442989644-BC-5000-BC-5150-service-training-material-V1-0-EN-REV-pptx.pptx

[١٣/‏٢ ٣:١٧ م] حسين الشقه: السلام عليكم ورحمه الله عساكم طيبين أن شاء الله

🛑 فيه بعض النقاط حبيت اوضحها لكم في الدور عندنا فياريت الكل يلتزم بها ويأخذ الموضوع بجدية:

- بنسبة لموضوع نظافة الحمامات للدور كامل بشكل عام وبالأخص الحماممات الشقه B ياخي بعد ماتتغسل الماء يجي فوق البراميل طيره ،نجي نحصله مليان، وثاني حاجه القراطيس والاكياس أو موس المكراف حق الحلاقه ليه تطرحه وراء الخلفه حق الحمام كمل منه عطه القمامه أو القراطيس حق الصابون والبكوت حق معجون الاسنان بعد ماتكمل منه عطه القمامه ايش باينقص فيك يام تزقله .

- بنسبة لموضوع الحذي نحنا سوينا كرتون وسوينا خشب على شان تطرح الحذي حقك في المكان المخصص لها نجي نحصل لبس يمين ولبس شمال ولبس فوق ولبس تحت ولبس شرق ولبس غرب فيه مكان مخصص لهن طرحههن فييييييه .

- بنسبة للمغسال والماء الي يطير تحته بعد التغسيل أو تغسيل ماعون الاكل ياااااااخوي بعد ما غلقت من تغسيل السامن حقك اسحب الماء الي يطير تحته ، ماهو كل مره حد بايجي وبايسحب بعدك وباينظف.

- بنسبة لموضوع المغساااااال برضه ممنووووووووووع منعاً باتاً الوضوء في المغساااااال شف المغسال مخصص للغسل اليدات مش للوضوء الصلاة شفه في الحمام عندك بزبوز في الجنب وفيه رشاش وفيه نشاف وفيه براميل طهر فيه براحتك المغسال ممنوووع .

- بنسبة لموضوع الضو حق الحمام أو المغسال هذا لجميع الشقق ياخي بعد ماتستخدم الحمام أو ضو المغسال أو حتى الصاله ياخي قفله قفله قفله شف الكهرباء ماهي بلاش فيه ناس تدفع فلوس أو البطاريه.

- أيضا موضوع دخول الحمام بلحدي الشخصية للحمام ممنوووووووووووووع وبلفم المليان فيه حدي لكل حمام موجودات دخل بهن مش تدخل بحقك وتوسخ الدنيا .

تقريبا هذا ما اسحضرنا

🛑🛑 ياجماعه الخير انا اقول الكلام ذا للكل وانا مش حق امي الدور أو السكن أو الحمام نقول الكلام ذا لكم شفوه والله العظيم ماشي كما الترتيب والنظافة والنظام والسكن حق الكل اذا نحنا ماحافظنا عليه ماحد بايجي من الشارع بانظف وبايرتب لنا كذا.
😔😔ولعفو منكم إذا غلطت على حد أو زليت في كلامي


ياريت الكل يشل الموضوع بجدية 😔😔🌹
[٢٩/‏٢ ٤:٤١ م] حسين الشقه: 🛑
السلام عليكم ورحمة الله وبركاته...
الأخوة الطلاب:
بمناسبة إقتراب إجازة شهر رمضان الكريم وإختلاف الدوامات في كليات وجامعات محافظة عدن وعليه نهيب بالأتي:
1-ضرورة إشعار مندوب الدور بمغادرة الطالب من السكن.
2- الطالب الذي يثبت مغادرته للسكن دون علم أو إشعار يتحمل المسؤولية الكاملة تجاه أي إجراء بحقه بعد رجوعه من الاجازة.
3- على كل مندوب دور ضرورة إشعار مسؤول التغذية بمغادرة الطلاب في دوره حتى يتسنى له تنقيص عددهم من الكشف.
.
.
.
ولكم جزيل الشكر والتقدير.
..
-إدارة_السكن_#
[٢٩/‏٤ ١٢:٠٠ ص] حسين الشقه: السلام عليكم ورحمة الله وبركاته.. .
السطح ممنوع أحد يوقف فيه ولو شفتو أحد قولو له ممنوع يكلم في السطح إطلاقاً وقد تصل عقوبته إننا نطرده إذا ثبت وقوفه جنب الجدار المطل على عمارة الجيران.
🔴ممنوع المكالمات في السطوح
منقول من مدير السكن #[١٠‏/٧‏/٢٠٢٣ ١١:١٨ م] ‏‪[phone]‬‏: السلام عليكم ورحمه الله وبركاته
🚌سوف يتحرك باص فوكسي مكيف ومريح ان شاء الله يوم الثلاثاء
من سيئون ✨الئ ✨عدن*
*💫💫تكييف واصل دون انقطاع راحة تامة💫💫*
طريق شبوة
🏪لتواصل على الارقام التاليه:-
770852545
الله يوفق الجميع🤲🏻
🌹ربي يسعدكم سعادة دائمة😘g
* باص #فخامة _ حضرمي
*نتمنى لكم رحلة سعيدة..
[٢٧‏/١٢‏/٢٠٢٣ ٤:٥٥ م] حسين الشقه: السلام عليكم ورحمه الله وبركاته

