Barcoding and mobile hardware at CIP Genebank - 2025

Barcoding and mobile hardware at CIP Genebank - 2025. Labels specifications and hardware for barcode kit.

1. CoP-DM Meeting October 2025 Barcoding and Mobile Hardware at CIP Genebank Edwin Rojas (CIP) IT Specialist Lima - Peru

2. A bout CIP Genebank Video (1:49 minutes) https://www.youtube.com/watch?v=cbuK8H7R5yw

3. A bout CIP Genebank CIP Genebank use a QMS (quality management system) to give the users of the Genebank a visible assurance of the quality of the germplasm being distributed, they are cleaned and viruses free. CIP is the custodian of one the world’s largest in vitro collection for Potato, Sweetpotato and Andean roots and tubers crops (ARTC). CIP is the custodian of one of the largest clonal plant cryobanks .

4. About CIP Genebank : Collections Cultivated (Clonal) Potato: 4,900 accs aprox . Sweetpotato : 6,900 accs ARTC: 2,000 accs Wild ( Seed ) Potato : 2,300 accs aprox. Sweetpotato : 1,000 accs ARTC : 500 accs

5. About CIP Genebank: Collection Formats Barcode technology applied in the routinary collection format activities Genebank Seed Collection Collection Formats Plant Collection in Bench Plant Collection in Field

6. About CIP Genebank: Collection Formats Barcode technology applied in the routinary collection format activities Genebank In Vitro Collection Collection Formats Herbarium Collection Cryo Collection 2 cm

7. CIP Genebank website Information about CIP Genebank processes, activities and documentation. https://cipotato.org/ --> Genebank

8. About CIP Genebank Term conservations Term Conservation Format Collection Time Long-term conservation Cryo and seed > 5 years Medium-term conservation In vitro >1 year and < 5 years Short-term conservation Field and greenhouses < 1 year

9. General Specifications for Barcodes and Media Definition and benefits A barcode is a machine-readable representation of information interpreted by the scanner. A barcode help in: A simple way and fast to acquire item information to identify it for example in conservation the accession number A simple way and fast to collect general information of the item for example in germplasm distribution the order number, doi and accession number Track the item for example with the inventory number Therefore, use barcodes in Genebanks is a good data management practice to gain efficiencies 1. CIP 700129 2. 2020-085;CIP 700129;10.18730/8V5~ 3. INV44026

10. Barcode Types Symbologies Description 1D (linear) Code 39 Can encode both numeric and alpha data Code 128 For a wider selection of characters arose than Code 39 2D Data Matrix A Data Matrix Code is a two-dimensional (2D) code that consists of black and white modules. Capacity to recover 20-30% of data Though usually for small size items. Data Matrix Code can store large amounts of data for encoding, making them appropriate for a wide variety of applications. QR A QR Code is another type of 2D code that consists of black modules arranged in a square pattern on a white background. Capacity to recover 20-30% of data Though usually for websites and not for small size items. QR is pretty looks. QR Code can store large amounts of data for encoding, making them appropriate for a wide variety of applications. General Specifications for Barcodes and Media Barcode Types and Symbologies

11. From Genebank Requirements to Best Practices Genebank Requirements: Reduce human errors when handling the germplasm samples improve efficiency of the daily activities Solution: Use an identification system Select a widely used and cheap identification system Use Barcode technology Durable for extended storage Produces long-life image stability Durable enough for transport Facilitate germplasm identification & tracking Apply labels where samples are taken when moving from field/bench to testing labs Barcode label considerations: Best Practices: Print instead writing on labels to eliminate pen-based procedures Scan barcodes instead reading labels to eliminate delays and reduce human errors Barcoding labelling in all procedures to automate operations

12. Barcode label elements and composition Accession number Collecting number Species acronym + Country origin (ISO 3166) Geographical location of sowing Date of samples harvested Field location: Row + Plot number Barcode = Accession number + Field location Example use case: Label of potato tuber harvested and sticked in paper bags. 3.5 cm

