Basic Concepts in Laboratory Techniques�PGS 504

Basic Concepts in Laboratory Techniques
PGS 504

1 (0+1)
M. Sc. . (Hort.) Fruit Science + Vegetable Science
3rd Semester
Syllabus and Lecture Plan

Introduction
Laboratory safety rules are a major aspect of every microbiology lab.

Each student in microbiology laboratory must follow specific safety rules and procedures.

Why is Lab Safety Important?

Lab safety rules and symbols are needed so that students do not injure themselves or their classmates.


Lab Safety Rules
Lab Safety Rules
1-Wear protective clothing .

Protective clothes

Gloves are essential.

Lab coats are required.

Safety glasses
( goggles) may be required to avoid splashes.

Lab Safety Rules
Do NOT Wear:

Jewelry

Loose or Baggy clothing



4-Pencils, labels, or any other materials should never be placed in your mouth.
[email protected], [email protected]


5-Caution must be taken when using gas burners. Be sure gas burners are turned off when finished.


6-Long hair must be tied back or covered to minimize fire hazard or contamination of experiments.

Lab Safety Rules
7- Do not eat food or drink water in the lab.
do not use lab glassware as food or water containers.
Lab Safety Rules
8- Protect your hands safety:
- wash hands after every lab.
- Handle glassware, sharp tools and heated containers carefully.


Lab Safety Rules
9- electrical safety:
- unplug electrical equipment after use.
- keep all electrical cords and wires away from water .
Lab Safety Rules
10- Chemical safety:
-never touch, taste or smell a chemical unless instructed to do so.
- never mix chemicals unless instructed to do so.
-keep lids on chemical containers when not in use.
Lab Safety Rules
11-Do not take any cultures out of the lab for any reason.
All cultures should be handled as potentially pathogenic.
Liquid cultures must always be kept in a test tube rack.


Lab Safety Rules
14-Wipe the bench tops down with disinfectant both before you begin your work and after you have completed your work.

Lab Safety Rules
15- Dispose of waste products according to instructions.
16-Report all accidents, no matter how minor, to your supervisor!!


Lab Safety Equipment
Lab Safety Equipment
Safety shower



Eye wash


Lab Safety Equipment

Fire Blanket




Fire Extinguisher





Lab Safety Symbols
Lab Safety Symbols


Biological Safety Levels
Biological Safety Levels

BSL1
BSL2
BSL3
BSL4






Links and References
Quiz
Quiz

Answer 1: c) Define and regulate partnership firms
Answer 2: a) General partnership
Answer 3: c) Partnership Deed
Answer 4: c) Ministry of Corporate Affairs
Answer 5: b) Public limited company
Answer 6: b) AoA
Answer 7: b) Control industrial licensing
Answer 8: c) Competition Act, 2002



THANK YOU

1. Basic Concepts in Laboratory Techniques PGS 504 1 (0+1) M. Sc. . (Hort.) Fruit Science + Vegetable Science 3 rd Semester

2. Topic No. of Lectures Introduction to Laboratory 2 1 1 1 2 2 1 1 1 1 Syllabus and Lecture Plan

3. CO Number CO Statement Taxonomy CO1 Know about terminologies used in the field of law Remember CO2 Understand the importance of Law in business Understand CO3 To apply the knowledge gained in own or any business Apply CO4 + CO5 To analyze and Evaluate the ethical conundrums associated with issues at workplace Analyze and Evaluate

4. Introduction Laboratory safety rules are a major aspect of every microbiology lab. Each student in microbiology laboratory must follow specific safety rules and procedures.

5. Why is Lab Safety Important? Lab safety rules and symbols are needed so that students do not injure themselves or their classmates.

6. Lab Safety Rules

7. Lab Safety Rules 1-Wear protective clothing .

8. Protective clothes Gloves are essential. Lab coats are required. Safety glasses ( goggles ) may be required to avoid splashes.

9. 2-Laboratory personnel should not wear sandals Lab Safety Rules

10. Lab Safety Rules Do NOT Wear: Jewelry Loose or Baggy clothing

11. 3 -Avoid touching objects (e.g., pencils, cell phones, door handles) while wearing gloves. Lab Safety Rules

12. 4-Pencils, labels, or any other materials should never be placed in your mouth.

13. 5-Caution must be taken when using gas burners. Be sure gas burners are turned off when finished.

14. 6-Long hair must be tied back or covered to minimize fire hazard or contamination of experiments. Lab Safety Rules

15. Lab Safety Rules 7- Do not eat food or drink water in the lab. do not use lab glassware as food or water containers .

