BIOCHEMISTRY LAB INSTRUMENTS - Copy.pptx

For spotting in mbbs 1st year viva exams

1. BIOCHEMISTRY LAB INSTRUMENTS BY-ITIKA SAXENA

2. VOLUMETRIC GLASSWARES The Volumetric glasswares are Pipettes, Burettes, Measuring Cylinder, Bottle top dispenser and Standard Flask’s.

3. 1. MOHR PIPPET OR FIXED VOLUME PIPPET

4. Commonly used for the preparation of reagents. This are used to measure greater volumes like 50 mL , 100 mL , 500 mL , 1L, 2 L etc. 2. Measuring Cylinder:

5. Non-volumetric glassware’s are Test tubes, Beaker, Conical Flask, Round Bottom Flask and Petri dish. Non-Volumetric Glasswares

6. 1. Test tubes:

7. 2. CONICAL FLASK

8. URINOMETER Instrument is used to measure specific gravity. It is calibrated for 20 ̊C temperature. For every 3 ̊C rise in temperature, 0.001 is added to reading. For every 3 ̊C fall in temperature, 0.001 is substracted to reading.

9. No preservative/anticoagulant. Uses: usually for serum chemistry testing, serology and blood bank testing Red (Plain tube) VIAL

10. EDTA (Ethylene Diamine Tetra-Acetate) liquid: EDTA  acts as an anticoagulant, binding the calcium ions. These tubes are used primarily in haematology procedures, assessing complete blood count, bone marrow Specimens, HbA1C .. LAVENDER TOP TUBE

11. SST (Serum Separator Tube) Contains a gel at the bottom to separate blood cells from serum on centrifugation. Uses: LFT, CRP, Urea, Electrolyte, Immunology, and Serology . SST/ Gold top tube:

12. Contains two main agents, Sodium fluoride acts as an antiglycolytic agent to ensure that no further glucose breakdown occurs within the sample after it is taken. Potassium oxalate acts an anticoagulant. Common Tests— Glucose—this can be fasting or non fasting, or part of glucose tolerance test (GTT) Lactate GREY VIAL

13. Contains Anticoagulant Sodium citrate Uses: Coagulation(clotting) studies and platelet function. Light Blue :

14. Sodium Heparin or Lithium Heparin anticoagulant or ammonium heparin . Acts as an Anticoagulant by inhibiting thrombin formation. Uses: for collection of heparinized plasma or whole blood for special tests. Dark GREEN

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