الاخوة مt

1. BC-5000/BC5150 service training

2. Content Clinical Overview Principal Installation Software Hardware Liquid system Mechanical structure

3. Blood consists of 40-50% blood cell and 50-60% plasma. Three main parts of blood cell: red blood cell, white blood cell and platelet. clinical—Blood Cells ？

4. Five groups of leukocyte WBC 5-part proportion Lymphocyte 25 ~ 30% Monocyte 3 ～ 8 % Neutrophil 50 ~70% Basophil ～ 1 % Eosinophil 0.5 ～ 3 % clinical—Blood Cells

5. Morphology ： size, shape, cytomembrane, cytoplasma, nucleus, granule. RBC WBC PLT GRAN MONO LYMPH NEU ESO BASO clinical—Blood Cells

6. Clinical—Blood Cells

7. Morphological

8. Clinical—WBC five part comparison NEU EOS BASO LYMPH MONO Diameter 10~14um 11~16um 10~12um 6~9um 12~20um Cytoplasm Large amount more more less More than LYM karyon Multlobed , 2~5 2lobes mostly 3~4lobes, or hard to divide Large round Irregular and indented Granule Neutral Eosinophilic or acidophilic basophilic None normally azurophilic Chromatin More lump More lump More lump Tightness evenly loosen Color of cytoplasm Reddish Reddish Reddish Baby blue Grey blue or little reddish

9. Clinical —granules in cytoplasm Neutral Eosinophilic basophilic Azurophilic Size Fine and even Thick and even Most thick and uneven Thicker than NEU, uneven Shape Fine particles Round or ellipse Differ in shape Differ in shape Amount Much Much Less A few or moderate Color Pale violet red or reddish Orange red or dark yellow Royal purple or dark purple Purple red Distribution Even Even Uneven, coat the nucleus normally Uneven, coat the nucleus occasionally

10. Content Clinics Overview Principle Installation Software Hardware Liquid system Mechanical structure

11. Overview—appearance BC-5150 BC-5000

12. Overview —Dimension 322mm 437mm 386mm BC-2800 324mm 415mm 410 mm BC-5000 vs

13. Overview —Report parameter WBC 11 Para White Blood Cell count WBC Basophils number Bas# Basophils percentage Bas% Neutrophils number Neu # Neutrophils percentage Neu% Eosinophils number Eos# Eosinophils percentage Eos% Lymphocytes number Lym# Lymphocytes percentage Lym% Monocytes number Mon# Monocytes percentage Mon% RBC 8 Para Red Blood Cell count RBC Hemoglobin Concentration HGB Mean Corpuscular Volume MCV Mean Corpuscular Hemoglobin MCH Mean Corpuscular Hemoglobin Concentration MCHC Red Blood Cell Distribution Width Coefficient of Variation RDW-CV Red Blood Cell Distribution Width Standard Deviation RDW-SD Hematocrit HCT PLT 6 Para Platelet count PLT Mean Platelet Volume MPV Platelet Distribution Width PDW Plateletcrit PCT Platelet-large cell ratio P-LCR Platelet-large cell count P-LCC BC5150 only

14. Overview —RUO BC5150 only Name Abbr. Abnormal lymphocytes number ALY# Abnormal lymphocytes Percentage ALY% Large immature cells number LIC# Large immature cells percentage LIC%

15. Overview —appearance 1 、 Display screen 2 、 Power/status indicator 3 、 Probe wipe block 4 、 Sample probe 5 、 [ Aspirate] key Front of the analyzer

16. Overview —appearance 1 、 M-52D diluent inlet 2 、 Waste outlet 3 、 Waste sensor 4 、 AC input socket 5 、 Power switch 6 、 Air outlet for fan 7 、 Back panel Back of the analyzer

