Application Of Recombinant DNA Technology

Application Of Recombinant DNA Technology

1. Asslaam u Alikum

2. Presented By : M Husnain

3. Presentation Topic Application Of Recombinant DNA Technology

4. Application Of Recombinant DNA Technology Recombinant DNA Technology : # A series of procedures used to recombine DNA segments under certain conditions, a recombinant DNA molecule can enter a cell and replicate.

5. Applications Of Recombinant DNA Technology: 1- Gene Cloning : # The recombinant DNA molecule is transferred to a host cell. # Within the host cell it replicates producing dozens of identical copies i.e. it is cloned. # The cloned DNA can be recovered from host cells purified, analyzed and transcribed.

6. 2 – Antibiotics : # Antibiotics are the chemical substance, produced both by microorganisms and synthetically. # They can inhibit the growth of bacteria and others microorganisms and even destroy them. # Peniciline is first antibiotic discovered by Alexander Flemming in 1928 using recombinant technology.

7. 3 – Production of Human Insulin : * Obtaining the human insulin gene: # Human insulin gene can be obtained by making a complementary DNA copy of the messenger RNA for human insulin.

8. 4 – Vaccines : # Vaccine are used to conifer immunity against antigens . # Recombinant DNA technology can be used to clone the gene for the protective antigen protein . # A number of vaccines against virus have been developed using this technology . # These vaccines are Hapatitis B, Foot and Mouth diseases etc. 1- 2-

9. 5 – Gene Therapy : # Genetic engineering may one day enable the medical scientists to replace the defective genes responsible for hereditary diseases e.g. Haemophilia with normal genes.This new system of therapy is called Gene Therapy.

10. 6 – Agriculture : # Genetically engineered plants are developed to resist draught e diseases. # Good quality of food and increased yield of crops is also possible.

11. 7 – Industrial : # In industries recombinant DNA technology will help in the production of chemical compounds of commercial importance, Improvement of existing fermentation processes and production of proteins from waste.

12. 8 – Human Growth Hormones: # Various human growth hormones have been synthesized commercially using Recombinant DNA Technology. # This hormone is used in treatment of Dwarfism.

13. 9 - Animal Husbandry And Sericulture : # For enhancement of milk production in cattle. # For better meat yield in  animals   like pigs, cattle, birds. # For enhancement of silk production in seri culture. # For better egg yield in poultry  birds . # For better wool yield from sheep.

14. 1 0 - Production of Enzymes: # Some useful enzymes can also be produced by recombinant DNA technique. For instance, enzyme urikinase , which is used to dissolve blood clots, has been produced by genetically engineered microorganisms.   11 - Production of Transgenic Animals: # Animals which carry foreign genes are called transgenic animals. Examples: Cow, sheep, goat – therapeutic; human proteins in their milk. Fish like common carp, cat fish, salmon and gold fish contain human growth hormone ( hGH ).

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