comparison of light and electron microscopes

1. Comparing Light and Electron
Microscopes
Advantages of Electron
Microscopes
•Greater magnification up
to 1,000,000 x
•Greater depth of field
(With SEMs, 3-D )
•No complex lighting
•Greater resolution-1 nm
Disadvantages of Light
Microscope
•Magnification only up to
1000-1,500 x
•Less depth of field
•May involve complex
lighting requirements
•Less resolution - 1 micron

2. Light Microscopes

3. Light microscope images
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4. Microscopes Continued
Disadvantages of
Electron Microscope
•Expensive (1 million)
•Expensive to Run
•Image Black and
White
•Dead Specimen
•Requires Vacuum
•Expert Preparation
•Special Large Room
•Affected by Magnetic
Fields
Advantages of Light
Microscope
•Cheaper (100)
•Cheaper to Run
•Color image
•Live or Dead
Specimen
•No Vacuum Required
•Simple Preparation
•Small and Portable
•Unaffected by
Magnetic Fields

5. Electron Microscope images
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6. SEMs TEMs
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435x (SEM)
40 x (SEM)
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1540 x

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3080 x
Eye of a Mosquito

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1760 x