Anti-Pollution Concept_Presentation_2017-03.pptx

Système d'antipollution dans les véhicules
Pollution du catalyseur et regeneration

1. A nti-Pollution C omprehensive approach by DSM Personal care

2. Facts & Figures Solutions by DSM PC Anti- Pollution claims on the Market 1

3. Facts & Figures 2

4. 3 Pollutants of major public health concern include particulate matter, carbon monoxide, ozone, nitrogen dioxide and sulfur dioxide. By 2050, 70% of the world’s population - 6.4 billion - living in cities Paris Shanghai Los Angeles Pollution is of global concern The world’s biggest environmental health risk 3

5. Awareness & Education : the power of Internet 4 http://aqicn.org/city/beijing/ http://cleanairmakemore.com/tools-downloads/app/ Pollution awareness : The power of internet

6. Anti-Pollution claims on the Market 5

7. Tackles small wrinkles, dull skin tone Minimize dull, unhealthy looking skin Reduce visible effect of pollution Day Damage Protector Smooths and evens out the complexion M ask Urban Aging Corrector S erum Retail markets : What’s launched today! F oundation 6

8. Major effects of pollution on the skin 7 *Extracellular Matrix Perceivable effects Biological effects

9. Solutions by DSM PC 8

10. Our Competence Cleansing 9  Our Concept study focused on biological protection and repair by proven bioactives Three levels of intervention Physical/ biological Pollutants Protecting Repairing 

11. 10 Barrier recovery Skin tone regulation Physical shield Biochemical detox LIPO-GARD Niacinamide PC SERICIN VALVANCE TM SYN ® -UP PREREGEN ® ALPAFLOR ® EDELWEISS Multifactorial causes need multifunctional solutions REGU ® -FADE PEPHA ® -TIGHT PEPHA ® -PROTECT

12. Air pollution impacts skin 11 PM = particulate matter BaP = benzo [a] pyrene O 3 = ozone Biological effects in skin after pollution exposure Oxidative damage Skin barrier disruption Cell viability reduction Inflammation induction Epigenetic modulation F R E E R A D I C A L S BaP PM

13. New data on anti-pollution effects Assay Pollutants Products Effect on skin biology #1 Urban dust PEPHA ® -TIGHT REGU ® -FADE ALPAFLOR ® EDELWEISS PREREGEN ® PF Increased c ell viability #1 Urban dust REGU ® -FADE ALPAFLOR ® EDELWEISS Reduced inflammation markers IL-8, PGE 2 #2 BaP = benzo [a] pyrene * REGU ® -FADE Modulated epigenetic marker microRNA #3 BaP = benzo [a] pyrene * PEPHA ® -PROTECT Prevented DNA damages #4 Ozone PREREGEN ® PF Increased c ell viability 12 *benzo[a]pyrene ( BaP ), a polycyclic aromatic hydrocarbon found in most combustion processes, i.e. in industrial/automotive exhausts and cigarette smoke

14. DSM solutions for Anti-Pollution claims 13 5 actives 5 actions

15. Solutions by DSM PC 14 1- Urban dust particles 2- BaP : benzo [a] pyrene 3- Ozone

16. Solutions by DSM PC 15 1- Urban dust particles 2- BaP : benzo [a] pyrene 3- Ozone

17. 1-DSM Research : Urban dust particles Standard Reference Material ® (SRM) 1649b “urban dust” used in the DSM assay is an authentic research surrogate for urban air pollution of the National Institute of Standards and Technology (Gaithersburg, MD, USA) SRM1649b was prepared from atmospheric particulate material collected in the Washington, DC area. The particles contained certified ingredients, i.e. 23 PAHs (Polycyclic Aromatic Hydrocarbons), 13 PCB (Polychlorinated Biphenyls), 3 heavy metals SRM1649b was resuspended in DMSO (100 mg/ml), stirred for 1 hour . After centrifugation the supernatant was added to cell culture (0.3 mg/ml final). 16 Particle size distribution of SRM1649b PM 10 PM 2.5

18. 1- in vitro urban dust assay by DSM 17 Extracted toxins of urban dust ( SRM1649b) reduced cell viability on human epidermal keratinocytes and induced inflammation. Treatment with DSM actives partially protected against this loss of viability and reduced inflammation markers IL-8 and PGE 2 .