16. Lab Safety Rules 8- Protect your hands safety: - wash hands after every lab. - Handle glassware, sharp tools and heated containers carefully.

17. Lab Safety Rules 9- electrical safety: - unplug electrical equipment after use. - keep all electrical cords and wires away from water .

18. Lab Safety Rules 10- Chemical safety: -never touch, taste or smell a chemical unless instructed to do so. - never mix chemicals unless instructed to do so. -keep lids on chemical containers when not in use.

19. Lab Safety Rules 11- Do not take any cultures out of the lab for any reason. All cultures should be handled as potentially pathogenic. Liquid cultures must always be kept in a test tube rack.

20. Lab Safety Rules 12- Do not engage in practical jokes or horseplay in the lab.

21. Lab Safety Rules 1 3-Keep nonessential books and clothing far away from your work area.

22. Lab Safety Rules 14-Wipe the bench tops down with disinfectant both before you begin your work and after you have completed your work.

23. Lab Safety Rules 15- Dispose of waste products according to instructions.

24. 16- Report all accidents , no matter how minor, to your supervisor!! Lab Safety Rules

25. Lab Safety Equipment

26. Lab Safety Equipment Safety shower Eye wash

27. Lab Safety Equipment Fire Blanket Fire Extinguisher

28. Lab Safety Symbols

29. Lab Safety Symbols They alert about the possible dangers in the lab.

30. Biological Safety Levels

31. Biological Safety Levels BSL1 BSL2 BSL3 BSL4

32. BSL-1 Not known to cause disease in healthy adult humans Safety equipment Minimal requirements Facilities Open bench top

33. BSL-2 Associated with mild to moderate disease in humans Safety equipment Biological Safety Cabinet and personal protective equipment as needed. Facilities BSL-1 plus the availability of a mechanism for decontamination.

34. BSL-3 Associated with serious or potentially lethal disease in humans Safety equipment Biological Safety Cabinet and personal protective equipment required. Facilities BSL-2 with self-closing double door access and single-pass negative directional airflow.

35. BSL-4 Associated with high risk of life-threatening disease in humans and/or animals Safety equipment Biological Safety Cabinet Full-body air-supplied, positive pressure personnel suit Facilities BSL-3 plus dedicated air and exhaust, decontamination procedures for exit, separate building, etc.

36. Link for study material https:// blog.ipleaders.in /distribution-legislative-powers-union-states/ https:// uk.indeed.com /career-advice/career-development/what-is-business-law https:// blog.ipleaders.in /essentials-of-contract-law-history-and-theory/# Types_of_contracts https://www.concord.app/blog/case-for-contracts-7-reasons/ You Tube Link: https://www.youtube.com/watch?v=vGjrW3WOJYM https://www.youtube.com/watch?v=avCvL3rfW44 Links and References

37. Quiz 1. What is the main objective of the Indian Partnership Act, 1932? - a) Regulate foreign exchange - b) Control monopolistic trade - c) Define and regulate partnership firms - d) Ensure food safety standards 2. Which type of partnership does not require registration under the Indian Partnership Act, 1932? - a) General partnership - b) Limited partnership - c) Joint venture - d) Partnership at will 3. What document is essential for the formation of a partnership? - a) MoA - b) AoA - c) Partnership Deed - d) License 4. Who oversees the implementation of the Companies Act, 1956? - a) RBI - b) SEBI - c) Ministry of Corporate Affairs - d) FSSAI

38. Quiz 5. What type of company requires a minimum of seven members? - a) Private limited company - b) Public limited company - c) One person company - d) LLP 6. Which document outlines the internal regulations of a company under the Companies Act, 1956? - a) MoA - b) AoA - c) Partnership Deed - d) Registration Certificate 7. What is the main purpose of the Industries (Regulation and Development) Act, 1951? - a) Regulate foreign trade - b) Control industrial licensing - c) Define partnership firms - d) Ensure competition in markets 8. Which Act replaced the MRTP Act, 1969? - a) FEMA, 1999 - b) GST Act, 2017 - c) Competition Act, 2002 - d) FSSAI Act, 2006

39. Answer 1: c) Define and regulate partnership firms Answer 2: a) General partnership Answer 3: c) Partnership Deed Answer 4: c) Ministry of Corporate Affairs Answer 5: b) Public limited company Answer 6: b) AoA Answer 7: b) Control industrial licensing Answer 8: c) Competition Act, 2002 

40. THANK YOU