Bt Cotton

Bt Cotton and
Its Use and Genetic Development

1. Bt Cotton : An Introduction Benefits and Development Procedure Presented By: Shahida Bano Junior Research Analyst, (Agro-commodities-Cotton)

2. From Bacteria to Bio-pesticide Bt cotton is a GM , Genetically Modified crop, is developed to reduce the losses arises due to pest attacks Genes of Bt is used to develop the Bt cotton with the help of advance techniques genetic engineering

3. Bt cotton is the discovery to combat the effects of two most injurious caterpillar pests

4. Contribution of Bt in Cotton When insects eats this Cry-protein, its own digestive enzyme stimulates a toxic protein Cry-Proteins binds to specific receptors and ruptures the mid gut cells Susceptible insects stops feeding and dies within 2-3 days

5. Agrobacterium tumefaciens Plasmid DNA Plasmid DNA removed and cut open using restriction enzyme Gene of interest identified on chromosomal DNA of the Bacteria Gene removed using the restriction enzyme Re inserted the plasmid into Agrobacterium Every daughter cells gets the same genes when cells divides Transferring the genes to the Chromosomal DNA of Plant Cell Each daughter cell receive the trait when plant cell divides Using Plant tissue culture technique to grow plant from the Genetically modified cells Genetically Modified Plant With Desired Traits Recombinant Plasmid

6. Thank You