17. Overview —Main screen

18. Overview —difference between BC5000andBC5150 item BC-5000 BC-5150 Test speed 40Samples/ h 60Samples/ h Report Para 23 25 RUO NONE 4Para Sample storage 20000 份 40000 份 WBC linear 0.00 × 10 9 /L ～ 100.00 × 10 9 /L 0.00 × 10 9 /L ～ 500.00 × 10 9 /L PLT linear × 10 9 /L ～ 1000 × 10 9 /L 0× 10 9 /L ~ 5000 × 10 9 /L Front panel 043-002705-00 043-002706-00 Screen assembly 115-018417-00 115-018418-00 Key board 043-002703-00 801-3100-00194-00

19. Overview —Specification Measuring mode ： CBC 、 CBC+DIFF Sample mode ： WB Capillary W PD Sampling ： WB Capillary W Sample volume of aspiration ： CBC+DIFF—15ul CBC—11.5ul OV-PD Sample volume of aspiration ： 200ul Diluent dispensed ： 480ul Blood collected ： 20ul Scattergram ： DIFF( Report:L -M) ，（ RUO ： L-W 、 M-W) Histogram ： WBC 、 RBC 、 PLT

20. Operating temperature ： 10 ℃ —30 ℃ Screen ： 10.4’LED ， with touch screen Weight ： ≤ 25Kg Interface ： 4USB, 1 ethemet network port Data transmission ： HL7 supported Printer ： external ， PCL6 printer ： EPSON LQ-590K 、 HP Laser Jet P1505n 、 HP OfficeJet Pro K5300 、 HP LaserJet P1606dn Overview —Specification

21. Reagent Name Type Diluent M-52D DILUENT M-52D Lyse M-52DIFF LYSE M-52DIFF M-52LH LYSE M-52LH Probe cleanser PROBE CLEANSER / Control : Mindray made Calibrator : R&D: S-CAL Mindray made ： S50 Overview —reagent

22. 目录 Clinics Overview Principle Installation Software Hardware Liquid system Mechanical structure

23. Principle —main technology Flow cytometry Cytochemical staining Impedance Lambert–Beer law Laser scattering colorimetry Lyse without cyanide

24. Principle—system block diagram

25. Principle—Flow cytometry Flow cytometry + Laser scattering—WBC 5DIFF Sheath fluid surrounds and forces blood cells suspended in dilution to pass through the center of flow cell one by one. Blood cells pass through the facula of flow cell with high speed

26. Principle—twice accelerating sheath flow 2ed Sheath flow 1 st sheath flow Flow cell Scatter light Laser Blood sample sample

27. Principle— Multi-angle laser scattering Multi-angle laser scattering ； LAS—volume of the cells ； MAS—inner complexity WAS—granularity of the cells

28. Principle— cytochemical staining M-52 DIFF Lyse ： Cationic surfactant To dissolve the RBC ， special treatment to WBC anionic group stain the granular of the WBC More difference in complexity original Lymph Mono Nue Eos Baso After adding reagent

29. M52-LH Lyse To beak down the RBC All the cells are naked (no membrane) except Baso , the Basophils are relatively stable in size and inner structure, larger in volume than other kind of cells, To converts hemoglobin to a hemoglobin complex Principle— cytochemical staining original After adding reagent Lymph Mono Nue Eos Baso

30. Principle— scattergram Lym Eos Mon Neu Baso

31. Principle—L-M Scattergram （ report ） DIFF ： laser scattering+cytochemical staining Lymphocyte Monocyte Neutrophil Eosinophil

32. laser scattering + cytochemical staining scattergram histogram Basophil Principle—histogram of Baso

33. Principle—position of cells Scattergram BAS Histogram

34. Lyse without cyanide Special surfactant ferrous iron is oxidized to ferric iron HGB measurement Lambert-beer law HGB(g/L) = Constant×Log 10 (Blank Photocurrent/Sample Photocurrent) Fe 2+ Fe 2+ Fe 2+ Fe 2+ Fe 3+ Fe 3+ Fe 3+ Fe 3+ Anionic surfactant ＋ Principle—HGB

35. Principle—RBC/PLT Impedance method

36. Content Clinics Overview Principle Installation Software Hardware Liquid system Mechanical structure

37. Installation—procedure Preparation Installation ： check up, unpacking, emplacement, connection, Starting up and login Setting performance verification Background, reproducibility Quality control and calibration(if needed) Scattergram confirmation （ blood sample ） Other setting ： customized setting, maintenance, print. Customer training