13. Barcode label elements and composition Barcode and text (Printed by thermal transfer or direct thermal) Media (Synthetic or paper material) Glue (Removable or permanent)

14. Pointed end labels (stick-ins) 1 roll of 1000 labels to 30 USD Self-tie labels ( loop locks) 1 roll of 1000 labels to 25 USD Self-adhesive labels (square) 1 roll of 4000 labels to 32 USD General Specifications for Barcodes and Media Media types (labels)

15. Self-adhesive Labels Self-tie Labels Pointed End Labels Continue Self Adhesive Labels General Specifications for Barcodes and Media Label layouts and printer sensors For pots For jiffy pots

16. Barcode label specifications Barcode & text Media Glue Seed Collection stored in cold rooms for long-term Collection Formats Plant Collection Stored in Bench Barcode symbology: Data matrix Print technology: Thermal transfer Ribbon type: Resin Period term > 50 years Barcode symbology: Data matrix Print technology: Thermal transfer Ribbon type: Wax + Resin Period term < 3 years Media type: Synthetic polyester Rolls of 4 thousand labels Thickness: <100 microns Label size: 3.3 x 3.5 cm Roll columns: 3 Media type: Synthetic polyplas Rolls of 1 thousand labels Thickness: 200 microns Label size: 191 x 25 mm Roll columns: 4 Glue type: Permanent Glue type: Not use

17. Barcode label specifications Plant Collection Stored in Field Collection Formats Plant Collection in Bench for seed regeneration Barcode symbology: Data matrix Print technology: Direct Thermal Ribbon type: Not use Period term < 6 months Barcode symbology: Data matrix Print technology: Thermal transfer Ribbon type: Wax + Resin Period term < 1 year Media type Synthetic polypropylene Rolls of 350 labels Thickness: 91 microns Label size: 2.0 x 1.0 inches Roll columns: 1 Media type Synthetic polyplas Rolls of 1 thousand labels Thickness: 200 microns Label size: 191 x 25 mm Roll columns: 4 Glue type: Removable Glue type: Not use Barcode & text Media Glue

18. Barcode label specifications Collection Formats In Vitro Collection or for Laboratories Barcode symbology: Data matrix Print technology: Thermal transfer Ribbon type: Wax + Resin Period term < 3 years Media type: Synthetic polypropylene Rolls of 4000 labels Thickness: <100 microns Label size: 3.3 x 3.5 inches Roll columns: 3 Glue type: Removable Barcode & text Media Glue Cryo Collection stored in cold rooms for long-term Barcode symbology: Data matrix Print technology: Thermal transfer Ribbon type: Resin Period term > 50 years Media type: Synthetic Polypropylene Rolls of 4 thousand labels Thickness: <100 microns Label size: 1.500 ” x 0.516 ” Roll columns: 2 in white color Cost: One roll to $130 US dollars (in 2022) Provider: Zebra Label type: Zebra 8000T Cryocool Description: Polypropylene label with a cold-temp permanent acrylic adhesive that offers extremely low-temperature as low as -196°C for liquid nitrogen applications. Glue type: Permanent

19. Rolls of Labels and Ribbons Product: Roll of labels Manufacture: Local provider Labels per roll: 4000 Columns: 3 Printer: Desktop Ribbon type: Resin or Wax/Resin Product: Ribbon Manufacture: Zebra Type: Wax/Resin Printer: Desktop Product: Ribbon Manufacture: Zebra Type: Resin Printer: Desktop Product: Roll of labels Manufacture: Zebra Labels per roll: 350 Columns: 1 Printer: Mobile Ribbon type: Not use Product: Roll of labels Manufacture: DURA-ID Labels per roll: 1000 Columns: 4 Printer: Desktop Ribbon type: Wax/Resin 10 cm 5 cm 10 cm

20. General Specifications for Barcodes and Media Ribbon types Product: Ribbon Wax Model: 5319 PERFORMANCE Manufacture: Zebra Cost: 20 USD Purpose: short-term conservation Product: Ribbon Wax/Resin Model: 3200 PERFORMANCE Manufacture: Zebra Cost: 35 USD Purpose: medium-term conservation Product: Ribbon Resin Model: 5095 PERFORMANCE Manufacture: Zebra Cost: 65 USD Purpose: long-term conservation

