Cell Transport PowerPoint, including active transport, passive transport and bulk transport

This presentation contains information about cell transport including specifically passive transport and the types. It also includes active transport and the types. In addition to these it includes a short section about bulk transport that uses white blood cells as an example

1. Cellular Transport Notes

2. About Cell Membranes All cells have a cell membrane Functions : Controls what enters and exits the cell to maintain an internal balance called homeostasis Provides protection and support for the cell TEM picture of a real cell membrane.

3. Structure of cell membrane Lipid Bilayer -2 layers of phospholipids Phosphate head is polar ( hydrophilic or water loving) Fatty acid tails non-polar ( hydrophobic or water fearing) Proteins embedded in membrane About Cell Membranes (continued) Phospholipid Lipid Bilayer

4. Proteins Membrane movement animation Polar heads love water & dissolve. Non-polar tails hide from water . Carbohydrate cell markers Fluid Mosaic Model of the cell membrane

5. 4. Cell membranes have pores (holes) in it Selectively permeable : Allows some molecules in and keeps other molecules out The structure helps it be selective! About Cell Membranes (continued) Pores

6. 5. Size (macro or micro), charge (+ or -) and solubility (lipid-soluble or not) affect a molecule’s ability to cross the cell membrane. In general, small noncharged, lipid-soluble molecules easily cross the cell membranes About Cell Membranes (continued) Pores

7. Outside of cell Inside of cell (cytoplasm ) Lipid Bilayer Proteins Transport Protein Phospholipids Carbohydrate chains Structure of the Cell Membrane Go to Section: Animations of membrane structure

8. Types of Cellular Transport Passive Transport Active Transport Bulk Transport high low This is gonna be hard work!! high low Weeee!!! Animations of Active Transport & Passive Transport

9. Passive Transport cell uses no energy molecules move randomly Molecules spread out from an area of high concentration to an area of l ow concentration . (High Low)

10. 3 Types of Passive Transport Diffusion Facilitative Diffusion – diffusion with the help of transport proteins , Osmosis – diffusion of water

11. Passive Transport: 1. Diffusion Diffusion : random movement of particles from an area of high concentration to an area of low concentration . ( High to Low ) Diffusion continues until all molecules are evenly spaced ( equilibrium is reached)- Note: molecules will still move around but stay spread out. http://bio.winona.edu/berg/Free.htm Simple Diffusion Animation

12. Passive Transport: 1. Diffusion Occurs in liquid or gas medium Diffusion of a solute within a solvent is affected by several factors: Temperature Pressure Density of the solvent Concentration Gradient Solubility of the solute http://bio.winona.edu/berg/Free.htm

13. 2. Facilitated diffusion : diffusion of specific particles through membrane transport proteins to help them move through the cell membrane Transport Proteins are specific – they “select” only certain molecules to cross the membrane Transports larger or charged molecules Concentration gradient required – can’t transport from low to high concentration Energy not needed Rate of transport reaches a maximum when all membrane transport proteins are used up (saturated) Membrane transport proteins are sensitive to inhibitors that can cause them not to function Passive Transport : 2. Facilitated Diffusion http://bio.winona.edu/berg/Free.htm

14. There are 2 types of membrane transport proteins involved: Channel proteins – contain tunnels/openings that serve as passageways of molecules Carrier proteins – undergo temporary binding to the molecule it carries resulting in conformational change that moves the molecule through the membrane Facilitated diffusion (Channel Protein) Diffusion (Lipid Bilayer) Passive Transport : 2. Facilitated Diffusion Carrier Protein A B http://bio.winona.edu/berg/Free.htm

15. High Concentration Low Concentration Cell Membrane Glucose molecules Protein channel Passive Transport: 2. Facilitated Diffusion Go to Section: Transport Protein Through a  Cellular Transport From a- High Low Channel Proteins animations

16. 3. Osmosis : diffusion of water through a selectively permeable membrane Water moves freely through pores. Solute (green) to large to move across. Osmosis animation Passive Transport: 3. Osmosis

17. The water molecules will continue to cross the semi-permeable membrane until an equilibrium is reached, where the two solutions are of equal concentration.

18. Water is found inside and outside of the cell, separated by a semi-permeable membrane The presence of different solutes in the water solutions in and out of the cell means concentration of water on both sides is different. Water Potential – term for movement of water molecules as it undergoes osmosis - The measure of the difference between the “force” that pushes water molecules and the “force” exerted by the membrane Passive Transport: 3. Osmosis

19. Water solution with less solute (more diluted solution because of more water) = higher water potential Water solution with more solute (more concentrated solution) = lower water potential Water Potential Gradient - when 2 solutions of different water potentials are separated by a semipermeable membrane, thus allowing osmosis to occur Osmotic Pressure – “force” that moves water molecules through a semi-permeable membrane Passive Transport: 3. Osmosis

20. Passive Transport: 3. Osmosis Tonicity – strength of a solution in relation to osmosis 3 Types: Isotonic Hypotonic Hypertonic Remember: Salt is a solute, when it is concentrated inside or outside the cell, it will draw the water in its direction. This is also why you get thirsty after eating something salty.

