biotech-regenration of plants, pharmaceutical applications.pptx

Biotech
M pharma 2nd send'
dr harisingh gour sagar university

1. Regeneration of Plants. Pharmaceutical Applications of Plant Tissue Culture.

2. REGENERATION OF PLANTS. PHARMACEUTICAL APPLICATIONS OF PLANT TISSUE CULTURE Assignment of PHARMACEUTICAL BIOTECHNOLOGY PHS C C 1203 Session 20 23-2024 Supervisors: PROF. UMESH K. PATIL DR. UDITA AGRAWAL DR.PRIYANKA JAIN MR.SATYAM SHYAMVISHWAKARMA Submitted by: ARYA OJHA Y 23254025 Department of Pharmaceutical Sciences Dr. Harisingh Gour Vishwavidyalaya, Sagar, (M.P.) (A Central University)

3. Table Of Contents 01 Introduction 02 Background 03 Types 04 F low chart 05 Discussion 06 References

4. ACKNOWLEGEMENT Throughout my assignment, I truly appreciate the help and encouragement I received from my guide and the following faculty members: PROF. UMESH K. PATIL, DR. UDITA AGRAWAL, DR. PRIYANKA JAIN, and MR. SATYAM SHYAM VISHWAKARMA.

5. W hat is regeneration? A natural process of restoring or replacing damaged, missed cell, tissue, organ and even entire body part in plants and animals is known as REGENERATION. Regeneration in plants: T he callus tissue growing on tissue culture medium undergoes redifferentiation into tissue systems and organs, followed by development into a new plant. T his process is called as plant regeneration. 1. INTRODUCTION

6. Differentiation: The process by which a cell loses its ability to divide and attains a specific function is known as differentiation. Meristematic cells differentiate to form permanent tissues. Dedifferentiation: The process by which a cell once again regains the ability to divide and lose the function it attained after differentiation is called dedifferentiation. Redifferentiation: The process by which a dedifferentiated cell attains a particular function and loses its ability to divide again is called redifferentiation Differentiated tissues are root, leaves , stems. Undifferentiated tissues are totipotent.

7. Physiology of plant regeneration: Meristem cell E.g.: shoot apical meristem Differentiated cell E.g.: leaf Regenerated new cell, cell type, organ Modified ratio of Auxin: Cytokinin ORGANOGENESIS SOMATOGENESIS

8. Apical/ terminal bud 2. Organogenesis Process of initiation and development of an organ like root, shoot, flowers is called organogenesis.

9. It is a process by which a totipotent cell undergoes embryogenic pathway to form somatic embryos which are grown to regenerate a new plant. Embryonic pathway? 3. Somatogenesis Male gamete(n) Female gamete(n) Zygote(2n) Zygotic embryo Somatic cell (2n) Embryo HOW??

10. In this embryo like structures are produced. The embryo produced is called embryoid. Studies have indicated that not only egg cell, but any cell of embryo sac can generate an embryo. Applications: In production of artificial seed Disease free plant production Less time taking Large scale production

11. Background Current landscape Highlight the current challenges and limitations in the medical field that require a breakthrough. Describe the healthcare problems, diseases or conditions that need innovative solutions Emerging trends Focus on emerging medical trends, technologies, or scientific discoveries that present an opportunity for a breakthrough. Discuss the potential benefits, such as more accurate diagnostics or preventive measures Breakthrough vision Outline a visionary goal for the breakthrough, such as curing a disease or revolutionizing procedures. Highlight the broader impact on healthcare and global quality of life

12. PHARMACEUTICAL APPLICATIONS OF PLANT TISSUE CULTURE INDOLE ALKALOIDS Ajmalicine, vincristine, vinblastine TROPANE ALKALOIDS Atropine, cocaine, scopolamine QUINOLINE ALKALOIDS Quinidine, Quinine   Production of phytopharmaceuticals and secondary metabolites. b)   Extracts from black  carrot tissue culture as potent anticancer agents. c) Berberine used as an oral medication for the treatment of high cholesterol, high blood pressure, diabetes, or high levels of lipids in the blood. The C. japonica was identified as having the highest levels of berberine, and today the plant material is being produced via tissue culture.

13. d) To study plant disease and their elimination e) In taxol production: enhancement of taxane production in hairy roots of Taxus * media var.Hicksii f) Micropropagation: for research and commercialization g) Embryo rescue h) Study of respiration and metabolism - study of respiration of whole callus, cellular fracture helps in separating normal and diseased cell i )  Various groups and establishments are already employing tissue culture in gene selection, and the propagation of Valerianaceae ( antitumor and cytotoxicity activities. ) j) Production of artificial seeds k) Somaclonal modifications

14. 1. Vyas S.P, Dixit V.P, “Pharmaceutical biotechnology”, CBS publishers and distributors, 1 st edition, 1998, Pg no.225-273 2. Murashige T. and Skoog F. (1962). A revised medium for rapid growth and bioassay with Tobacco tissue culture. Physiologia plantarum 15: 473-497. 3.   Chawala H.S,“Introduction to Plant Biotechnology”, Oxford & IBH Publishing C./ Pvt. Ltd, 2 nd edition, 2002, Pg.no. 555-590 4. Gad S.C, “Handbook of pharmaceutical biotechnology”, Wiley publications, 2007, Pg.no.691-698 5. Dagla , H. “ Plant tissue culture”, Resonance,17. 10.1007/s12045-012-0086-8. REFERENCES: