BDS Spotters Mycology pptx practical examination

Bds Spotters

1. SPOTTERS-MYCOLOGY

2. 1. Candida albicans Yeast like Fungus Microscopy: Ovoid or spherical in shape Gram positive organism , produce budding yeast cells with pseudohyphae Culture on SDA: Colonies are Creamy white, smooth and dry with yeast odour.

3. 2. Aspergillus flavus On SDA: Velvetty yellow to brown colonies On LPCB mount: Septate hyphae , phialides cover the entire vesicle.

4. 3. Aspergillus fumigatus On SDA: Smoky green colonies On LPCB mount: Septate hyphae , Smoky conidiophores. Phialides are uniseriate and cover the upper half of the vesicle

5. 4. Aspergillus niger On SDA: Wooly, white to yellow and become brown to black. On LPCB mount: Septate hyphae , Smoky conidiophores. Phialides are biseriate, covers the entire vesicle. Conidia are black.

6. 5. Penicillium spp. On SDA: Velvetty white, later become powdery blue-green colonies. On LPCB mount: Septate hyphae, Metullae gives rise to conidium Brush bottle appearance

7. 6. Mucor On SDA: Cottony (fluffy appearance) and pure white colonies. On LPCB mount: Aseptate hyphae(broad), Rhizoids are absent Sporangium contains sporangiophores.

8. 7. Rhizopus On SDA: Cottony grey white colony, later becomes brown black. On LPCB mount: Aseptate hyphae. Rhizoids are seen below the sporangiophores. Sporangiophore with distinct columella