BASIC LABORATORY Equipment NEEDED IN MICROBIOLOGY.ppt

LABORATORY EQUIPMENTS

1. Lab EquipmentLab Equipment

2. BeakerBeaker
Beakers hold solids or Beakers hold solids or
liquids that will not liquids that will not
release gases when release gases when
reacted or are unlikely reacted or are unlikely
to splatter if stirred or to splatter if stirred or
heated.heated.

3. Beaker TongsBeaker Tongs
Beaker tongs are Beaker tongs are
used to move beakers used to move beakers
containing hot liquidscontaining hot liquids

4. Erlenmeyer FlaskErlenmeyer Flask
Erlenmeyer flasks hold Erlenmeyer flasks hold
solids or liquids that may solids or liquids that may
release gases during a release gases during a
reaction or that are reaction or that are
likely to splatter if likely to splatter if
stirred or heated.stirred or heated.

5. Florence FlaskFlorence Flask
Rarely used in first year Rarely used in first year
chemistry, it is used for chemistry, it is used for
the mixing of chemicals. the mixing of chemicals.
Narrow neck prevents Narrow neck prevents
splash exposure.splash exposure.

6. Graduated CylinderGraduated Cylinder
A graduated A graduated
cylinder is used to cylinder is used to
measure volumes of measure volumes of
liquids.liquids.

7. Test TubesTest Tubes
13 x 100 mm test tubes13 x 100 mm test tubes
10 x 75 mm test tubes10 x 75 mm test tubes
IgnitionIgnition
tubetube

8. Test Tube HolderTest Tube Holder
A test tube holder is A test tube holder is
useful for holding a useful for holding a
test tube which is test tube which is
too hot to handle.too hot to handle.

9. Test Tube BrushesTest Tube Brushes
Test tube brushes are Test tube brushes are
used to clean test tubes used to clean test tubes
and graduated cylinders.and graduated cylinders.
Forcing a large brush into Forcing a large brush into
a small test tube will a small test tube will
often break the tube.often break the tube.

10. Test Tube RacksTest Tube Racks
Test tube racks are for holding Test tube racks are for holding
and organizing test tubes on and organizing test tubes on
the laboratory counter. the laboratory counter.
After washing flip test tube After washing flip test tube
over on wooden peg to dry.over on wooden peg to dry.

11. Rubber StoppersRubber Stoppers
Rubber stoppers are used Rubber stoppers are used
to close containers to avoid to close containers to avoid
spillage or contamination.spillage or contamination.
Containers should never be Containers should never be
heated when there is a heated when there is a
stopper in place.stopper in place.

12. Spot PlatesSpot Plates
Spot plates are used when Spot plates are used when
we want to perform many we want to perform many
small scale reactions at small scale reactions at
one time. one time.

13. Glass Stir RodGlass Stir Rod
A glass rod is used to A glass rod is used to
manually stir solutions. manually stir solutions.
It can also be used to It can also be used to
transfer a single drop of transfer a single drop of
a solution.a solution.

14. ForcepsForceps
Forceps (or tweezers) are used to pick up small Forceps (or tweezers) are used to pick up small
objects.objects.

15. FunnelFunnel
A funnel is used A funnel is used
to aid in the to aid in the
transfer of liquid transfer of liquid
from one vessel from one vessel
to another.to another.

16. Watch GlassWatch Glass
A watch glass is used to A watch glass is used to
hold a small amount of hold a small amount of
solid, such as the solid, such as the
product of a reaction.product of a reaction.

17. Wash BottleWash Bottle
A wash bottle has a A wash bottle has a
spout that delivers a spout that delivers a
wash solution to a wash solution to a
specific area. Distilled specific area. Distilled
water is the only liquid water is the only liquid
that should be used in a that should be used in a
wash bottle.wash bottle.

18. Weighing BoatWeighing Boat
Weighing boats are used to Weighing boats are used to
weigh solids that will be weigh solids that will be
transferred to another transferred to another
vessel.vessel.

19. Bunsen BurnerBunsen Burner
Bunsen burners are used Bunsen burners are used
for the heating of for the heating of
nonvolatile liquids and nonvolatile liquids and
solids.solids.

