Biochemistry Laboratory.docxllllllllllllllllllllllllllllllllll

biochem for evryone

1. Biochemistry Laboratory – Make-Up Activity
Name: _______________________________________________ Date Submitted:
________________
Instructions:
As a make-up requirement for missing the midterm quiz, submit a written reflection/report on
one (1) of the following laboratory topics:
Lab 1: Membrane Transport System
Lab 2: Water Properties and Buffer
Lab 3: Colloids and Their Properties
Lab 4: Carbohydrates
Choose only one topic and write a 1–2 page reflection report including the parts below:
Required Parts:
1.Title of the Experiment
2.Purpose / Objective – What was the goal or concept of the experiment?
3.Summary of the Experiment – Briefly describe what was done and observed.
4.Expected Results – What results were expected or obtained?
5.Reflection and Interpretation – What did you learn or realize from this experiment? How
does it relate to biochemistry concepts and real-life applications (e.g., health sciences)?
Format:
Length: 1–2 pages
Font: Arial or Times New Roman, size 12
Spacing: 1.5 lines
File format: PDF (or handwritten and scanned)

2. Grading Rubric (Total: 20 points)
Criteria Description Points
Completeness Includes all required parts4
Content Accuracy &
Understanding
Shows clear grasp of the
chosen lab topic
6
Reflection & InterpretationThoughtful insights and
connection to concepts
6
Organization & Clarity Well-structured, clear, and
neat presentation
4

3. 1.Title of the Experiment
- Lab 1: Membrane Transport System
-
2.Purpose / Objective – What was the goal or concept of the experiment?
-The purpose of this lab was to understand how osmosis works. Osmosis is
the movement of water across a semi permeable membrane. We used an
egg to model a cell and observed what happens when it is placed in different
solutions like vinegar, corn syrup, and distilled water.
3.Summary of the Experiment – Briefly describe what was done and observed.
-We started by soaking a raw egg in vinegar for 94 hours 11 minutes and 4
seconds. The vinegar dissolved the shell, leaving the egg with just its
membrane, which is similar to a cell membrane. Then, we placed the egg in
corn syrup. 2 hours 35 minutes and 54 seconds, the egg shrunk because
water moved out of the egg and into the syrup. Lastly, we moved the egg into
distilled water and soak it for about 24 hours. This time, the egg swelled up as
water moved back into the egg
4.Expected Results – What results were expected or obtained?
-In vinegar, the shell dissolved because of a reaction with acetic acid. The egg
gets slightly bigger because water entered it. In corn syrup, the egg shrank. It
is because corn syrup is hypertonic it has more solutes, the resulted the water
to move out of the egg. In distilled water, the egg grew its size. Since, distilled
water is hypotonic has less solutes, so the result is the water moved into the
egg.
-

4. 5.Reflection and Interpretation – What did you learn or realize from this
experiment? How does it relate to biochemistry concepts and real-life
applications (e.g., health sciences)?
-From this experiment, I learned how osmosis works in cells. Water moves
depending on where there is more or less solute. If there is more solute. If
more solute outside, water leaves the cell. If more water outside, water enters
the cell. This is important in real life, especially in health and medicine. For
example, IV fluids must have the right concentration so red blood cells do not
burst or shrink. Because, if the IV fluid is too dilute or hypotonic, the cells can
swell too much and even burst. This dangerous in the brain since it can cause
headaches, seizures, or coma called hyponatremia.
-If the IV fluid is too concentrated, water moves out of the cell into the
bloodstream. Cells shrink or become dehydrated and it can lead to confusion,
muscle weakness, or dehydration. So, IV fluids should be balance or isotonic,
so that the water movement is equal and your cells will stay in normal size
and can do their job properly and healthy, and our body stays in homeostasis.