CPCSEA and IAEC guidelines for animal safety and experiment. it is a regulatory commieetee which improve animal care guidelines

This content helps to understand guidelines for animal safety in terms of their use in experimental work regarding institutional and research levels. This gives a brief introduction to animal care, housing facilities, and transportation.

1. CHHATRAPATI SHAHUJI MAHARAJ UNIVERSITY KANPUR SCHOOL OF PHARMACEUTICAL SCIENCES CPCSEA GUIDELINES FOR LABORATORY ANIMAL FACILITY PRESENTED BY: RIYA GUPTA M.PHARM FINAL YEAR (PHARMACEUTICS) SOPS, CSJM UNIVERSITY, KANPUR

2. CONTENT: INTRODUCTION IAEC FUNCTION & COMPOSITION GOALS & OBJECTIVE ANIMAL FACILITIES LAW & GUIDELINES TRANSPORT OF LAB ANIMAL

3. INTRODUCTION: CPCSEA C ommittee for the P urpose of C ontrol and S upervision of E xperiments on A nimals. Stautory formed by the act of the Indian parliament under the Prevention of Cruelty to Animals Act,1960. Formed in 1964 . Revived in 1998 , under the committed chairpersonship of Menka Gandhi. Headquarters at Chennai. National-level expert committee to scrutinize and approve the research projects conducted using animals. Introduced a national “ Good Laboratory Practices” document. Introduced the credo of 3R principals. 2004- CPCSEA officially accepts the concepts of the 4R This committee is composed of -

4. Member of the scientific community Regulatory authorities Animal activist The committee has 10 experts and 3 official members The role of CPCSEA is to monitor animal experiments through ethics committees set up in institutions. CPCSEA nominee- important link between CPCSEA & IAEC. IAEC scrutinizes all project proposals for experimentation on animals. For small animals: give the final approval For large animals: make the recommendation to sub-committee on large animals.

5. Goals and objectives Goals: To promote and human care of animals used in research. To provide specifications that will enhance animal’s well-being and quality of research. Objective: Effective Functioning of institutional animal ethical committee. Main Activities Registration of establishment for experiments on animal For breeding of animal Approval of animal house facilities Permission of committee for conducting experiments

6. IAEC composition and function This committee is constituted in registered laboratories under CPCSEA. As defined in “Breeding of and Experiments on Animals (Control and Supervision) rules 1998”. IAEC means a body comprising a group of persons recognized and registered by the CPCSEA, performed in an establishment which is constituted and operated in accordance with procedures specified for the purpose by the committee. The objective of IAEC is to contribute to the effective functioning of the institutional ethics committee so that standard and consistent ethical review procedures for animal research are practiced for all proposals dealt with by the committee as prescribed by the CPCSEA under the PCA Act 1960 and breeding and experimentation rules 1998. Composition: It included 8 members as follows A biological scientist Two scientists from different biological disciplines A veterinarian involved in the care of the animal Scientist in charge of the animal facility of the establishment concerned A scientist outside of the institute

7. 6. A non-scientific socially aware member 7. A nominee of CPCSEA The chairperson of the committee and member secretary would be nominated by the institution from among the eight members. Members against serial numbers 1-4 will be nominated by the institute and approved by CPCSEA whereas members 5-6 and 7v will be nominated by the CPCSEA. Function of IAEC: All research projects using small animals such as rats, mice, rabbits, or guinea pigs have to be approved by the IAEC before initiation. Every member of the IAEC has the right to question/disapprove of a project, or unapproved proposals and referred to a subcommittee of experts for scrutiny and possible approval IAEC can’t approve research projects on large animals such as dogs, goat, sheep, nonhuman primates cattle, and so forth and can only recommend the same to the sub-committee of the CPCSEA which alone has the power to approve projects on higher vertebrates .

8. Monite research throughout the study and after completion of the study through periodic reports and visits to animal houses and labs where the experiments are conducted. The committee has to ensure all regulatory requirements applicable rules guidance and laws. IAEC accords quality and consistency in reviewing research proposals and prevents the infliction of unnecessary pain and suffering before, during, and after experiments on animals, following the CPCESA guidelines. CPCSEA law and guidelines: Veterinary care: Provided by veterinarian Daily observation of animals adopted Quarantine, stabilization, and separation : Quarantine period for small lab animals: 1 week to 1 month Large lab animal: up to 6 weeks Physiologic, physiological, and nutritional stabilization required Physical separation of animals by species to prevent anxiety and behavioural changes.

9. Animal procurement and transportation Procurement of animals done from established commercial animal vendors. During transport, stress is avoided by: Use of transport containers (cages or crates) of appropriate size. Permissible number of animals accommodated in each container . Experimental area: It should be carried out in a separate area away from the place where they are housed. Separate functional areas for Surgical support Treatment of animals Post-operative and intensive care Physical facilities:

10. Building materials Corridor Animal room doors Exterior windows Floors Drains Storage areas Environment Temperature Humidity control Power lighting Noise control Durable, moisture-poof, fire resistant and pest resistant Wide enough to facilitate the movement of personnel and equipment Should fit properly, should not be rust Windows are not recommended Smooth, moisture proofs, non absorbent, skid-proof floors Proper drainage, floors should be sloped Designed for feed, breeding, cages, and materials not in use Between 18-29°C Range of 30-70% Designed with 12-15 air cycles/hr. System should be safe, fluorescent lights are efficient, and emergency backup needed Noise free

11. Animal husbandry Caging or Housing system Adequate space, freedom of movement, and normal postural adjustments. Comfortable environment Easy access to food and water Adequate ventilation Bedding Should be adsorbent, free from toxic chemicals Should be removed and replaced periodically with fresh materials Ideal to change the bedding twice a week

12. Sanitation and cleanliness Animal rooms, corridors, storage spaces, and other areas should be cleaned with appropriate detergents and disinfectants Wastes should be removed regularly and frequently Cages should be sanitized before animals are placed in them Proper maintenance of records must Should maintaining the SOPs Proper selection of animal facility staff required Anaesthesia should be complete and without any pain Euthanasia is done when an animal is required to be sacrificed or termination from an experiment or otherwise for ethical reasons

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