Basic_Concepts_of_Routine_Methods_for_Examining_Tissues.pptx

Basic_Concepts_of_Routine_Methods_for_Examining_Tissues.pptx

1. Basic Concepts of Routine Methods for Examining Tissues An Introduction to Histological Techniques D r . Jagroop Singh PhD Biochemistry Government Medical College Amritsar

2. Introduction Brief overview of histology Importance of tissue examination in medical diagnostics

3. Types of Tissue Samples Biopsies Surgical specimens Cytological samples

4. Fixation Purpose of fixation Common fixatives (e.g., formalin) Steps involved in tissue fixation

5. Processing Dehydration Clearing Infiltration Embedding

6. Sectioning Microtomy and cryotomy Types of microtomes (rotary, cryostat) Preparing thin sections for examination

7. Staining Purpose of staining Common stains (Hematoxylin and Eosin, special stains) Staining techniques

8. Mounting • Preparing slides for microscopy • Mounting media • Cover slipping

9. Microscopy • Types of microscopes (light, electron, fluorescence) • Basic principles of microscopy • How to examine tissue sections under a microscope

10. Special Techniques • Immunohistochemistry • In situ hybridization • Electron microscopy

11. Quality Control • Importance of quality control in histology • Common quality control measures

12. Challenges and Considerations • Common issues in tissue examination • Best practices to ensure accurate results

13. Conclusion • Summary of key points • The role of histology in modern medicine