Bioluminescence.pptx

PG AND RESEARCH DEPARTMENT OF MICROBIOLOGY ,SRI PARAMAKALYANI COLLEGE, ALWARKURICHI.

1. SRI PARAMAKALYANI COLLEGE REACCREDITED WITH B GRADE WITH A CGPA OF 2.71 IN THE SECOND CYCLE OF NAAC AFFILIATED TO MANOMANIUM SUNDARANAR UNIVERSITY, TIRUNELVELI. ALWARKURICHI 627 412, TAMIL NADU, INDIA POST GRADUATE & RESEARCH CENTRE DEPARTMENT OF MICROBIOLOGY (Government Aided) ACADEMIC YEAR 2021-2022 II sem core: - microbial physiology – ( ZMBM13 ) BIOLUMINESCENCE Submitted by, A.MANI BHARATHI Reg no:20211232516113 I M.Sc microbiology Submitted to Guide: Dr.s.viswanathan Assistant professor & head.

2. SYNOPSIS INTRODUCTION BIOLUMINESCENT BACTERIA STRUCTURE CHEMICAL REACTION OF LUCIFERIN TYPES USES ADVANTAGES DISADVANTAGES REFERENCE

3. Bioluminescence Bioluminescence is the production and emission of light by the living organism. It is the form of Chemiluminescence . Enzyme and Light emitting molecule which are responsible for the emission of light are Luciferase and Luciferin

4. BIOLUMINESCENT BACTERIA Bioluminescent bacteria  are  light-producing   bacteria  that are predominantly present in sea water, marine sediments, the surface of decomposing fish and in the gut of marine animals. While not as common, bacterial bioluminescence is also found in terrestrial and freshwater bacteria. [1]  These bacteria may be free living (such as  Vibrio harveyi ) or in symbiosis with animals such as the  Hawaiian Bobtail squid  ( Aliivibrio fischeri ) or terrestrial  nematodes  ( Photorhabdus luminescens ). The host organisms provide these bacteria a safe home and sufficient nutrition. In exchange, the hosts use the light produced by the bacteria for camouflage, prey and/or mate attraction. Bioluminescent bacteria have evolved symbiotic relationships with other organisms in which both participants benefit close to equally. [2]  Another possible reason bacteria use luminescence reaction is for  quorum sensing , an ability to regulate gene expression in response to bacterial cell density. [3]

5. Structure of Luciferin: 4,5-Dihydro-2-(6-hydroxy-2-benzothiazolyl)-4-thiazolecarboxylic acid 

6. Chemical Reaction Bioluminescence is a form of Chemiluminescence where the light energy is released by a chemical reaction Luciferase Luciferin + O 2 Oxyluciferin + Light energy other cofactors

7. Types of Luciferin There are various types of Luciferin based on the presence in various organisms Coelenterazine Firefly luciferin Latia luciferin Bacteria Luciferin Dinoflagellates luciferin Vargulin luciferin Fungi Luciferin

8. Uses of bioluminescence Detect microbial load in raw milk (cfu/ml). Assess microbiological quality of beef and pork carcasses and minced meat (cfu/g). Monitor microbiological activity in indoor air (cfu/ml). Monitor sanitary conditions in clinical settings. Monitor yeast and bacteria in beverages and fruit juices. Monitor cleanliness (bio-burden) of NASA spacecraft (to limit terrestrial microbes being transferred to other planetary bodies) and to detect life (living cells) on other planets. Monitor water quality. Verify cleaning (whether equipment is clean enough to go for production).

9. Advantages Counterillumination camouflage Mimicry of other animals To attract other individuals for mating To attract other species for Prey Defence Communication

10. Disadvantage It does not easily distinguish ATP from microorganisms, animals, and plants. Luminescence from food can affect the actual ATP bioluminescence readings. The presence of detergents, sanitizers, or other chemicals also can affect the readings. It is not very sensitive for spore detection since the level of ATP is very low in spores. It does not substitute using traditional microbiological analysis.

11. REFERENCE https://www.qualityassurancemag.com/article/aib1013-atp-bioluminescence-assay/ https://en.m.wikipedia.org/wiki/Bioluminescent_bacteria#:~:text=Bioluminescent%20bacteria%20are%20light-producing,in%20terrestrial%20and%20freshwater%20bacteria .

12. THANKYOU The chairman. The Secretary. Management committee. The Principal,Sri paramakalyani college. The head – department of microbiology The staff members – department of microbiology