Bhi Media supplemented w 005-sps

Bhi Media is used for detection of microorganisms in blood. BHI Supplemented w/ 0.05% is used for detection of microorganisms, including aerobic and anaerobic bacteria. Brain Heart Infusion Broth is a modification of the original formulation of Rosenow,

1. TM Media
904, 9th Floor, Bigjos Tower,
Netaji Subhash Place,
Delhi, 110034
BHI SUPPLEMENTED
W/ 0.05% SPS
INTENDED USE
BHI Media SUPPLEMENTEDW/ 0.05% SPS For detectionof microorganisms in blood
COMPOSITION
IngredientsGms/Ltr.
Peptone 10.00
Beef heart, infusion from9.80
Brain, infusion from 7.70
Sodium chloride 5.00
Disodium phosphate 2.50
Dextrose 2.00
Sodium polyanethol sulphonate0.50
Instructions for use
Collectthesamplefrompatientsbysyringe.Injectthesampleinthevialaseptically.Mix
properly. Incubate and keep undisturbed.
Appearance:Light to medium amber colour, clear solutionwithout any precipitation

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pH (at 25°C):7.4 ± 0.2
PRINCIPLE
BHIMediaSupplementedw/0.05%isusedfordetectionofmicroorganisms,including
aerobicandanaerobicbacteria.BrainHeartInfusionBrothisamodification ofthe
originalformulationofRosenow,whereheaddedpiecesofbraintissuestodextrose
broth.BrainHeartInfusionBrothisalsothepreferredmediumforanaerobicbacteria,
yeastsandmoulds.BHIMediaisespeciallysuitedforthecultivationofstaphylococci
fortheplasmacoagulasetestandforsettingupbloodcultures.Additionofascites
permitsthecultivationofgonococci.Withtheadditionof10%defibrinatedsheepblood,
itisusefulforisolationandcultivationofHistoplasmacapsulatumandotherfungi.For
selectiveisolationoffungi,additionofgentamicinand/orchloramphenicolis
recommended.BHIbrothisalsousedforpreparingtheinoculumusedinantimicrobial
susceptibility tests.
Beefheart,BraininfusionsandPeptoneprovidesorganicnitrogen,carbon,andvitamins.
Dextroseisacarbohydratesource.Lowconcentrationofdextroseisusedtostimulate
earlygrowth.Sodiumchloridemaintainstheosmoticenvironment.Disodiumphosphate
is a buffering agent.
BHI Broth can be supplemented with Sodium polyanetholsulfonate(SPS) which is used as a
non-toxic anticoagulant which enables bacterial growthand prevents the action of natural
bacterial inhibitors of blood.
Microbiological parameters (Growth promotion test)
Cultural characteristics observed after inoculation(103 CFU/ml) and incubation at 35 -
37°C after 24 - 48 hours.

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INTERPRETATION:
Culture characteristics observed after incubationperiod of 18 - 24 hours at 35 ± 2°C
Test strains ATCC Inoculum (CFU) Growth
Enterococcus faecalis 29212 10^3 Luxuriant
Streptococcus pyogenes 19615 10^3 Luxuriant
Streptococcus pneumoniae6303 10^3 Luxuriant
Neisseria meningitidis 13090 10^3 Luxuriant
Reference
1. Rosenow, Dental Research, 1, 205 (1919)
2. Rosenburg T. et al, J. Inf. Dis., 74, 131 (1944)
3. Mc Faddin J.F., Media for Isolation-Cultivation-Identification-Maintainance of medical
Bacteria, Vol. I, Williams and Wilkins, Baltimore(1985)
4. Lennette, Balows, Housler and Shadomy (Eds.), Manualof Clinical Microbiology, 4th
ed., ASM, Washington, D.C. (1985)
5. 5. Ajello L., George L., Kaplan W. and KaufmanL., CDC Laboratory Manual of Medical
Mycology, Atlanta, Ga.: US. DHEW, Center for DiseaseControl (1966)
6. McDonough E., Geoge L., Ajello L. and BrinkmanS., Mycopathol. Mycol. Appl.; 13, 113
(1960)
7. Production of 12α-hydroxysteroid dehydrogenasefrom Clostridium group P: I.A.
MacDonald, Experientia 37, 451 (1981)

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