CLUSTER OF DIFFERENTIATION

M.Sc. MICROBIOLOGY (MEERUT INSTITUTE OF ENGINEERING AND TECHNOLOGY ).

IMMUNOLOGY.
CDs are cell surface molecules expressed on leulocytes and other cells.
CDs acts often as ligands or receptors (the molecule that activates the receptors) important to cell. a single cascade is usually initiated , altering the behaviour of cell.
some CDs protien do not plaay role in cell signaling , but have oher functions , such as cell adhesion .
there are approximately 250different proteins.
reactivity of most common antibodies (CD MARKERS)used in flow cymetric evaluatin of hematolymphoid neoplasms.

1. ASSIGNMENT

Subject code : GM -302

Subject name: IMMUNOLOGY

Regarding : CLUSTER OF
DIFFERENTIATION

Class; M.Sc. Microbiology ||nd
year

submitted to

Ms, Nikita maam

submitted by

Anjali Sharma

2. INTRODUCTION

3. cpa

Ne Lysahocate

Etta
Helper
The
se FR _
= tm A
La ces Fr
Natural Kalen

4. Ruane Aus
à

‘ he o) inmune
components a

Me Lumune system omd other

alone, auch as the oia ales

du Human Heukocyte Tbe fterunttakinn Anktquns [Hepa]
Workikeps sure tattealrd oe Wing order to Mu chaos
Hat entlid Ju

3924, as Ammunclogiste qarenated

Jonge aude

s el auonbelenal ankthoddis duarte

sileouyte cell austere w

ed > each wtth

alu “a

hed Adabil eluate o atbodds

pattou a} nd Jurhocyles

hone the

5. Ms proutdal à commen vnemenclalore Mat ablewed

Au Actonlifi’e community ko communtcate auautte

Fx a entera Language

Abidin of atouinily and diles af the Ln
Austen hou ban queakty Jactlttatnd, over Je
task So yas by due HEbA Werbakept volta awe
mud aukthodits do chanracterthe wang 4 me
meet Ile Je tamunclegical proces,
and Max provided a mmuncatus Agiles Hoe
ED austen, which As sad tora

ED wotteuts are defied op A the
raferente menectonal aultbeddi de muhteh Muy
Ind. Shane au a stators ef Ananda bon,
man elle expan Woe Shan one CD maker.
waster, A phendgpte ehanackentzakéon ef a cell
4 hematepstekic ir war be made by
cramming dhe pattern of CD marks enpsced
ok omy quien tue. She phgtelegte punckon
har ben vidente fred dor marga but mat all,
Aneum De.

6. NOMENCLATURE

, CD antigens by differentiation cell

Key Key
Antigens- Antigens-
Human Human
cps
TCell gg CDA Monocyte cos
CDs
scot @ CD Granuocy “cea
co
cDiic
Dendrte € M GDS Platelet gy CD!
NK Cell 7 CD56 Erythrocyte @ CD235a
stemcen @ coo Endothelial à. CDi46

Macrophas #& CD14 _— Epithelial PP co326

7. e Nomenciarure

7 he Human tubkecyte Dfferunstakin Antiqua
(HL on) workshops am qropetsd and estabtished
stn Ake LS Ankennakbual Wokshap :
Wis ken was ctatended for Au elasstftcakten
AL she wang meno clomal ankkbodas Um Abs]
quad by Alferunk dador adeñes osnund Me
ad sqoüuk epitopes on Au aurfoce
moleutes 4 Aube cles + Stree then, Ste use
has orpiead expanded de many ether cell apes ,
and mou than 320 Cd untque cluster and
aubdusters hove bun tolentifrcd + Uhr proposed
PS amplecule to autqnd a CD number ones
wo specific menoclenal antibodies [mAb) aw
Ahown ko bind do she wolteuter + 8] the molicute
das nok lan all chanactertzcl 4 0 has enla
on mb, Zt Ja mauallg given Abe wontetmal

Thatcator wr [asta teen ree],

8. MARKERS

Type of cell markers
stem cells cD31-
all groups — CDAS

Granulocyte CD45+, CD15+
Monocyte CD45+, CDI4

T lymphocyte

T helper cell

CD3+, CD4+

; CD3

Cytotoxic T cell CDS+

B lymphocyte CD45+, CD19+ or CD45+,

9. *

es
CDs as Marker
ee)

Such molares

Afteronlakten Len} mare » She Cd claut fecatton
Wes eatabtished fa 39993 hile ont gtnalty
Menard, only for whike blood call marken, Ht
MAG Anos atmen expanded do wang eut dypess At
Boek count, own 350 CD antiques haus been Tdenléfied.
ME is dolo nad gach, cate ype has

Sr own a

web words thet con amu as
Vente daga, Mau dues CD wontons
8 4
aus helped phystetanr Jo undinakand sol
Baro La 40 mue vantahon among le déffennt
AYP a} Lymphoma
DAJeurd classes a All can Mad Le dtekéngutsal,
shower , by visu sl Are et Shot Mus express
unique cembtnattens +} mdleules Ln Aer membranes,
Que dissiotid as of dfeuvk cet types Tnvolued Ex
mms suapenala ue à Het rest eb Me
development 4 Hacks do Arskenqutch Altar
votes cells by Hair cell a marken , We