21. If the concentration of solute (salt) is equal on both sides, the water will move back in forth but it won't have any result on the overall amount of water on either side. " ISO " means the same

22. In this case there are less solute (salt) molecules outside the cell, since salt sucks, water will move into the cell, causing it to swell. The cell will gain water and grow larger. As osmosis continues, osmotic pressure builds up inside the cell causing cell lysis. " HYPO " means less In plant cells, the vacuoles will fill and the plant becomes stiff and rigid. The pressure exerted by the water molecule is called TURGOR PRESSURE , and the phenomenon TURGIDITY . The turgor pressure in plants will not result in cell lysis as the cell wall keeps the plant from bursting. TURGIDITY is important in maintaining the firm and erect position of a plant.

23. In this case there are more solute (salt) molecules outside the cell, which causes the water to be sucked in that direction. Causes cells to shrink and lose shape because of water loss. In plant cells, the central vacuole loses water and the cells shrink, causing wilting ( plasmolysis) . In animal cells, the cells also shrink. In both cases, the cell may die. " HYPER " means more

24. Active Transport cell uses energy (ATP or Adenosine Triphosphate) actively moves molecules to where they are needed Movement of molecules against concentration gradient (concentration gradient not required) Movement from an area of low concentration to an area of high concentration (Low  High) Transport proteins are highly specific Involves carrier proteins (like facilitated diffusion) often called pumps because they use energy to move molecules against a concentration gradient

25. Example: Sodium -Potassium Pumps are important in nerve responses. They transfer Na and K ions in and out of the nerve and muscle cells. Sodium Potassium Pumps (Active Transport using proteins) Protein changes shape to move molecules: this requires energy!

26. Types of Bulk Transport 1. Endocytosis : taking bulky material that can’t use transport proteins (like macromolecules) into a cell Uses energy Cell membrane in-folds around the macromolecule to be transported 3 Types: Phagocytosis Pinocytosis Receptor-mediated Endocytosis

27. Types of Bulk Transport Phagocytosis – “cell eating” Process by which cells take in large particles by infolding the cell membrane to form endocytotic vesicles Ex. WBC capture and kill invading bacteria

28. Types of Bulk Transport Pinocytosis – “cell drinking” Process of taking in fluids into the cell by invagination of the cell membrane. Any solute or small particles in the fluid will be moved into the cell.

29. Types of Bulk Transport Receptor-mediated Endocytosis – “cell drinking” Compared to pinocytosis, is very specific. The plasma membrane becomes indented and forms a pit. The pit lined with receptor proteins picks specific molecules from its surroundings. The pit will close and pinch off to form a vesicle which will carry the molecules inside the cytoplasm.

30. Types of Active Transport 2. Exocytosis : Forces material out of cell in bulk membrane surrounding the material fuses with cell membrane Cell changes shape – requires energy EX: Hormones or wastes released from cell Endocytosis & Exocytosis animations

31. Effects of Osmosis on Life Osmosis - diffusion of water through a selectively permeable membrane Water is so small and there is so much of it the cell can’t control it’s movement through the cell membrane.

32. Hypotonic Solution Hypotonic : The solution has a lower concentration of solutes and a higher concentration of water than inside the cell. (Low solute; High water) Result : Water moves from the solution to inside the cell): Cell Swells and bursts open ( cytolysis )! Osmosis Animations for isotonic, hypertonic, and hypotonic solutions

33. Hypertonic Solution Hypertonic : The solution has a higher concentration of solutes and a lower concentration of water than inside the cell . (High solute; Low water) Result : Water moves from inside the cell into the solution: Cell shrinks ( Plasmolysis )! Osmosis Animations for isotonic, hypertonic, and hypotonic solutions shrinks

34. Isotonic Solution Isotonic : The concentration of solutes in the solution is equal to the concentration of solutes inside the cell. Result : Water moves equally in both directions and the cell remains same size! (Dynamic Equilibrium) Osmosis Animations for isotonic, hypertonic, and hypotonic solutions

35. What type of solution are these cells in ? A C B Hypertonic Isotonic Hypotonic

36. How Organisms Deal with Osmotic Pressure Paramecium (protist) removing excess water video Bacteria and plants have cell walls that prevent them from over-expanding. In plants the pressure exerted on the cell wall is called tugor pressure. A protist like paramecium has contractile vacuoles that collect water flowing in and pump it out to prevent them from over-expanding. Salt water fish pump salt out of their specialized gills so they do not dehydrate. Animal cells are bathed in blood. Kidneys keep the blood isotonic by remove excess salt and water.

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