Basic-Concept-of-Animal-Tissue-Culture-and-Its-History.pptx

tissue culture

1. KULTUR SEL DAN JARINGAN Pertemuan 9 Program Studi S2 Biologi Jurusan Biologi Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Mulawarman Rudy Agung Nugroho

2. Capaian Pembelajaran Mata Kuliah/ Course Outcome : Setelah mengikuti perkuliahan Kultur sel dan jaringan mahasiswa mampu menganalisis falsafah kultur sel dan jaringan melalui diskusi dan literatur secara disiplin dan bertanggung jawab sesuai norma dan etika akademik dalam pengelolaan dan pemanfaatan biodiversitas hutan tropika basah dan lingkungannya. Jumlah pertemuan : 8 Kali Jadwal : Sabtu, 09.30 – 11.10 WITA Ruang : Gedung A lantai 2, Ruang rapat

3. Referensi https://neocloud.co.in/shared/folder/Mjc4ODIw

4. Struktur dasar lipid Anabolisme Katabolisme Jalur Lipid-Glukosa Case study

5. PENILAIAN KULTUR SEL DAN JARINGAN

6. Basic Concept of Animal Tissue Culture and Its History Animal tissue culture is the growth and maintenance of animal cells, tissues, or organs in a controlled, artificial environment. This field has a rich history of scientific advancements and continues to be a crucial tool in various areas of research and medicine.

7. Introduction to Animal Tissue Culture Fundamental Principle Isolating and growing animal cells, tissues, or organs outside their natural environment to study their behavior and properties. Versatility Enables researchers to manipulate and study cells in a controlled setting, leading to insights not possible in whole organisms. Applications Used in fields like cell biology, immunology, cancer research, drug development, and regenerative medicine. Challenges Maintaining the appropriate culture conditions to ensure cell viability and functionality over extended periods.

8. Brief History of Animal Tissue Culture 1 1880s Early experiments by Wilhelm Roux and others to maintain embryonic tissues outside the body. 2 1907 Ross Harrison's pioneering work in culturing frog nerve cells, establishing the foundations of tissue culture. 3 1940s-1950s Advancements in cell culture media and techniques, leading to the establishment of the first immortalized cell lines.

9. Fundamental Principles of Animal Tissue Culture Cell Isolation Extracting and purifying cells from animal tissues using enzymatic or mechanical methods. Culture Media Providing the necessary nutrients, growth factors, and other components to sustain cell growth and function. Controlled Environment Maintaining optimal temperature, humidity, and gas levels to mimic the in vivo conditions.

10. Overview of Cell Lines and Their Characteristics 1 Primary Cells Cells directly isolated from tissues, maintaining their original properties and phenotypes. 2 Immortalized Cell Lines Cells that have been transformed to proliferate indefinitely, often used for long-term studies. 3 Stem Cells Undifferentiated cells with the ability to differentiate into various cell types, valuable for regenerative medicine. 4 Tumor-Derived Cell Lines Cancer cells isolated from tumors, used to understand the biology and treatment of malignancies.

11. Techniques and Equipment Used in Animal Tissue Culture Biosafety Cabinet Provides a sterile work environment to maintain cell culture purity. Incubator Maintains the optimal temperature, humidity, and CO2 levels for cell growth. Microscope Allows for the observation and monitoring of cell behavior and morphology. Pipettes Precise liquid handling tools used for media changes and cell passaging.

12. Applications of Animal Tissue Culture Biomedical Research Understanding disease mechanisms, testing new drugs and therapies, and studying cell biology. Toxicology and Drug Development Evaluating the safety and efficacy of new compounds before in vivo studies. Regenerative Medicine Culturing and expanding cells for tissue engineering and cell-based therapies.

13. Challenges and Limitations of Animal Tissue Culture Contamination Risk of microbial or cross-contamination, requiring strict aseptic techniques. Cell Senescence Primary cells have a limited lifespan, leading to changes in their characteristics. Complexity Maintaining the appropriate culture conditions can be challenging and time-consuming.

14. Future Trends and Advancements in Animal Tissue Culture 1 Organ-on-a-Chip Microfluidic devices that mimic the structure and function of human organs for more accurate disease modeling and drug testing. 2 Stem Cell Therapies Advancements in stem cell culture and differentiation techniques to develop cell-based regenerative treatments. 3 Artificial Intelligence AI-driven analysis of cell behavior and optimization of culture conditions to enhance research efficiency and reproducibility.