Body fluids and blood.pptx

Unit III - Body fluids and blood.
Body fluids, composition and functions of blood, hemopoeisis

1. Body fluids and blood By- Samruddhi S. Khonde Asst. Prof P. R. Patil Institute of Pharmacy. Talegaon UNIT III

2. Body fluids refer to the various liquids or semi-liquids that are present in the human body. These fluids play crucial roles in maintaining the body's homeostasis, transporting nutrients and waste products, regulating temperature, and supporting various physiological functions. The major types of body fluids include blood, lymph, cerebrospinal fluid, synovial fluid, saliva, gastric juices, bile, sweat, and urine. Blood: Composition: Blood is a connective tissue composed of plasma, red blood cells (erythrocytes), white blood cells (leukocytes), and platelets. Plasma is the liquid component of blood, and it contains water, electrolytes, proteins, hormones, and waste products. Function: Blood carries oxygen and nutrients to cells, removes waste products, transports hormones, and plays a crucial role in the immune system. Lymph: Composition: Lymph is a clear fluid that resembles plasma but lacks red blood cells. It contains white blood cells, proteins, and fats. Function: Lymphatic fluid is part of the immune system, helping to fight infections and diseases. It also plays a role in maintaining fluid balance in tissues. Body fluids

3. BODY FLUIDS Cerebrospinal Fluid: Location: Found in the brain and spinal cord within the central nervous system. Composition: Cerebrospinal fluid is a clear, colorless fluid that contains water, electrolytes, glucose, and proteins. Function: It acts as a cushion, protecting the brain and spinal cord from injury. It also helps transport nutrients and waste products. Synovial Fluid: Location: Found in joints. Composition: Synovial fluid is a viscous, lubricating fluid that contains water, electrolytes, and proteins. Function: It lubricates the joints, reducing friction between bones and facilitating smooth movement. Saliva: Location: Produced by salivary glands in the mouth. Composition: Saliva is a mixture of water, enzymes, mucus, and electrolytes. Function: It helps in the digestion of food, moistens the mouth, and has antimicrobial properties. Gastric Juices: Location: Found in the stomach. Composition: Gastric juices contain hydrochloric acid, enzymes, mucus, and water. Function: These fluids aid in the digestion of food in the stomach. Bile: Location: Produced by the liver and stored in the gallbladder. Composition: Bile is a digestive fluid containing bile salts, pigments, cholesterol, and water. Function: Bile aids in the digestion and absorption of fats.

4. Blood – it’s Composition and Functions Blood may be described as a specialized liquid connective tissue that consists of cells surrounded by a liquid extracellular matrix. The extracellular matrix is called blood plasma, and it suspends various cells and cell fragments The specific gravity of whole blood varies from 1.055 to 1.060. The freshly shed, blood is red, thick, opaque and slightly alkaline. Blood is denser and more viscous (thicker) than water and feels slightly sticky. The temperature of blood is 38°C (100.4°F), about 1°C higher than oral or rectal body temperature, and it has a slightly alkaline pH ranging from 7.35 to 7.45 (average = 7.4). The color of blood varies with its oxygen content. When saturated with oxygen, it is bright red. When unsaturated with oxygen, it is dark red. Blood constitutes about 20% of extracellular fluid, amounting to 8% of the total body mass. The blood volume is 5 to 6 liters (1.5 gal) in an average-sized adult male and 4 to 5 liters (1.2 gal) in an average-sized adult female. The gender difference in volume is due to differences in body size.

5. FUNCTIONS OF BLOOD Blood has three general functions: 1. Transportation. Blood carries carbon dioxide from body cells back to the lungs for expiration and oxygen from the lungs to the body's cells. It transports hormones from endocrine glands to other body cells and nutrients from the digestive system to other body cells. Additionally, blood carries heat and waste materials to different organs so they can be expelled from the body. 2. Regulation. All bodily fluids are kept in a state of equilibrium when blood is flowing. The water in blood plasma has cooling and heat-absorbing qualities, and its varying rate of flow through the skin—where extra heat can escape from the body—helps regulate body temperature as well. Additionally, the water content of cells is influenced by blood osmotic pressure, primarily through interactions of dissolved. 3. Protection. Blood can clot (become gel-like), which protects against its excessive loss from the cardiovascular system after an injury. In addition, its white blood cells protect against disease by carrying on phagocytosis. Several types of blood proteins, including antibodies, interferons, and complement, help protect against disease in a variety of ways.