19. 1-Protection against loss of cellular viability Urban dust : SRM1649b extract , Mean ± SD, N= 3, Student t-test, *p  0.05 and ** p 0.01 vs. urban dust stressed cells 18 Urban dust : SRM1649b extract , Mean ± SD, N= 3, Student t-test, *p<0.05 and ** p < 0.01 vs. urban dust stressed cells PEPHA ® -TIGHT REGU ® -FADE ALPAFLOR ® EDELWEISS PREREGEN ® PF

20. 1-Reduced irritation after urban dust stress When human epidermal keratinocytes were stressed by urban dust particles (SRM1649b), induction of inflammation markers was reduced by our actives : Cytokine IL-8 level decreased down to 24% by ALPAFLOR ® EDELWEISS Prostaglandin PGE 2 release declined by 43% by REGU ® - FADE ALPAFLOR ® EDELWEISS: REGU ® -FADE Interleukin 8 (IL-8) Prostaglandin E2 (PGE2) Mean values ± SD, n =3, Student t-test, *p≤0.05 19

21. Solutions by DSM PC 20 1- Urban dust particles 2- BaP : benzo [a] pyrene 3- Ozone

22. 2-Air pollution , epigenetics and skin Epigenetics investigates heritable changes in gene expression occurring without changes in DNA sequence Phenoytypic appearance may result from environmental factors that switch genes on and off and affect how cells express genes . microRNA are small non-coding RNAs that regulate gene expression = molecular gene switches for skin homeostasis Environment Genetics Epigenetics Pollution Stress Toxins… DNA sequence Mutations , SNPs… microRNA DNA methylation Histone modification Skin appearance Lit .: Baccarelli A., Bollati V. Epigenetics and environmental chemicals . Curr Opin Pediatr . 2009 Apr;21(2):243-51. 21

23. 22 2-Epigenetic modulation of skin cells by BaP Human epidermal keratinocytes were stressed with pollutant benzo[a]pyrene ( BaP ) found in combustion processes, i.e. in industrial, car exhausts or cigarette smoke Treated with medium (control) or REGU ® -FADE Analyzed specific microRNAs (epigenetic biomarker) expression profiles for the different treatment conditions

24. 23 2-Epigenetic marker found for senescence Specific miRNA miR-450b-5p could be linked to environmentally stressed changes  miR-450b-5p is associated with cellular senescence in skin aging * p<0.05 vs no BaP / untreated REGU ® -FADE: Bonifacio LN, Jarstfer MB (2010). PLoS ONE 5(9): e12519

25. Human reconstituted epidermis (3D skin model) exposed to BaP (benzo[a]pyrene) to induce oxidative DNA damage PEPHA ® -PROTECT applied topically on skin model Amount of damaged DNA by staining of skin section with 8-oxo-dG 1 antibodies 2-Prevention of environmental DNA damages 24 CONTROL + BaP 0.2% PEPHA ® -PROTECT + BaP The green color intensity shows the amount of DNA damages 2% PEPHA ® -PROTECT + BaP * T-test , significance p<0.05 vs BaP 1 8-oxo-dG: 8-oxo-2'-deoxyguanosine Control *

26. Solutions by DSM PC 25 1- Urban dust particles 2- BaP : benzo [a] pyrene 3- Ozone

27. 3- in vitro Ozone assay by DSM 26 This method aims to evaluate the protective effect of PREREGEN ® PF on human epidermal keratinocytes in the presence of environmental stressor, ozone. Cell culture was treated 18 h with PREREGEN ® PF before a dose of ozone (288 ppm) was applied. After 24 h incubation, cell viability was measured (MTT assay) T= 18 hours T=0 T= 42 hours Active compound added Pollution Stress Induced with ozone Primary Human Epidermal keratinocytes Cell viability measured Tropospheric ozone (O 3 ) is a major highly reactive oxidative pollutant in photochemical smog Formed by human activities via interaction of sunlight, hydrocarbons, VOC and NO x Chronic ozone exposure aggravates skin aging Hüls et al. 2015 Pham et al. 2015 & Lefebvre et al. 2015

