Biochemistry and biotechnology of research in TRENDS

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1. 23011760-018 HAJRA BIBI COVALENT INHIBITION OF STREPTOCOCCAL GLUCAN SUCRASES FOR DISRUPTING ORAL BIOFILM DEVELOPMENT

2. BACKGROUND Covalent Inhibition Streptococcal Glucan Sucrases Oral Biofilm Development Covalent Inhibition for Disruption

3. GLUCAN SUCRASE Glucan sucrases , from Streptococcus mutans (such as GtfB , GtfC , and GtfD ) Domains Catalytic Domain: Glucan -Binding Domain : N-terminal Signal Peptide : Sites for Covalent Inhibition Aspartic Acid (Asp) Glutamic Acid ( Glu )

4. INTRODUCTION   Dental Caries And Biofilm Formation: Without glucans , the biofilm matrix becomes less stable, leading to reduced adhesion of streptococcal cells and potentially increased susceptibility to removal by mechanical forces or other antimicrobial agents . Role of Glucan Sucrases : Streptococcal glucan sucrases are enzymes responsible for synthesizing glucans from sucrose. These glucans are crucial for the formation and maintenance of the biofilm matrix in oral environments . Covalent Inhibition Mechanism: Covalent inhibitors form a stable, irreversible bond with the active site of enzymes, effectively rendering the enzyme inactive. By targeting glucan sucrases , these inhibitors can prevent the production of glucans , thereby disrupting biofilm formation . Potential Impact: This approach aims to create new targeted treatments that specifically address the mechanisms behind microbial pathogenicity without disturbing the overall balance of the oral microbiome.

5. HYPOTHESIS Covalent inhibition of streptococcal glucan sucrases will significantly disrupt the development of oral biofilms by interfering with the synthesis of extracellular polysaccharides, which are essential for biofilm structural integrity and stability

6. RESEARCH OBJECTIVE

7. METHODS

8. EXPECTED OUTCOMES

9. CONT….

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