anticoagulants used in the laboratory for blood test

There is different types of vial with different anticoagulants for different types of blood test

1. (Anticoagulant)

2. (Anticoagulant) Is a substance that prevents blood from coagulation or clotting. The most common anticoagulants used in hematology are: EDTA: Ethylen Diamine Tetraacetic acid Sodium citrate Heparine

3. EDTA and sodium citrate prevent coagulation by removing calcium which is one of the essential factors in coagulation from the blood by precipitation. Heparin acts by forming compiler with plasma anti-thrombin, which inhibits thrombin and other stages of clotting factor activation.

4. EDTA : Its used in a concentration of 1 to 2 mg/ml of blood It may be used for both hematology and chemistry tests, and is the preferred anticoagulant for blood cell counts (CBC), ABO Blood grouping.

5. 2) Sodium citrate: The ratio is one part of 3.8% aqueous solution to nine parts of whole blood. Its used for blood coagulation and platelet function studies (PT &PTT) and can be used for Erythrocyte sedimentation rate (ESR).

6. 3) Heparin : Its concentration is 0.1 to 0.2 mg/ml of blood. Its good for prevention of hemolysis but it is not satisfactory for leukocyte or platelet counts because of cell clumping. Its also produce a trouble some blue background in wrights stain.

7. Color code tube selection of anticoagulants commonly used Stopper color Additive Notes Red No additive Used for blood bank, some chemistries. Collection of serum 10-15 min is required to allow blood to clot before centrifugation Lavender (purple) EDTA Collection of whole blood ( binds calcium)

8. Color code tube selection of anticoagulants commonly used Stopper color Additive Notes Green Sodium or lithium heparin Inhibits thrombin activation. chemistry studies Light blue Sodium citrate Coagulation studies (bind calcium) (PT &PTT) (ESR).