Biosafety cabinets[1].pptx Biological safety cabinets are important part of laboratory work

Biological safety cabinets are important part of laboratory work
FATHIMA RAFNA OP

1. Biosafety cabinets

2. Biosafety cabinets Biosafety cabinets are primary devices intended to minimize exposure when working with biohazardous materials These are designed to protect laboratory personal against exposure during experimental procedures (personal protection ), protect experimental materials from contamination (product protection ) and environmental protection

3. It is an acronym for "high efficiency particulate air [filter]" . This type of air filter can theoretically remove at least 99.97% of dust, pollen, mold , bacteria, and any airborne particles with a size of 0.3 microns (µm). HEPA FILTERS

4. Class I biosafety cabinet Most basic biosafety cabinet Provides personal and environmental protection No product protection as the unsterilized room air is drawn over the work surface Designed for general microbiology research The cabinets are open fronted They have walls, glass upper front

5. The unfiltered air is drawn through the front access opening It is a negative ventilated cabinet to draw adequate room air into the cabinet The air inside the cabinet then takes in the aerosol particles that may have been generated and moves it away from the operator towards the HEPA filter The air moving out of the cabinet is sterilized by HEPA filters before it is discharged to the environment In this way, the cabinets protect the operator and the environment from the aerosol but no the product .

6. A) Front opening B) Sash C) Exhaust HEPA filter Uses – procedures such as shaking, mixing of any material likely to contain dangerous organisms

7. Class II biosafety cabinet Provides personal, product and environmental protection These are open fronted cabinet It is a negative ventilated cabinet Working Air is pulled from the perforated front grill, that is, the dirty air is pulled from the operator side and thus providing safety to the operator The contaminated air is then pushed below the work station and taken upwards to the plenum by the blower or motor

8. From the plenum, the air is passed through the HEPA filter Usually 30% of the air is sent out to the environment through the HEPA filter Remaining 70% of the air passes through another HEPA filter and enters the working area of the cabinet Plenum Blower or motor

9. Class II BSCs are further divided into 5 types depending on the exhaust system and mechanism of work Type A1, Type A2, Type B1, Type B2, Type C1

10. Type A1 Internal fan – draws room air -75 fpm Air either recirculated after passing through the HEPA filters or exhausted out of the cabinet also through a HEPA filter 30% exhausted 70% recirculated Not widely used Not safe to work with hazardous chemical substances

11. Type A2 Internal fan – draws room air -100 fpm Approximately 60 to 70% of the contaminated air is recycled and pushed back into the work station in the chamber Remaining 30-40% is exhausted through the exhaust HEPA filter Not used extensively because of the chances of releases of hazardous chemicals into the environment

12. Type B1 Type B1 cabinets divide the airflow so that the contaminated air is directed towards the exhaust system while the air between the operator and work station mixes with the inflow and is recirculated 40% air recirculated 60% exhausted outside the room

13. Type B2 100% air is exhausted through duct system Advantages – removal of toxic vapours that are generated in the cabinet with no recirculation within the BSC Expensive Limited to toxicology laboratories

14. Working Mechanism of Class III Biosafety Cabine t Under pressure, this type of cabinet is examined to ensure that no particles can escape and enter the space. HEPA filters are used to filter the supply air, and two HEPA filters are used to release the exhaust air into the atmosphere. CLASS 3 BIOLOGICAL SAFETY CABINET

15. These metal-welded gas-tight chambers have heavy-duty rubber glove apertures in the front cabinet used to operate the work. The work surface can be expanded by connecting several glove boxes. The cabinet’s interior is kept under negative pressure by a dedicated exhaust system located outside the cabinet.

16. A double-door autoclave that decontaminates all materials entering or leaving the Class III cabinet may be attached to it. The Class III BSCs must have an associated pass-through box or dunk tank that can be sterilized and has an exhaust system with a HEPA filter.

17. Provide maximum protection to the operator, the environment and the sample against the high risk group 4 pathogenic organisms

18. Uses of Class III Biosafety Cabinet Weighing and dilution of chemicals that cause cancer. To investigate the risky agents To research emerging diseases. To research diseases that are almost completely eradicated To handle dangerous or highly contagious experimental materials

19. Biological Safety Cabinets Operating Procedures Prepare Work Area Switch off the UV light after 30 minutes of sterilization Switch on the blower and check the air grilles for blockages.
Allow air to purge workspace 5-10 minutes Pre-disinfection Apply the appropriate disinfectant to all interior surfaces by spraying or wiping
Allow air-dry

20. Assembly of materials and equipment Only include the materials necessary to carry out the process.
Set up the materials such that they don’t come into contact with any tainted ones.
Position the container for hazardous materials at the right rear. Pre-purge cabinet When there is no activity inside, let the air purge. Self-preparation Wear protective clothing, gloves, mask, etc.

21. Do the procedures Once operations are finished, and all necessary materials are secured, do not remove your hands from the work area. Remove your gloves before handling any contaminated materials. Post-purge cabinet When there is no activity inside, let the air purge. Finish personally Take off your protective clothing, and mask, and wash your hands

22. Post-disinfection Remove gloves and materials, place them in a biohazard bag, put them in an autoclave if necessary, and disinfect all internal surfaces with the proper cleaner. Shut down cabinet Turn off the fan and the fluorescent light, then on the UV lamp