28. Cell viability 24 hours after ozone stress (288 ppm): At 2% PREREGEN PF, protection against high dose of ozone was 41% 3-Protective effect in presence of ozone 27

29. New data on anti-pollution effects Assay Pollutants Products Effect on skin biology #1 Urban dust PEPHA ® -TIGHT REGU ® -FADE ALPAFLOR ® EDELWEISS PREREGEN ® PF Increased c ell viability #1 Urban dust REGU ® -FADE ALPAFLOR ® EDELWEISS Reduced inflammation markers IL-8, PGE 2 #2 BaP = benzo [a] pyrene * REGU ® -FADE Modulated epigenetic marker microRNA #3 BaP = benzo [a] pyrene * PEPHA ® -PROTECT Prevented DNA damages #4 Ozone PREREGEN ® PF Increased c ell viability 28 *benzo[a]pyrene ( BaP ), a polycyclic aromatic hydrocarbon found in most combustion processes, i.e. in industrial/automotive exhausts and cigarette smoke

30. DSM solutions for Anti-Pollution claims 29 5 actives 5 actions

31. Origin Leontopodium alpinum ‘Helvetia Nannochloropsis Oculata microalgae, polysaccharide Highly purified Citrullus lanatus Nature-identical pure resveratrol Soja peptides, Superoxide Dismutase Biological Effects Strengthens the epidermal barrier pro- inflammatory stress markers Supports repair & maintenance of ECM DNA protection Multi target mechanism on melanosome Free radical neutralizing, elastase-inhibiting properties Visible Effects Perceivable reduction of skin sensitivity in vivo Provides superior skin firming effect in vivo Reinforcement of the skin defense system in vivo Provides bright and even skin tone in vivo Visibly reduces facial lines and wrinkles Underpinned by scientific discoveries on 5 leading DSM skin care actives A comprehensive solution to fight pollution 30

32. Disclaimer 31 Copyright © 2017 DSM Nutritional Products, 4002 Basel, Switzerland. www.dsm.com/personal-care . All rights reserved. Although DSM has used diligent care to ensure that the information provided herein is accurate and up to date, DSM makes no representation or warranty of the accuracy, reliability, or completeness of the information. This presentation only contains scientific and technical information for business to business use. Country or region-specific information should also be considered when labeling or advertising to final consumers. This publication does not constitute or provide scientific or medical advice, diagnosis, or treatment and is distributed without warranty of any kind, either expressly or implied. In no event shall DSM be liable for any damages arising from the reader’s reliance upon, or use of, these materials. The reader shall be solely responsible for any interpretation or use of the material contained herein. The content of this document is subject to change without further notice. Please contact your local DSM representative for more details. All trademarks listed in this brochure are either registered trademarks or trademarks of DSM in The Netherlands and/or other countries unless explicitly otherwise stated.

33. The 2nd Code: Epigenetic Marks DNA methylations Histone modifications Non-coding RNA (miRNA) Molecular «Gene Switches»: Gene Expression Phenotype

34. Epigenetics - Skin Care of the Future? Already big topic in dermatology Epigenetic factors modifiable or reversible: “ drugable ” Exciting new era in product development Epigenetic Effectors to Skin: Aging Nutrition Rejuventation UV exposure Stress Inflammation Toxins/Pollution Homeostasis Skin Epigenome

35. Cosmetic Products and Epigenetics “The Science of Epigenetic Skin Care” L’Oréal EU Trademark appl. 2007 “Inspired by the field of epigenetics” Olay Pro-X Twins study Most commercial products claiming epigenetics are vaque in describing the relevant science