21. General Specifications for Hardware Print technologies: Thermal transfer Printing Technology Printer Type Purpose Thermal Transfer Desktop Printer Recommended for genebank activities in special for long-term conservation. Thermal transfer delivers high-definition text, graphic, and bar code print quality for maximum readability and scannability . Thermal transfer printing produces long-life image stability Use ribbon. Ribbon Label

22. General Specifications for Hardware Print technologies: Direct thermal Printing Technology Printer Type Purpose Direct Thermal Desktop Printer Direct thermal printing is an old technology, originally designed for copiers and fax machines, that utilizes chemically coated paper. Not frequently used in desktop printers. Not use ribbon. Mobile Printer Frequently used in mobile printers. Label durability for a maximum of 3 months and used in greenhouses when cross flower plants or in laboratories for petri dishes for seed viability o cryo activities. Not use ribbon. Label

23. Video (1.42 mins): https://www.youtube.com/watch?v=EiQowfHHukg General Specifications for Hardware Resistance norm to define the level of protection of hardware: IP Norm

24. General Specifications for Hardware Mobile computer specifications IPXX = IP+ Code 1 + Code 2 = solid particle protection + liquid ingress protection Product: PocketPC for Field Manufacture/Model: Unitech, HT730 series Level of Protection: IP67 Cost: 1100 USD with accesories scanner

25. General Specifications for Hardware Resistance norm to define the level of protection of hardware: IP Norm IPXX = IP+ Code 1+ Code 2 Code 1 Solid particle protection Code 2 Liquid ingress protection No special protection No waterproofing 1 Protection from solid objects greater than 50 mm in diameter 1 Dripping water 2 Protection from solid objects greater than 12 mm in diameter 2 Dripping water when tilted at 15° 3 Protection from solid objects greater than 2.5 mm in diameter 3 Spraying water 4 Protection from solid objects greater than 1 mm in diameter 4 Splashing of water 5 Complete protection against contact, protection against dust deposit 5 Water jets 6 Complete protection against contact, protection from infiltration of dust 6 Powerful water jets 7 Immersion, up to 1 meter (3 ft 3 in) depth 8 Immersion, 1 meter (3 ft 3 in) or more depth https://en.wikipedia.org/wiki/IP_Code

26. General Specifications for Hardware Mobile printer and mobile computer specifications IPXX = IP+ Code 1+ Code 2 Product: Mobile Barcode Printer Manufacture/Model: Zebra, ZQ600 Series Level of Protection: IP54 Cost: 800 USD Product: PocketPC for Laboratory and Greenhouse Manufacture/Model : Zebra, TC53e series Level of Protection: IP65 Cost: 1600 USD scanner

27. General Specifications for Hardware Barcode scanner and desktop printer specifications IPXX = IP+ Code 1+ Code 2 Product: Handheld Scanner Manufacture/Model: Zebra, DS8100 series Level of Protection: IP52 Cost: 400 USD Product: Desktop Barcode and RFID Printer Manufacture/Model: Zebra, Series ZT411 series Classification: Rugged metal frame and case for demanding activities Cost: 1500 USD

28. Video (45 segs): https://www.youtube.com/watch?v=5eDYjDJwpkE Barcode Use Case 1: Field collection

29. Barcode Use Case 1: Field collection Labels by plot

30. Barcode Use Case 1: Field collection Storage

31. Software: Zebra Designer Pro Cost : $300 aprox. https://www.zebra.com/us/en/products/software/barcode-printers/link-os/zebradesigner-pro.html Barcode Use Case 1: Field collection Components and their specifications Barcode: accession number + ’-’ + plot number Example: CIP 702333-3 Printing type: Thermal transfer