38. Installation—preparation Tools ： Blade or clipper USB flash disk Pipette (200ul) Cross-headed screwdriver Control (normal) Calibrator

39. Environment ： should be as free as possible from dust, mechanical vibrations, and electrical interference; Far from brush-type motors, flickering fluorescent lights, and electrical contacts that regularly open and close; Do not place the analyzer on a slope; Do not place the analyzer in direct sunlight or in front of a source of heat or drafts; The environment shall be well ventilated Installation—preparation

40. Installation—preparation Space ： shown as in figure enough room on or below the countertop to accommodate the diluent and waste containers. Voltage ： 100V-240V ～， 50Hz/60Hz Input power ： ≤ 300VA Standard configuration ： Analyzer, reagents Optional ： printer , barcode scanner 50cm 20cm

41. Check ： outer packing and apparatus Packing list and contents Apparatus arrangement Installation-unpacking

42. Waste outlet Power socket Waste sensor connector M-52D diluent inlet Power switch Installation—connection

43. Installation—connection

44. M-52LH lyse connector M-52DIFF lyse connector Installation—connection

45. Peripheral devices ： Printer Barcode scanner Computer Network cable Data management software USB port and network port Installation—connection

46. Hardware self test, liquid system self test and maintenance, background test 。 Installation—Starting up

47. User ID ： service Password ： Se s700 （ capital S, blank between Se and s700) Installation—Starting up

48. Installation—Starting up

49. press press Installation—Setting up reagent

50. Installation—Setting up reagent press Scan or input

51. Installation—Setting up Other settings

52. Background Verification Installation—performance test Parameter Background/ blank count requirements WBC ≤ 0.20  10 9 / L RBC ≤ 0.02  10 12 / L HGB ≤1 g/L HCT ≤0.5 % PLT ≤ 10  10 9 / L

53. Background Verification Installation—performance test

54. Background Verification Installation—performance test

55. Reproducibility Installation—performance test

56. Reproducibility Installation—performance test

57. Installation—quality control press

58. Installation—quality control Click “By SD” to display the limits in the form of absolute value; Or click “By CV” to display the limits in the form of percentage

59. Content Clinics Overview Principle Installation Software Hardware Liquid system Mechanical structure

60. with the engineer permission, factory’s Cal factor and transfer factor will be modified, meanwhile the user’s factor will be adjusted to 100% Factory calibration factor and tansfer factor Software—calibration

61. User’s factor only with the administrator permission, user’s Cal factor is modified, it’s recommend to be with “Admin” permission to login and calibrate Software—calibration

62. HGB gain can be modified manually, to modified the [set value] to change the HGB blank voltage to around 4.5 Target :4.5V Software—gain

63. Software—advanced toolbox Change language Choose the type of data saved Update

64. Software—one key export Analyzer Info, software adjust Info, closed-reagent Info. Save to USB flash disk, files named10000 和 10000_service, if the SN of the analyzer is 10000 U disk ： Kingston 8/16G SanDisk 8/16G Maxell 8/16G FAT32 to reserve 4G space.

65. Software—update Create an upgrade USB drive Unzip update.rar and copy the unzipped “update” directory to the root directory of the USB drive. Upgrade Insert the USB drive into the USB port on the analyzer. Enter the Advanced Toolbox and press[update] to upgrade the software The upgrade process can be divided into two steps. Step 1: upgrade the guidance and operation system Step 2: upgrade the software components When upgrading the boot and operation system, the operator will be prompted to restart the analyzer between step 1 and step 2. When upgrading the software components, the upgrade process will directly go to step 2.

66. Software—communication IP addresses of the PC and the analyzer shall be in the same IP segment Default IP of the analyzer ： 10.0.0.2

67. Software—communication （ Mindray Hematology data management Communication soft ware) Select the actual analyzer model the encoding mode of HL7 communication protocol is UTF-8, and that of 15ID protocol is GBK Analyzer’s IP Port :5100

68. contact your network administrator as to setting up IP address 、 subnet mask and default gateway Software—communication （ LIS ） IP can be changed

69. Content Clinics Overview Principle Installation Software Hardware Liquid system Mechanical structure

70. Hardware—boards Main board(digital control board) Analog drive board Power supply board Indicator board Touch screen control board Laser control board Pre-amplifier board MAS&LAS PD board WAS PD board Liquid sensor board

71. Hardware—system overview

72. Hardware—connection

73. Hardware— Pinster main control board Composition ： Digital section, analog section Function: A/D conversion Converts the analog signals to digital signals which can be processed by FPGA or CPU. Data processing The digital signals obtained from A/D samples are digitally filtered and save by FPGA, then is transmitted to CPU for further processing, output the result. Peripheral interface implementation The CPU module provides a platform for system software, interfaces for peripherals Control interface extension Provides control logic and interface for LCD, SD card and touchscreen , etc

74. Hardware— Pinster main control board

75. Hardware—analog drive board composition ： Power module Control module monitoring module Power drive module

76. Hardware—analog drive board Power module supplies power for all the electronic devices on the analog drive board, including both input power from the analog drive board, and the power converted by the chip; Main control module responsible for communicating with the control board and task scheduling; Monitoring module collecting information such as temperature, pressure, voltage and fluid in the tubes; Power driving module driving electric components such as motor, heater, fan, valves and pumps; RBC detection circuit provides the RBC module with constant current source, RBC signal collection and modulation; Modulation of optical signals and HGB signals.

77. Hardware—analog drive board

78. Hardware—Power supply board Name Socket No. Description D5V J1 PIN1：GND PIN2、PIN3：5V P12V P24V J2 PIN1：GND PIN2：12V PIN3：24V A+12V A-12V J3 PIN1、PIN4：GND PIN2、PIN3、PIN5：-12V PIN6：12V AC120V J4 Alternating current

79. Hardware—Indicator board Indicate the current status of the analyzer by means of sound and light

80. Hardware—status of indicator Analyzer Status Indicator Comments Ready Green light on Sequence is allowed Running Green light flashing Sequence is being performed Running with error Red light flashing An error is present and the system is running Stop with fault Red light on An error occurs and the system is not running No fault is present, but fluidics action is not allowed Yellow light on Initialization and standby status of sequence is not involved in the startup process Enter/Exit standby status Yellow light flashing Enter/Exit standby status

81. Hardware—buzzer Event Buzzer Prompts Comments Startup completed One short beep Startup completed means the whole startup process has been completed and the analyzer is ready for operation Open-vial aspiration completed Two short beeps When count operation can not be started in count related screens (including Sample Analysis, QC, Calibration, Reproducibility, Carryover, Background, Aging and Optical Gain Calibration Count, etc.), press the aspirate key One long beep If these screens have already prompted, then the buzzer need not be responded again. Error Long intermittent beeps Tap the touchscreen to stop the buzzer Analyzer is ready One short beep Analyzer enters ready status from other status When the screen is black prompting “Please power off the analyzer” stop beep If an error occurs during the shutdown process, the buzzer will stop beeping when the screen is black

82. Hardware—touch screen drive board provides the interface between the touchscreen and the control board converting the user's touch operation to identifiable signals for main board. The touch screen drive board needs to be adjusted before use.

83. Hardware— Liquid sensor board LED and Test point TP4 output lower than 0.8V, the LED light is off, (have reagent) output higher than 2V, the LED light is on, (no reagent)

84. Hardware—optical boards Laser control board Pre-amplifier board MAS&LAS PD board WAS PD board

85. Hardware—Laser control board to provide stable and moderate-intensity driving current for laser Power modulation Laser driving current monitoring Laser power control. VR1:to adjust the power of the laser

86. Hardwar—Pre-amplifier board Power modulation I/V conversion Signal modulation.

87. Content Clinics Overview Principle Installation Software Hardware Liquid system Mechanical structure

88. Fluidic system—overview 2 Analysis channel ： WBC&HGB channel, RBC&PLT channel Sample M-52D diluent M-52 LH lyse Impedance Laser scattering RBC/PLT/MCV RBC/PLT WBC/HGB Lymph 、 Mon 、 Eos 、 Neu M-52 DIFF lyse Laser scattering \ colorimetry WBC/BASO/HGB

89. Flowchart （ WB-CBC+DIFF ） Fluidic system—overview

90. Fluidic system—overview Flowchart （ PD -CBC+DIFF ）

91. Fluidic system—reagent consumption Mode Diluent (ml) DIFF lyse (ml) LH lyse (ml) Probe cleanser （ ml ） WB CD 27.5 1 0.2 CBC 23.2 0.2 Capillary_W CD 27.5 1 0.2 CBC 23.2 0.2 Prediluted CD 27.6 1 0.2 CBC 22.9 0.2 Shutdown 60.9 1 0.2 2 Normal startup 65 1 0.2 Wake-up 1 3.7 Wake-up2 16.9 Wake-up3 65 3 0.2