U ate Hat auch area include wolreuter

11. <ose

eva
cou
Shen epacs
el cod Chane
7 ) ®

HORS
; =H ee Peg 3 E) Beco Angers
© Granwoevre CD Ánrigens:
> Yu ape ype of January tes a mecchoplily
cosheplula. $ basoptils
D Posoplil exprese aval adhesion mel „ohtehplay
a enitical noe in touting , dretudtug ZEA 4
Ucpualevie] , Mac -1[co11b) nas] ep44.

e ImmunoruencTvetng :

DAproers Akt aras ardtbodtea vo Tdentify colla based on
da types 4 aligns or marke on He fac dive
CD, Chis process a ud to Wognare ap Xp
of Mukemío Aywpboma dattur cute of Fomine ysl

3a CDs mankers wilde) ud Amma; ts

: Ñ = Y Apr im pence
wu various wuhude heeding fiw guay

12. 2 Beco

3) Dende

Y NKcelt

) Skem Cell
6) Macrophage

1?) Monocyte
9) Gransbocyte

9) Phobetut

19 bypthoworyte
13) endothelial
1) égttheliol

CDs, cpa, cre
daa, cho
CDiie, cdI23

casé

cosa

Cors

GES

epee

Cai, cds , CD62
chasse
cor

cosas *

13. FUNCTIONS

T cell CD antigen. B cell CD antigen.
cour cour Eva cabe
028 e O, oz
SAO O, cos
co of do ove co al O
cos (Y Do cod) LT
N O rer le cl co29
cos OS © cos co22 © eos

wat ur e020 C2

14. © Funcrion :

he Austen A déf{entiahton [often ablsautated as
CD] Le à pretocal md fos athe Lente ft colton and
nvadtgalten af Al ausfecr mel peon,
Nangeta for Sumo phishing af elle Du dom
Puga co antiguas cam ost un moi
aware, often eating as nereptous er Mgandı Cie
moule dhat acktvater a suceptor] Important do
du cel. A atqual coseade de walls Juiltaled,

Rae
Moin Ihe behavior of Aue cod. Seme CD

ankgus do met play a role La cell sigue
ud Lau other juni 9 suchas Leit
Adlon , cell

acivalón and col Inhzbetion-
Yhe D as ore comento sud as cl makers
{

in anmunoplunotgpöng » Alertas, celte do le den
hosed ou what molculia ane pusent on thee
surface, Ua ql ore aad do
asoctole eus ati Immung Junctions + Asche
tn ene CD maleate do de Ropulottons ua
UNCOMMON 4 cd mark as Alomed yer

call Aypıs with vn peu frios SS

dame agite

15. “the
Ata

CD aystem a commonly arsed as cell mandos
L 4

allows cota do ba defined based en what

Melua ou present om dhein adore Cicas

reed markus ane often word do assoc ae cells

AR ceda tone Juncker oF propedter’

PK ang ene Cd moleute do dejas populations

fe uncommon [ Haugh à fens examples exist],
combining workers has allowed for co Sp

voile wog apect{ic olegenikiom auto die Immune

atpthean

Pacto vita ch Ake mort common ankthodis [ en

Markus) ad Eu Pew Cybmuhie Evaluator, +

Nemakolympkoid Neo plasma

16. CLINICAL
APPLICATIONS

17. x Clinical Applications © of CD ant CD antigen:
9 Araba à Mubaunda À Lymphoma:
> A ukaemt er fynplame will opus a x ate

Markos reto Sw ta «toga 2 posto of ag" la
ore Cut arcade.

En

9 Becken of minimal racial ë x

D. MRD was clefivad as dieran do ad the Mit of
Segtcal detect ag vue mieroscapy .

STokitds ait aude uk Gent were conatdoud tobe tn
sumarios sehen bore Marne dompler condiud sh

wep! neaptoakic cel, Hous cytomabte wethed cam
dket Jar Rewer uuls of din.

18. SS EUR ne RE à
3 Atem Celt Enumsralten

Sn He bore marco can be Adleutrfted

> Haamopatetic Atem celle

Sy Aker emp" a} eps

> Normally lie nar af andr celts Au boue musgos La few

and napitatiste ln ghentphexa blood -

Y Saus organ Wanplartation :

> Tc ass match 5 Slow gone can be wand Je enosmahch
o sutplenl's avui auf donor ego ploc gis.

> Analagats 4 pe pestpheral blend Lymphogster may Rep

Mo Mdtale easily rige”

5) dmmuno deffctenty Disease

a Moca roauditng from prt Ammunodafleteneter Can
le asusto ape, In Tell, reel,
gpamalocgtes or wumougtes

6) Hiv Inuetton,
D Determination of no of CdAlrve) Symphocybes In perttharcal
blend La used do monter palfeuls wilt tu ange".

3 Tu y 4 cha (ave) calls com be akt. Ana stage tube

bg aii fee cenas] cos [Cra

19. x Rererences

4) Bernard AR, chal. eds : huucocube tps Human
Areas Alkferıdiabion Antigens Detected by.
Monoconel antibodies + Renin: Springen ~ Verlag » 1194

2) Zola Weal. CD meleculos 2005 1 Human cell
difouuiation met + Blood Ass Sud 39 Doi

lo-182/ blood - Auos 03-1338

3) Zeta, ot. Cd meieuldos 2006 -human cell
AH ereutiation uateeutes *T> Immonet Methods 2007
ES

9) Retchank IM, Paula AE. More clonal modes market
Now Rex Drug Discov 24; 31 383- 4