Application of GLC

gas liquid chromatography the most common applications in routine

1. Application of Gas Chromatography

2. Qualitative Analysis: By comparing the retention time/volume of the unknown sample with its standard. By comparing the individual components.

3. Quantitative Analysis: Depends upon the fact the area under a single component peak that is proportional the quantity of the detected component.

4. Separation of Benzene (353.1K) and cyclohexane (353.8K).This separation is virtually impossible by fractional distillation. By GLC, separation has done in a few minutes.

5. Separation of hundreds of hydrocarbons. This separation, which is now routine analysis in petroleum industries, is impossible without GLC .

6. By using molecular sieves, GLC has used to separate the mixture of H2, CO2, CO, O2, CH4, C2H2, C2H4 and C2H6.

7. Automobile exhaust gases that cause main pollutant have analyzed by GLC.

8. Volatile substances such as human breath, environmental air and urine have analyzed by GLC.

9. Flavor and aromas of flowers and food are combination of hundreds of organic compounds in trace amount. These have separated by GLC.

10. GLC has used in separation of radioactive products.

11. High degree of resolution of GLC allows purity of sample to be check. GLC also used to study reaction mechanism.

12. GLC is applicable to species that are appreciably volatile and not stable at room temperature.

13. GLC used for the identification of drug metabolic in blood.

14. The process of identification and separation of biomolecules, gas liquid chromatography has been mostly utilized.

15. In medicine industry, to check the purity and quantity of the medicine GLC has been widely applied.