6. COMPOSITION OF BLOOD Blood is a highly complex fluid which is composed of two parts-a liquid, called the plasma and different types of cells which remain suspended in the plasma. The cells are called the blood corpuscles. The plasma constitutes about 55%, and the cells about 45% of the total volume of human blood. The blood is made up of more than 10 different cell types. Each of these cell types falls into one of three broad categories: 1. Red blood cells (erythrocytes) 2. White blood cells (leukocytes) 3. Platelets (thrombocytes) The general composition of the whole blood is as follows- Whole blood cells : (a) Red blood corpuscles or erythrocytes (RBC). (b) White blood corpuscles or leucocytes (WBC). (c) Platelets or thrombocytes. Plasma : (a) Water, 91 to 92%, (b) Solids, 8 to 9%. Inorganic constituents : 0.9% Sodium, Potassium, Calcium, Magnésium, Phosphorus , Iron , Copper .

7. Organic constituents Proteins: 7.5% serum albumin, serum globulin, fibrinogen, prothrombin, etc. Non- protein nitrogenous (NPN) substances: Urea , uric acid, xanthine, hypoxanthine, creatine, creatinine, ammonia, amino acids, etc. Carbohydrate: Glucose, etc. Fats: Neutral fat, phospholipid, cholesterol, cholesterides , etc. Other substances: Internal secretions, antibodies and various enzymes (amylases, proteases, lipases, phosphatases, etc.). Colouring matter: The yellow colour of plasma is due to small amounts of bilirubin, carotene and xanthophyllin .

8. 1. Red blood cells (erythrocytes) Erythrocytes are small, biconcave disc-shaped cells without a nucleus in humans (nucleated in some animals). The biconcave shape provides a large surface area for the efficient exchange of gases and allows the cells to be flexible, facilitating their movement through narrow capillaries. The primary function of red blood cells is to transport oxygen, and they achieve this through the presence of hemoglobin , a protein that binds to oxygen in the lungs and releases it in the tissues. Hemoglobin also plays a role in carrying some carbon dioxide back to the lungs. Erythrocytes are produced in the bone marrow through a process called erythropoiesis. The production is regulated by the hormone erythropoietin, which is released by the kidneys in response to low oxygen levels in the blood. Red blood cells have a finite lifespan, typically around 120 days in circulation. After this period, they are removed from the bloodstream by the spleen and liver, and new red blood cells are continually produced to replace them. The characteristic red color of blood comes from the iron-containing hemoglobin molecules within red blood cells.

9. Erythropoiesis (Development)

10. Lifespan of Erythrocytes

11. White blood corpuscles or leucocytes (WBC). White blood cells, also known as leukocytes , are an essential component of the immune system. Their primary function is to defend the body against infectious diseases and foreign substances . These cells play a crucial role in identifying and eliminating pathogens, such as bacteria, viruses, and fungi, through various mechanisms like phagocytosis or the production of antibodies. Additionally, white blood cells also contribute to the regulation of inflammation and help in repairing damaged tissues . White blood cells, or leukocytes, are vital for the proper functioning of the immune system. They act as the body's defense mechanism by detecting and destroying harmful pathogens, preventing the spread of infection. Without white blood cells, the body would be more susceptible to diseases and unable to effectively fight off infections. There are several types of white blood cells, each with their own specific functions in the immune system. These include neutrophils, which are the most abundant and quickly respond to infections, as well as lymphocytes , which play a key role in recognizing and destroying infected cells. monocytes help to engulf and remove dead or damaged cells, eosinophils and basophils are involved in allergic reactions and inflammation.

12. Neutrophils are the most abundant type of white blood cell and are responsible for quickly responding to infections. They are often the first cells to arrive at the site of an infection and release chemicals that help to kill bacteria. Neutrophils have a short lifespan and are constantly being produced in the bone marrow to replenish their numbers. Monocytes are large white blood cells that can differentiate into macrophages, are the most abundant type of white blood cell and are responsible for quickly responding to infections. They are often the first cells to arrive at the site of an infection and release chemicals that help to kill bacteria. Neutrophils have a short lifespan and are constantly being produced in the bone marrow to replenish their numbers. Eosinophils primarily function in dealing with parasitic infections and allergic reactions. Eosinophils have a bilobed nucleus and cytoplasmic granules that stain with acidic dyes, giving them a distinctive red or pink color . The granules in eosinophils contain enzymes and proteins, such as major basic protein, eosinophil peroxidase, and eosinophil cationic protein. Role in Immune Response: Parasitic Infections: Eosinophils are particularly effective against parasitic infections, as they release toxic substances that can destroy the parasites. Allergic Reactions: Eosinophils are also involved in allergic responses, especially in the late phase. They release substances that modulate the inflammatory response.

13. Basophils: Basophils are involved in the inflammatory response and play a role in allergic reactions. Basophils have a bilobed nucleus, but their cytoplasmic granules stain with basic dyes, giving them a deep blue or purple colour . Basophil granules contain histamine, heparin, and various other chemical mediators. Role in Immune Response: Inflammation: Basophils release histamine and other substances that promote inflammation. Histamine increases blood vessel permeability, allowing immune cells to reach the site of infection or injury. Allergic Reactions: Basophils are involved in immediate hypersensitivity reactions. When triggered by an allergen, they release histamine, contributing to symptoms such as itching and swelling.

