Cleaning of new glassware by rahul gautam

Cleaning of laboratory new glassware is not as simple as washing the dishes. Here is how to wash your lab glassware so that you will not waste your chemical solution or laboratory experiment as well as time consumed during the experiment.

1. CLEANING OF Laboratory NEW GLASSWARE By : Rahul Gautam M.Sc. Biochemistry Certified MLT & Phlebotomy Trainer

2. OBJECTIVES Principle Requirements Procedure

3. CLEANING OF LABORATORY NEW GLASSWARE Principle : Lab glassware is usually manufactured from borosilicate glass ( hard glass). It is resist to the action of chemicals with the exception of hydrofluoric acid and phosphoric acid. It can withstand mechanical breakage and sudden change of temperature . Soda lime glassware (soft glass) is not as good as borosilicate glassware.

4. REQUIREMENTS Soda lime glassware ( soft glassware) Borosilicate glassware or hard glassware. 5% HCL Tap water Distilled water 2% detergent solution

5. PROCEDURE FOR SODA LIME OR SOFT GLASSWARE The free alkali present on soda lime glassware is neutralized by treating overnight with 5% HCL

6. 2. Rinse the glassware several times in tap water .

7. 3. After rinsing with tap water rinse the glassware several times in distilled water .

8. 4. Dry the glassware in hot air oven

9. Note : To test that the free alkali has been neutralized , Autoclave the glassware in neutral distilled water and when cool check the pH of water.

10. THANK YOU