AMPHIBOLIC ROLE OF KREBS CYCLE WITH SPECIAL REFERENCE TO ANAPLEROTIC REACTIONS

Amphibolism in Kreb Cycle

1. AMPHIBOLIC ROLE OF KREBS CYCLE WITH SPECIAL REFERENCE TO ANAPLEROTIC REACTIONS Presented By Morjina Parveen Supervised by- Tridip Boruah Roll No.- 05 Assistant Professor MSc 2 nd semester MC College, Barpeta MC College, Barpeta

2. CONTENTS Amphibolism Amphibolic nature Overview Anaplerosis References

3. WHAT IS AMPHIBOLISM ? Amphibolism can be described as the biochemical pathway where both the processes anabolic and catabolic are involved.

4. Amphibolic nature of Krebs Cycle Contains both catabolic and anabolic reactions. Anabolic- Several intermediates are precursors in biosynthetic pathways. Catabolic- Energy from oxidation of acetyl CoA is stored in reduced coenzymes.

5. An overview of Krebs cycle Discovered by Hans Adolf Krebs . Also called Citric Acid cycle or Tricarboxylic acid cycle. Includes a series of oxidation-reduction reactions in the matrix of mitochondria (eukaryotes). Results in the oxidation of an acetyl group to two molecules of carbon dioxide and reduce the coenzymes that are reoxidized through the electron transport chain.

6. An overview on the anabolic reactions The cycle is engaged in synthetic(anabolic) reactions by following ways : Mitochondrial citrate is transported to the cytosol , where it is cleaved to provide acetyl- CoA for the biosynthesis of fatty acids, sterols. Oxaloacetate and α - ketoglutarate , respectively, serves as precursors for the synthesis of aspartate and glutamate which, in turn, are required for the synthesis of other non-essential amino acids, purines and pyrimidines . Succinyl - CoA is used for the synthesis of porphyrins and heme .

7. An overview on the catabolic reactions Krebs cycle is a catabolic pathway because it oxidizes acetyl- CoA into CO2 and H2O and makes NADH, FADH2 and GTP.

8. Anaplerosis It is a series of enzymatic reations in which metabolic intermediates enter the citric acid cycle from the cytosol . The reaction catalyzed by pyruvate carboxylase that replenishes oxaloacetate to the Krebs cycle is a good example of an anaplerotic or “filling up” reaction.

9. ANAPLEROTIC REACTIONS,

10. REFERENCES Life Sciences by Pranav Kumar and Usha Mina. https://youtu.be/EDWgs?si=QrMlcYnfTdRV6kpM . https://youtu.be/wdXaDrM6Ei0?si=auLyRl40e7RE6_FE.

11. THANK YOU