Application of hyphenated techniques

The hyphenated technique is a combination or coupling of two analytical techniques with the help of a proper interface.
The aim of the coupling is to obtain an information-rich detection for both identification and quantification compared to that with a single analytical technique.

1. Application of Hyphenated Techniques in Drug Analysis Presented By Prof. (Dr.) Dinesh Kr. Mehta MM College of Pharmacy, Maharishi Markandeshwar (Deemed to be University), Mullana , Ambala

2. Hyphenated techniques Hyphenated technique is a combination or coupling of two analytical techniques with the help of proper interface. The hyphenated technique is developed from the coupling of a separation technique and detection technique. The term “hyphenation” was first adapted by Hirschfeld in 1980 to refer to the on-line combination of a separation technique and one or more spectroscopic detection techniques. The aim of the coupling is to obtain an information-rich detection for both identification and quantification compared to that with a single analytical technique . Where it is used

3. Advantages For fast and accurate analysis A Higher degree of automation. Higher sample throughput. Better reproducibility. Reduction of contamination due to its closed system. Separation of quantification at the same time.

4. Types of hyphenated techniques Double hyphenated techniques. Triple hyphenated techniques. Double hyphenated techniques LC-MS LC-NMR LC-IR CE-MS GC-IR GC-MS HPLC-DAD GC-FTIR Combining the two processes reduces the possibility of errors

5. Hyphenated Technique( GC-MS) CONTENTS General Introduction Principle Instrumentation GC-MS Setup Types of Mass Spectrometer detector Application

6. General Introduction Gas Chromatography – Mass Spectrometry (GC-MS) is an analytical method that combines the features of gas chromatography and mass spectrometry to identify different substances with in a test sample. GC-MS separates chemical mixtures into individual components (using a gas chromatograph) and identifies / quantifies the components at a molecular level (using a MS detector). It is one of the most accurate and efficient tools for analyzing volatile organic samples. Mass spectrometer (MS) is an instrument that serves for establishment of the molecular weight and structure of both inorganic and organic compounds, and the identification and determination of analytes in complex mixtures.

7. Principle GC-MS instrument separates chemical mixtures (the GC component) and identifies the components at a molecular level (the MS component). GC works on the principle that a mixture will separate into individual substances when heated/ Partitioning of volatile sample with gaseous mobile phase and liquid stationary phase. The heated gases are carried through a column with an inert gas. As the separated substances emerge from the column opening, they flow into MS. Mass spectrometry identifies compounds by the mass of the analyte molecule.

8. Instrumentation Carrier Gas He ----- It is faster than N and safer than H

9. https://www.google.com/url?sa=i&url=https%3A%2F%2Fwww.researchgate.net%2Ffigure%2FSchematic-plot-of-the-main-components-of-GC-MS-instruments_fig1_273955959&psig=AOvVaw38bp7f0u3qQxHA6c6BAw2L&ust=1628829595992000&source=images&cd=vfe&ved=0CAsQjRxqFwoTCNiO1fXVqvICFQAAAAAdAAAAABAO The GC-MS is composed of two major building blocks: Gas chromatograph Mass spectrometer

10. The molecules are retained by the capillary column and elute from the column at different times. The Mass spectrometer capture, ionize, accelerate, deflect and detect the ionized molecules separately by breaking each molecule into ionize fragments and detecting these fragments using their mass to charge ratio.

11. Food & beverage analysis Types of Mass spectrometer Detectors 1. Quadrupole Mass Spectrometer- most common 2. Ion Trap Mass Spectrometer 3. Magnetic Mass Spectrometer Application Food and beverage Analysis Quantitation of pollutants in drinking and waste water. Environmental analysis GC-MS is a powerful tool for monitoring contaminants in air, water and soil . Oil and gas analysis GC-MS can be used during many stages of petroleum and natural gas testing workflows to determine energy content

12. Application Identification of unknown organic compounds in hazardous waste dumps and reaction products by synthetic organic chemistry Quantitation of drug in metabolites and urine is done for the pharmacological and forensic use. Used for drug analysis, pesticide and herbicide detection Law enforcement (GC-MS is increasingly used for detection of illegal narcotics marijuana, cocaine, opioids, piperazine , etc. and in Sports anti-doping Analysis ) Astro chemistry and Geo chemical Research : Scientist analyzed the atmosphere of venus with the help of GC-MS

13. Medicine and Pharmaceutical Application: GC-MS is widely used in pharmaceutical industries for analytical research and development , quality control, quality assurance, production, pilot plants departments for active pharmaceutical ingredients (API) Clinical toxicology: The toxin and venoms are identified by GC-MS . Academic research: analysis of new compounds for characterization and identification of synthesized or derivatized compound.

14. Academic research Thank You Email: [email protected]