14. Lymphocytes are another type of white blood cell that are crucial for the immune response. There are two main types of lymphocytes: B cells and T cells. B cells produce antibodies that can neutralize pathogens, while T cells directly attack infected cells. These cells work together to provide long-term immunity against specific pathogens. The development and maturation of T cells takes place in the thymus, while B cells mature in the bone marrow. Once matured, both types of lymphocytes circulate throughout the body, constantly scanning for foreign invaders. - T cells play a crucial role in cell-mediated immunity by recognizing and destroying infected or abnormal cells, such as cancer cells. T cells mature in the thymus, a small organ located near the heart. There are several subtypes of T cells, each with distinct functions. Some of the key types include: Helper T Cells: These cells assist other immune cells by releasing signals (cytokines) that activate B cells and cytotoxic T cells. They play a central role in coordinating the immune response. Cytotoxic T Cells: These cells directly attack and destroy infected or abnormal cells, such as cells infected by viruses or cancer cells. Regulatory T Cells: These cells help regulate the immune response, preventing excessive reactions that could harm the body's own tissues.

15. B Cells (B Lymphocytes): B cells mature in the bone marrow. When B cells encounter specific pathogens, they can differentiate into plasma cells, which produce antibodies. Antibodies are proteins that can bind to and neutralize pathogens, marking them for destruction by other immune cells. Natural Killer (NK) Cells: NK cells are part of the innate immune system and do not require prior exposure to specific pathogens. They are responsible for recognizing and destroying infected or abnormal cells, such as those infected by viruses or cancer cells.

16. Platelets ( thrombocytes ) Platelets, also known as thrombocytes, are small, disc-shaped cell fragments found in the blood. They play a crucial role in the process of blood clotting, which is essential for preventing excessive bleeding when a blood vessel is injured. Platelets are one of the three main types of blood cells, along with red blood cells and white blood cells. Platelets are formed in the bone marrow through a process called thrombopoiesis. Megakaryocytes, which are large cells found in the bone marrow, undergo fragmentation to produce platelets. A single megakaryocyte can give rise to thousands of platelets. Platelets are tiny cell fragments, much smaller than red or white blood cells, with a diameter ranging from 1 to 3 micrometers . They lack a nucleus but contain cellular organelles such as mitochondria and granules. The granules in platelets contain various substances that are released during the clotting process. Function: The primary function of platelets is to prevent bleeding by forming blood clots. When a blood vessel is injured, platelets adhere to the site of injury and become activated. Activated platelets release chemicals that make nearby platelets sticky, causing them to adhere to each other and the damaged area of the blood vessel. This forms a temporary plug that helps to seal the breach in the vessel wall.

17. HEMOPOEISIS Simply, hematopoiesis is the  process through which the body manufactures blood cells . It begins early in the  development of an embryo , well before birth, and continues for the life of an individual. It occurs within the hematopoietic system, which includes organs and tissues such as the bone marrow, liver, and spleen. All blood cells originate from pluripotent stem cells and go through several developmental stages before entering the blood. Different types of blood cells follow separate lines of development. The process of blood cell formation is called haemopoiesis takes place within red bone marrow. Hematopoiesis   begins during the first weeks of embryonic development . Before birth, hemopoiesis first occurs in the yolk sac of an embryo and later in the liver, spleen, thymus, and lymph nodes of a fetus . Red bone marrow becomes the primary site of hemopoiesis in the last 3 months before birth, and continues as the source of blood cells after birth and throughout life. Red bone marrow is a vascularized connective tissue found in the axial skeleton, pectoral and pelvic girdles, and proximal epiphyses of the humerus and femur.

18. About 0.05–0.1% of red bone marrow cells are called pluripotent stem cells. or hemocytoblasts and are derived from mesenchyme (tissue from which almost all connective tissues develop) . These cells have the capacity to develop into many different types of cells. In order to form blood cells, pluripotent stem cells in red bone marrow produce two further types of stem cells, which have the capacity to develop into several types of cells. These stem cells are called myeloid stem cells and lymphoid stem cells . Myeloid stem cells begin their development in red bone marrow and give rise to red blood cells, platelets, monocytes, neutrophils, eosinophils, basophils, and mast cells. Lymphoid stem cells, which give rise to lymphocytes, begin their development in red bone marrow but complete it in lymphatic tissues Lymphoid stem cells also give rise to natural killer (NK) cells. Although the various stem cells have distinctive cell identity markers in their plasma membranes, they cannot be distinguished histologically and resemble lymphocytes. During hemopoiesis, some of the myeloid stem cells differentiate into progenitor cells ( prō -JEN- i -tor). Other myeloid stem cells and the lymphoid stem cells develop directly into precursor cells (described shortly). Progenitor cells are no longer capable of reproducing themselves and are committed to giving rise to more specific elements of blood. Some progenitor cells are known as colony-forming units (CFUs).

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