92. Fluidic system—fluidic diagram RBC channel Sampling/dispensing WBC&HGB channel

93. Fluidic system—component Highly integrated air and hydraulic system 17valves ： 1 high pressure (2-way)(V3);1pinch valve(V18) 、 1LVM(V11) 1 syringe assembly (2 syringes ， 250ul 、 10ml ） 1 sampling assembly 2 Electromagnetic Metering pumps （ 1ml 、 200ul ） 2 counting baths 2 isolate chambers 1 flow cell 1 waster pump 1 preheat bath assembly 1 hydraulic pressure sensor 1 check valve 1 filter 1 vacuum chamber

94. VALVE Fluidic system—component

95. Syringe Linkage Name Specification Function Small volume syringe Full scale 250μL Used for quantitative aspiration, distribution and secondary aspiration of blood samples, and for injecting the sample into the flow cell for measurement Large volume syringe Full scale 10mL Used for quantitative addition of diluent to WBC and RBC bath, wipe fluid supply, cleaning of interior and exterior of sample probe and reaction bath, forcing the sheath into the flow cell, cleaning of the flow cell and sample preparation. Syringe Linkage consist of a motor, a driving assembly and two syringes Fluidic system—component

96. Electromagnetic Metering Pump symbol Function ： DP1 ： 1ml ， DIFF lyse adding DP2 ： 200ul ， LH lyse adding symbol Function: Monitoring fluid pressure, give a alert signal when abnormal or beyond settings. Hydraulic pressure sensor Fluidic system—component

97. Preheat bath symbol To heat DIFF lyse ， maintain the Temp in DIFF reaction bath(WBC bath) symbol Tem. Sensor for diluent Monitor Temp of dluent , recommend a target Temp to preheat bath Fluidic system—component

98. Fluidic temperature control Fluidic system—component Item Preheat bath diluent Target Temp Refer to diluent Alarm Temp <target Temp -1.5 >target Temp +3 <10, >40

99. Waste pump drain the wipe block, WBC bath, RBC bath and vacuum chamber, create vacuum in the vacuum chamber Define the flow direction of DIFF lyse in case of reverse flow Attention : direction ！ Check valve Fluidic system—component

100. Sample probe Aspirate and dispense the blood sample and probe cleanser. Note: the sample probe is flat-tipped with a side opening to ensure normal aspiration in case that the tip touches the bottom of the sample tube . Clean the sample probe and drain out the waste fluid while cleaning the inner and outer of the probe Wipe block Fluidic system—component

101. Count reaction bath New structure Easy to maintenance WBC bath ： for sample mixing ,reacting, HGB measuring, provide diluted and reacted sample for DIFF\BASO counting RBC bath ： 50um aperture(improve the accuracy of PLT) Special design to reduce bubble in back bath Consist of aperture, front bath and back bath RBC diluting and mix and counting Fluidic system—component

102. HGB assembly New design Convergent optical path Easy to Maintenance Fluidic system—component

103. Vacuum chamber ： maintain negative pressure for RBC counting. Same with that of BC3000 WBC/RBC Isolation chamber :provide an isolation air room to cut off the internal or external interference Fluidic system—component

104. SV01 Cooperate with DP1 for DIFF lyse suction /drainage SV10 Wipe block drainage ， vacuum pressure release SV02 Cooperate with DP2 for LH lyse suction/ drainage SV11 Control syringe switch to sampling or sample preparation route SV03 On/off between large and mall syringe SV12 RBC bath to vacuum chamber on/off control for RBC counting SV04 On/off the sheath flow SV13 RBC drainage SV05 Control diluent adding to RBC bath SV14 Rinse sample preparation pipe ,RBC bubbling SV06 Bubbling for WBC bath SV15 WBC drainage SV07 RBC bath rinse, cooperate with SV09 to rinse the wipe block SV16 Floe cell drainage SV08 Control syringe suction of diluent , cooperate with SV7/sv9 in dispensing diluent to RBC back bath and wipe block ， PV18 Pinch valve for sample preparation, cooperate with syringe controlling sample preparation and rinse SV09 cooperate with SV7 to rinse the wipe block Valve function Fluidic system—component

105. Fluidic system—functioning and sequence Sampling/dispensing channel Function ： Sampling 、 HGB measurement Rinse the outside of the SP Rinse the inside of the SP Suck and dispense probe cleanser

106. Sampling/dispensing channel Function ： Sampling 、 HGB measurement Rinse the outside of the SP Rinse the inside of the SP Suck and dispense probe cleanser Fluidic system—functioning and sequence