32. Barcode Use Case 1: Field collection Components and their specifications Product: Desktop Barcode and RFID Printer Manufacture/Model: Zebra, Series ZT411 series Classification: Rugged metal frame and case for demanding activities Cost: 1500 USD Printing type: Thermal transfer Self-tie labels (loop locks) Synthetyc of polyplas 1 roll of 1000 labels to 30 USD Sizes: 191 x 255 mm, 4 columns Thickness: 200 microns Product: Ribbon Wax Model: 5319 PERFORMANCE Manufacture: Zebra Cost: 20 USD Purpose: short-term conservation

33. Barcode Use Case 2: Seed collection Video (48 segs) https://www.youtube.com/watch?v=q7ep_tQQs5Q

34. Barcode Use Case 2: Seed collection Storage

35. Barcode Use Case 2: Seed collection Seed count

36. Barcode Use Case 3: In vitro collection Video (2.41 min) https://www.youtube.com/watch?v=aD_ky0KlKOg

37. Barcode Use Case 3: In vitro collection Storage

38. Barcode Use Case 3: In vitro collection Storage

39. Barcode Use Case 3: In vitro collection Subculture evaluation

40. Barcode Use Case 3: In vitro collection Subculture evaluation

41. Barcode Use Case 3: In vitro collection One WIFI antenna for each cold chamber

42. Barcode Use Case 3: In vitro collection Subculture propagation 1 2 3

43. Barcode Use Case 3: In vitro collection Verify labels after subculture

44. Barcode Use Case 3: In vitro collection Barcode encoded Barcode: ’INV’ + inventory id Example: INV 299661 Printing type: Thermal transfer One inventory Separation between inventories to avoid labeling errors Green color for materials free of pathogens.

45. Barcode Use Case 4: Greenhouse Evaluate phytosanitary symptoms Sweetpotato clonal conservation in pots Cross flower of wild crops to regenerate seeds Seed growth in jiffy pots Video (1.11 min) to evaluate phytosanitary symptoms https://www.youtube.com/watch?v=4fL9kjJ6hxo

46. Barcode Use Case 4: Greenhouse Evaluate phytosanitary symptoms

47. Barcode Use Case 4: Greenhouse Cross flower of wild crops to regenerate seeds

48. Barcode Use Case 4: Greenhouse Specifications: Cross flower of wild crops to regenerate seeds Manufacture/model: Zebra PolyPro  4000D Synthetic of polypropylene 1 roll of 250 labels 36 rolls to 250 USD Sizes: 2.0 x 1.0 inches,1 column Permanent glue Product: Mobile Barcode Printer Manufacture/Model: Zebra, ZQ600 Series Level of Protection: IP54 Cost: 800 USD Printing type: Direct thermal Not use ribbon

49. Barcode Use Case 4: Greenhouse Sweetpotato clonal conservation in pots

50. Barcode Use Case 4: Greenhouse Seed growth in jiffy pots

51. Barcode Use Case 4: Greenhouse Specifications: Seed growth in jiffy pots Product: Desktop Barcode and RFID Printer Manufacture/Model: Zebra, Series ZT411 series Classification: Rugged metal frame and case for demanding activities Cost: 1500 USD Printing type: Thermal transfer Product: Ribbon Wax/Resin Model: 3200 PERFORMANCE (35USD) Manufacture: Zebra Cost: 35 USD Purpose: medium-term conservation Pointed end labels (stick-ins) 100 mm x 19 mm, 200 microns 1 roll of 1000 labels to 30 USD Description: Waterproof lightweight non-adhesive stick in (push in) plant tags. Pointed at one end so they can be stuck into plant pots and trays.

52. The International Potato Center (known by its Spanish acronym CIP) is a research-for-development organization with a focus on potato, sweetpotato , and Andean roots and tubers. CIP is dedicated to delivering sustainable science-based solutions to the pressing world issues of hunger, poverty, gender equity, climate change and the preservation of our Earth’s fragile biodiversity and natural resources. www.cipotato.org CIP is a member of CGIAR CGIAR is a global agriculture research partnership for a food secure future. Its science is carried out by the 15 research centers who are members of the CGIAR Consortium in collaboration with hundreds of partner organizations. www.cgiar.org