Aminoacyl tRNA synthetase

1. Aminoacyl tRNA Synthetase Somanna A. N.

2. Fig: 2 steps of aminoacyl tRNA charging. (a) Adenylylation of amino acid (b) Transfer of adenylylated amino acid to tRNA

3. tRNA charging

4. Classes of Aminoacyl tRNA Synthetases

5. Structure of tRNA : Elements required for aminoacyl synthetase recognition

6. Co-crystal structure of glutaminyl aminoacyl tRNA synthetase with tRNA-Glu Enzyme is shown in grey and tRNA in purple. The yellow, red and green is Glutaminyl AMP

7. Distinguishing features of similar amino acid Accuracy of Aminoacyl tRNA formation

8. Proofreading

9. Editing of Aminoacyl-tRNA . The flexible arm of an aminoacyl-tRNA can move the amino acid between the activation site and the editing site. If the amino acid fits well into the editing site, the amino acid is removed by hydrolysis.

10. Cysteinyl tRNA charged with Cysteine or Alanine