Assay demonstrations chloroquine

Assay demonstrations chloroquine Tablet

1. DEMONSTRATION
ASSAY OF ASSAY OF
CHLOROQUINE
PHOSPHATE TABLET
D R . A E J A Z A H M E D M . P H A R M P H . D
D E P A R T M E N T O F P H A R M A C E U T I C A L C H E M I S T R Y
A L I-A L L A N A C O L L E G E O F P H A R M A C Y , A K K A L K U W A

2. AIM
TO PERFORM THE ASSAY OF CHLOROQUINE
PHOSPHATE TABLET METHOD.
(FINISHED PRODUCTS)
•REQUIREMENTS:
•Measuring Cylinder, Burette, Beaker, pipette, volumetric flasks.
•0.1N perchloric Acid Solution, Distilled Water. Chloroquine Tablet

3. THEORY
•ChloroquinePhosphateisanAntimalarial,anti-amoebicdrug.
•Itischemically(RS)-4-(7chloro4-quinolyl-amino)pentyldiethylaminediphosphate.Having
molecularformulaCısH2CIN3,2H3PO,withmolecularweight515.87.
•ChloroquinePhosphateisWhiteoralmostwhite,crystallinepowderwithodourless.
•Itslowlygetsdiscoloredonexposuretolight.
•Itmayexistintwopolymorphicformsdifferingintheirbehaviour,oneofwhichmeltsatabout195°
andtheotheratabout218°,freelysolubleinwater,veryslightlySolubleinchloroform,inethanol
(95%),orinmethanol.
•ChloroquinePhosphatecontainsnotlessthan98.5percentandnotmorethan101.0percentof
C18H26CIN3,2H3PO4calculatedwithreferencetotheanhydroussubstance.

4. •PROCEDURE:
•Weighaccuratelyabout0.2ganddissolvein50mlofanhydrousglacialacetic
acidwiththeaidofheat(ifnecessary,heatunderarefluxcondenser)andcarry
outMethodAfornon-aqueoustitration.
•Determiningtheend-pointpotentiometrically.
•Performablankdeterminationandmakeanynecessarycorrection.
•Factor:Eachmlof0.1Mperchloricacidisequivalentto0.02579gof
C18H26CIN3,2HPO4.

5. PRECAUTIONS
•1.Perchloricacidisusuallyavailableasa70to72%mixturewith
water.Itusuallyundergoesaspontaneousexplosivedecomposition
and,therefore,itisavailablealwaysintheformofasolution.
•2.Beforegoingtoperformassay,thevolumetricglasswareshouldbe
clean.

6. OBSERVATION TABLE
Initial Volume Final Volume
0.00 mL 42 mL
Tablet No. Weight
1 313
2 307
3 309
4 315
5 308
6 312
7 307
8 315
9 312
10 308
11 311
12 307
13 315
14 317
15 308
16 305
17 312
18 309
19 313
20 308
Total Weight = 6208
= 6208 / 20
= 6.208 gm
Equivalent Weight
0.2509--------------0.2
0.1304--------------x
X = 0.2483 gm

7. CALCULATION
•1mlof0.1Mperchloricacidisequivalentto
0.02579gofC15H2CO3,2H3PO4
•%PURITYOFCHLOROQUINE =VOLUMEOF
TITRANTXMOLARITYXEQUIVALENTFACTOR
(WEIGHTOFSAMPLEX100)

8. % PURITY OF CHLOROQUINE
•% PURITY OF CHLOROQUINE = VOLUME OF
TITRANT X MOLARITYX EQUIVALENT FACTOR
(WEIGHT OF SAMPLE X 100)
•% PURITY OF CHLOROQUINE = 42 x 0.1 x 0.24 x 100
•= 100.8 %

9. RESULT
The % Purity Of Chloroquine Phosphate Was
Found To Be -----------%

10. DEMONSTRATION
ASSAY OF
CHLORAMPHENICOL API
D R . A E J A Z A H M E D M . P H A R M P H . D
D E P A R T M E N T O F P H A R M A C E U T I C A L C H E M I S T R Y
A L I-A L L A N A C O L L E G E O F P H A R M A C Y , A K K A L K U W A

11. CHLORAMPHENICOL API
•A= abc
•A= 0.496 -Absorbance 0.125 gm 250 ml = 0.0005 gm/ml = 0.0005*10/250 = 0.002
•a= 297 -Absorptivity
•b =1 -path lenghth
•C=????--Concentration = 0.00167

12. Sr. No Wavelength nm Absorbance
1 278.00 0.496

13. DEMONSTRATION
ASSAY OF
PARACETAMOLE API
D R . A E J A Z A H M E D M . P H A R M P H . D
D E P A R T M E N T O F P H A R M A C E U T I C A L C H E M I S T R Y
A L I-A L L A N A C O L L E G E O F P H A R M A C Y , A K K A L K U W A

14. PARACETAMOLE API
•A= abc
•A = 0. 404 -Absorbance 0.50/100= 0.005 mg/ml 0.00005 mg/ml = 0.0005
•A = 715 -Absorptivity
•b =1 -path lenghth
•C=????--Concentration = 0.00056 % Purity = PY/TY* 100 = 0.00056/0.0005*100 =

17. DRUG ASSAY (ACTIVE INGREDIENTS AND
DOSAGE FORMS)
•Anassayisaninvestigativeprocedureforqualitativelyassessingorquantitatively
measuringthepresence,amount,orfunctionalactivityofatargetentity(Theanalyte).
whichcanbeadrugorbiochemicalsubstanceororganicsample.
•TypesofAssays
1.Chemicalassays
2.Immunoassays
3.Microbiologicalassays
4.Bioassay
•Chemicalassays:Achemicalassayreferstotheanalysisofasamplematerial,calledanalyte,
usingasetofchemicalprocedures•Qualitative-extraction,distillation,precipitationandother
methodsthatdeterminephysicochemicalproperties•Quantitative-volumeorweightofthe
substance

18. CHARACTERISTICS OF A GOOD ASSAY METHOD
•Sensitivity
•Specificity
•Repeatability
•Reproducibility
•Validity
•Stability –tissue has to stay “bioassay FIT

19. CHEMICAL ASSAYS
•Achemicalassayreferstotheanalysisofasample
material,calledanalyte,usingasetofchemical
procedures.
•Qualitative-extraction,distillation,precipitationandother
methodsthatdeterminephysicochemicalproperties.
•Quantitative-volumeorweightofthesubstance

20. TITRIMETRIC ANALYSIS
•Titrimetricanalysisisamethodofanalysisinwhicha
solutionofthesubstancebeingdeterminedistreatedwitha
solutionofasuitablereagentofexactlyknownconcentration.
•Thereagentisaddedtothesubstanceuntiltheamount
addedisequivalenttotheamountofsubstancetobe
determined.Theequipmentusedinthisanalysisare