107. Sampling/dispensing channel Function ： Sampling 、 HGB measurement Rinse the outside of the SP Rinse the inside of the SP Suck and dispense probe cleanser Fluidic system—functioning and sequence

108. Fluidic system—functioning and sequence Sampling/dispensing channel Function ： Sampling 、 HGB measurement Rinse the outside of the SP Rinse the inside of the SP Suck and dispense probe cleanser

109. Fluidic system—functioning and sequence WBC&HGB channel Function ： Inject 240ul diluent Dispense 15ul blood + diluent 312ul Suck diluted sample 12ul Add 1ml DIFF lyse Bubble mixing

110. Fluidic system—functioning and sequence WBC&HGB channel Function ： Sample preparation 380ul Adjust the position in the tube Add 200ul LH lyse Bubble mixing

111. Fluidic system—functioning and sequence WBC&HGB channel Function ： To form the sheath flow To boost the sample DIFF count HGB measurement

112. Fluidic system—functioning and sequence WBC&HGB channel Function ： Suck 156.3ul sample from WBC bath Rinse the flow cell Rinse the route of sample preparation anf flush the sample injector and flow cell

113. Fluidic system—functioning and sequence WBC&HGB channel Function ： Sample preparation 800ul To form sheath flow To boost the sample BASO counting Rinse(same as DIFF) WBC bath drainage Inject diluent to WBC bath

114. Fluidic system—functioning and sequence WBC&HGB channel Function ： Rinse (as DIFF) Rinse and drain out WBC bath Inject 2018ul diluent to WBC bath as base solutioon

115. Fluidic system—functioning and sequence RBC&PLT channel Fuction ： Inject diluent (1600ul) Add diluted sample (12ul) to RBC bath Bubble mixing RBC 、 PLT counting

116. Fluidic system—functioning and sequence RBC&PLT channel Function ： Rinse the RBC front bath

117. Fluidic system—functioning and sequence RBC&PLT channel Function ： Rinse the RBC back bath Inject diluent (2560ul) to RBC bath as base solution Release vacuum

118. Fluidic system—sequence in detail Measurement sequence in PD-CBC+DIFF mode Measurement sequence in PD-CBC+DIFF mode is basically the same with the sequence in WB-CBC+DIFF mode, except that: Blood sample has been prediluted outside the analyzer, 200μL of prediluted blood is aspirated in the PD mode Measurement sequence in CBC mode Compared with the sequence in CBC+DIFF mode, the measurement sequence in CBC mode is basically the same except that: Sample volume: 15μL in the WB-CBC+DIFF mode, 11.7μL in the WB-CBC mode.(but for PD mode, CBC+DIFF and CBC mode are the same ) There is no actions related to DIFF measurement, including addition of DIFF reagent, preparation of DIFF samples, DIFF measurement, and cleaning process of the tubes and the flow cell after the DIFF measurement.

119. Fluidic system—sequence for maintenance The orange lines are the locations soaked by the probe cleanser, the blue lines are the locations passed by the probe cleanser Maintenance with probe cleanser(shut down ） enhanced probe cleanser maintenance sequence will be called every 300 times of sample measurement. The soaking time of enhanced maintenance is 6.67S In the Maintenance screen, the probe cleanser maintenance is defaulted as enhanced probe cleanser maintenance.

120. Fluidic system—sequence in detail Starting up rinse The fluidic actions on startup can be divided into three procedures: ： 1. Initialization of fluidics components: initialization of sampling assembly and syringe assembly; 1 metering pump action (without consumption of reagents); creating vacuum and releasing vacuum. 2. Cleanup: including cleaning of all the tubes and components on the analyzer; removing bubbles from the flow cell; discarding 1 ml of DIFF reagent and 0.2 ml of LH reagent. 3. Background: with WB-CBC+DIFF measurement sequence If the background fails, perform the cleanup again and measure the background. For startup after an abnormal shutdown, perform the cleanup twice. 。 Initialization of fluidics components, cleaning up of the interior and exterior surface of the sample probe while starting up after updating

121. Fluidic system—standby The instrument will enter standby status after idling for 15 to 30 minutes (defaulted as 15 minutes). After entering standby status, operations without fluidic actions can be performed from the screen. Exit standby status 1: Standby for less than 1 hour. Clean the exterior of the sample probe and the WBC bath and rebuild the isolation bubble without consumption of lyse . Exit standby status 2: Standby for more than 1 hour and less than 5 hour. Clean the interior and exterior of the sample probe, the WBC bath, the RBC bath, the sample supply tube and the flow cell, and rebuild the isolation bubble without consumption of lyse . Exit standby status 3: Standby for not less than 5 hour. The same as cleanup on startup, including cleaning of all the tubes and components on the analyzer; removing bubbles from the flow cell; discarding 3 ml of DIFF reagent to eliminate the effects of bubbles in the preheat bath tube; discarding 0.2 ml of LH reagent to eliminate the effects of crystal and bubbles.