20. StrikersStrikers
Strikers are used to Strikers are used to
light Bunsen burners. light Bunsen burners.
The flints on strikers are The flints on strikers are
expensive. Do not operate expensive. Do not operate
the striker repeatedly the striker repeatedly
just to see the sparks!just to see the sparks!

21. Evaporating DishEvaporating Dish
The evaporating dish is The evaporating dish is
used for the heating of used for the heating of
stable solid compounds stable solid compounds
and elements.and elements.

22. CrucibleCrucible
Crucibles are used for Crucibles are used for
heating certain solids, heating certain solids,
particularly metals, to particularly metals, to
very high temperatures.very high temperatures.

23. Clay TriangleClay Triangle
The clay triangle is used The clay triangle is used
as a support for as a support for
porcelein crucibles when porcelein crucibles when
being heated over a being heated over a
Bunsen burner.Bunsen burner.

24. Crucible TongsCrucible Tongs
For handling hot crucibles; For handling hot crucibles;
also used to pick up other also used to pick up other
hot objects. hot objects. NOTNOT to be to be
used for picking up used for picking up
beakers! beakers!

25. Ringstands and their ComponentsRingstands and their Components
Ringstands are a safe and Ringstands are a safe and
convenient way to perform convenient way to perform
reactions that require heating reactions that require heating
using a Bunsen burner.using a Bunsen burner.
RingstandRingstand

26. Ringstands and their ComponentsRingstands and their Components
Utility ClampsUtility Clamps
Utility clamps are used to Utility clamps are used to
secure test tubes, secure test tubes,
distillation columns, and distillation columns, and
burets to the ringstand.burets to the ringstand.

27. Ringstands and their ComponentsRingstands and their Components
Iron RingIron Ring
Iron rings connect to a Iron rings connect to a
ringstand and provide a ringstand and provide a
stable, elevated platform stable, elevated platform
for the reaction.for the reaction.

28. Ringstands and their ComponentsRingstands and their Components
Wire GauzeWire Gauze
Wire gauze sits on the iron Wire gauze sits on the iron
ring to provide a place to ring to provide a place to
stand a beaker. stand a beaker.

29. Ringstands and their ComponentsRingstands and their Components
Double Buret ClampsDouble Buret Clamps
Double Buret Double Buret
clamps are used to clamps are used to
burets – long burets – long
graduated tubes graduated tubes
used in titration.used in titration.

30. Triangular FileTriangular File
Triangular files are Triangular files are
used primarily to cut used primarily to cut
glass rod, a skill glass rod, a skill
that your instructor that your instructor
will share with you will share with you
when it becomes when it becomes
useful.useful.

31. Litmus PaperLitmus Paper
RedRed litmus paper is litmus paper is
used to identify used to identify
bases.bases.
BlueBlue litmus paper is litmus paper is
used to identify used to identify
acids.acids.

32. Gas Collecting BottleGas Collecting Bottle
We use gas collecting bottles We use gas collecting bottles
when large volumes of gases are when large volumes of gases are
produced, and must be collected produced, and must be collected
by the displacement of water.by the displacement of water.

33. Medicine DropperMedicine Dropper
A medicine dropper is A medicine dropper is
used to transfer a used to transfer a
small volume of liquid small volume of liquid
(less than one mL).(less than one mL).
On top of each medicine dropper is a “On top of each medicine dropper is a “rubber bulbrubber bulb””

34. Mohr PipetMohr Pipet
A Mohr pipet A Mohr pipet
measures and measures and
delivers exact delivers exact
volumes of liquids.volumes of liquids.

35. Glass PlatesGlass Plates
Glass plates provide a Glass plates provide a
surface for semi-micro surface for semi-micro
scale experiments, scale experiments,
such as drop reactions such as drop reactions
and testing of acids and testing of acids
and bases.and bases.

36. SpatulasSpatulas
Spatulas are used to Spatulas are used to
dispense solid chemicals dispense solid chemicals
from their containers. from their containers.
Chemicals should never Chemicals should never
be transferred with be transferred with
your bare hands.your bare hands.