Anti-idiotype Antibody Production

Antibody idiotope— the unique set of antigenic determinants of the variable region of an antibody. The idiotype represents the variable part of an antibody, including the unique antigen binding site, and the combination of epitopes within the idiotype (i.e., the idiotopes) is unique for each antibody.

1. Anti-idiotype antibodies
(anti-IDs) have specific
binding capabilities to an
idiotope of another
antibody. Anti-idiotypic Antibody: Structure and Types
Approaches to Developing Anti-ID Antibodies
1
Immunized phage
display
library technology: Target antibody is utilized to immunize animals. Isolated immunized
library
Multiple anti-ID antibody discovery approaches
Highest possible affinity
Four types of anti-ID antibodies achie
vable
Large number of anti-ID antibodies for further
selection
Anti-ID antibody sequences are delivered
FEATURES:
ANTI-IDIOTYPE ANTIBODY
Creative Biolabs
Anti-idiotype
Antibody
Production
WHAT WE DO:
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αβ
γε εδ
ζ
CD3
CD4
CD3
ζ
Antigen
Epitope
Paratope
Idiotype
Idiotopes
V
H
V
L
Fab
L-chain
Fc
H-chain
Complementarity deter-
mining regions (CDR), or idiotype can determine the antigen specificity of the antibody. It can be the actual antigen-binding site or comprise variable region sequences outside of the antigenbinding site on the antibody itself.
There are four main classi-
fications of detection
based anti-ID antibodies:
detect free antibody; detect
bound antibody; detect
total antibody; detect T cell
receptor.
Type 1
Type 2 Type 3 Type 4 Welcome
Target antibdoy
Animal immunization
Phage display
Screening & sequencing
Expression & purification
Fab antibodies
Conversion into Ig
Ig antibodies
Ab DNA library
Phage plasmid
Recombinant
phagemid
High affinity
mAbs
Affinity screening
Phage display library
Amplify
E. coli
Screening
Myeloma cells
Hybridoma
Spleen B cell
Harvest mAbs
antibody is
packaged to phage library
and anti-ID antibodies can
then be generated by
biopanning. scFv/Fab
antibodies with an affinity
of 10
-10
M could be
obtained.
Premade non-immunized
human phage display
library technology:
Phage display technology
in the absence of immuni-
zation suits perfectly for
the conformational epitope
targeting anti-idiotypic
antibodies, since
in vitro
selection well maintains the
correctly folded conforma-
tion of target antibody.
Hybridoma technology:
scF
v/Fab forms of target
antibodies are utilized to
immunize rats or mice in
the presence of isotype
matching control antibod-
ies for counter-selection.
Anti-idiotype Antibody Production
Phage Display Library Screening
Native™ Antibody Discovery
Antibody Characterization
Antibody Affinity Maturation
2
Hybridoma Technology
Premade Non-immunized Human Phage Display Library Technology
Immunized Phage Display Library Technology