HRP conjugate working solution. Incubate at 37°C for 30 min. 37 ℃ 30 min 7. Add 90 μ L Substrate Reagent . Incubate at 37°C for 15 min. Add 50 μ L Stop Solution. 37 ℃ 15 min 6. Aspirate and wash the plate for 5 times . 1. Prepare samples and gradient dilution standards. 8. Readings at 450nm . Calculation of results.

20. Detailed and Easy-to-Follow Protocol 2. Add 100 μ L standard or sample to each well, incubate at 37°C for 90 min. 37 ℃ 90 min 3. Discard the liquid, add 100μL Biotinylated Detection Ab solution to each well. Incubate at 37°C for 60 min. 37 ℃ 60 min 4. Aspirate and wash the plate for 3 times . 5. Add 100μL HRP conjugate working solution. Incubate at 37°C for 30 min. 37 ℃ 30 min 7. Add 90 μ L Substrate Reagent . Incubate at 37°C for 15 min. Add 50 μ L Stop Solution. 37 ℃ 15 min 6. Aspirate and wash the plate for 5 times . 8. Readings at 450nm . Calculation of results. 1. Prepare samples and gradient dilution standards.

21. Human IL-6R ELISA Kit Sandwich ELISA Kit Protocol