Basics of uv spectroscopy

Basics of ultra-voilet spectroscopy

2. Presentation
Basics Of Ultravoilet Spectroscopy

3. Presented By:
Rahat Inayat Ali 14CH23

4. Contents
* Spectroscopy
* Ultravoilet Spectroscopy
* Ultravoilet Radiation
* Principle of UV spectroscopy

5. Spectroscopy
•Spectroscopy is the
study of the
interaction between
matter and
electromagnetic
radiation. Historically,
spectroscopy
originated through the
study of visible light
dispersed according
to its wavelength, by
a prism.

6. Ultravoilet Spectroscopy
•Ultravoilet and Visible (UV-Vis) absorption
spectroscopy is the measurement of the
attenuation of a beam of light after it
passes through a simple or after reflection
from a simple surface.Absorption
measurement can be at a single
wavelength over an extended spectral
range.

7. Why we use UV Spectroscopy?
•Detection of functional groups
•Detection of Impurities
•Qualitative Analysis
•Quantitative Analysis
•Single Compound Without Chromophore
•Drugs with chromophoric reagent
•It is helps to show the relationship
between different group, it is useful to
detect the conjugation of the compounds.

8. Ultravoilet Radiation
•Ultraviolet (UV) light is an
electromagnetic radiation with a
wavelength from 400 nm to 10 nm,
shorter than that of visible light but
longer than X-rays.
•Where does it come from?
•The sun is a major source of
ultraviolet rays. Though the sun
emits all of the different kinds of
electromagnetic radiation, 99% of
its rays are in the form of visible
light, ultraviolet rays, and infrared
rays (also known as heat). Man-
made lamps can also emit UV
radiation, and are often used for
experimental purposes.

10. Principle of UV spectroscopy
•Ultravoilet absorption spectra arise from
transition of electron with in a molecule
from a lower level to higher level.
•A molecule absorb UV radiation of
frequency, the electron in that molecule
undergo transition from a lower level to
higher level.
•The energy can be calculated by equation.
»E=hv erg

11. Thank You