122. ？

123. 目录 Clinics Overview Principle Installation Software Hardware Optical system Liquid system Mechanical structure

124. Mechanical system—overall layout 2-way valve 3-way valve Syringe assemly 1ml metering pump microswitch [aspirate] key 200ulmetering pump Sampling assembly Fluidic pressure sensor Front view of the main unit (cover opened)

125. Analog drive board Main control board Fan assembly Back view of the main unit (internal structure) Mechanical system—overall layout

126. Optical system Front cover Reagent baffle LH lyse DIFF lyse Net 、 USB interface Power supply assembly Left view of the main unit (left door removed) Mechanical system—overall layout

127. Fluid inlet/outlet Waste pump Vacuum chamber valve LVM valve Diluent Temp. sensor Sampling assembly Front cover RBC bath WBC bath Right view of the main unit (right door removed) Mechanical system—overall layout

128. RBC bath subassembly Bath cap Pan head screw M3X6 Bath holder Bath adjust holder RBC bath Aperture (D50um) Front bath washer RBC back bath Mechanical system—overall layout

129. WBC bath assembly Bath cap Pan head screw M3X6 Bath holder Bath adjust holder WBC bath HGB assembly Mechanical system—overall layout

130. Mechanical system—disassembling RBC bath maintenance assembly

131. pipes are fixed to the holder with cable tie Replace with specific pipe and surround with winding pipe as illustra tion in the figure Winding pipe and adapter should be tied up with cable tie Mechanical system—disassembling

132. pipes are fixed to the holder with cable tie Replace with specific pipe and surround with winding pipe as illustra tion in the figure Winding pipe and adapter should be tied up with cable tie Mechanical system—disassembling

133. Disassembly rear plate of power supply assembly Mechanical system—disassembling

134. Mechanical system—adjustment of bath position

135. Operation Procedure Operation content Quality Control QC Point QC Standard 1.1 Click on “Service → Sample Probe Debug”. Enter the Debug screen as shown in Figure 1.1. 1.2 First, click on the “Initial position” so that the sample probe is above the RBC bath, as shown in Figure 1.2. 1.3 Click on the “WBC bath”(or “RBC bath”) button shown in Figure 1.2. Click the “Middle position” button. Check if the sample probe is basically in the center of the bath. If there is any deviation, loosen the four screws shown in Figure 1.3 and slightly adjust the bath in left/right and front/back directions so that the sample probe is in the center of the bath. Then tighten one screw in each direction Fine adjust the relative position of the bath The sample probe is basically in the center of the bath 1.4 Click on the “Initial position” button, then click “WBC bath”(“RBC bath”) →“Down position” button. Check there is no interference with the bath as the sample probe enters the bath. If the interference occurs, readjust the bath position in accordance with step 1-3 and repeat this step to check. Finally, tighten the 4 screws. Check if the bath position is successfully adjusted The sample probe can not contact with the bath 。 Note: In Figure 1.1, all the buttons except the “Initialization” button will be grey before clicking on Initialization, the three buttons in the “Sample probe horizontal position” column will be illuminated and available for use Only after clicking on Initialization. The “Middle position” and “Down position” buttons will not be available until clicking on the “WBC bath” or “RBC bath” button. Mechanical system—adjustment of bath position

136. Mechanical system—matters need attention All parts of the analyzer are potential infectious, safety protection measures should be taken during operation and maintenance Reagents may irritate eyes, skin and mucous membrane. Operator who contact such items as reagent in the laboratory, should comply with the laboratory safety operating rules, and wear personal protective outfit (such as laboratory protective clothing, gloves, etc.) Once the skin is in contact with Reagent, wash immediately with plenty of water, if necessary, please go to see a doctor; Once the reagent is splashed into your eyes, rinse immediately with plenty of water, and treated by doctors when removing some component with electrostatic sensitive sign, electrostatic ring or static gloves and other protective equipment need to be equipped to avoid damage to the parts Choose the correct specifications and models of tube and screw keep the original tube length. Any p rolongation and shortening are strictly forbidden. Pay attention that the tube and cable could not bend or squeeze, when reinstall, to prevent block or short circuit

137. Thanks ！