CPCSEA GUIDELINE .pptx

In this PPT we discuss about the CPCSEA guidelines for animals during research work

1. CPCSEA GUIDELINE Committee For The Purpose Of Control And Supervision Of Experiments On Animals. Prepared B y : Ankur Roll No : 113 M.PHARM (PQA) Subject : Quality Control And Quality Assurance DIPSAR

2. Contents: INTRODUCTION OBJECTIVES FUNTION VETERINARY CARE ANIMAL PROCUREMENT QURANTINE , STABILIZATION , SEPARATION TRANSPORT OF ANIMAL

3. INTRODUCTION The Committee for the Purpose of Control and Supervision of Experiments on Animals (CPCSEA) is a statutory body formed by the act of the Indian Parliament under the Prevention of cruelty to animals act 1960.

4. OBJECTIVES The goal of these guidelines is to promote the human care of animal used in biochemical and behavioural research and testing. To avoid unnecessary pain before , during and after experiment. To provide guidelines for : Housing , care , breeding and maintenance. Source of experimental animals. Acceptable experimental procedures for anesthesia and euthanasia.

5. FUNCTION Registration of establishments conducting animal experimentation or breeding of animals for this purpose. Selection and appointment of nominees in the Institutional Animal Ethics Committees(IAEC) of registered establishments. Approval of Animal House Facilities on the basis of reports of inspections conducted by CPCSEA. Permission for conducting experiments involving use of animals. Recommendation for import of animals for use in experiments. Actions against establishment in case of violation of any legal norm/stipulation.

6. VETERINARY CARE Adequate veterinary care must be provided and is the responsibility of a veterinarian or a person who has training or experience in laboratory animal science and medicine. Observed regularly for sign of illness , injury , or abnormal behaviour . Contagious disease – isolated from healthy animal.

7. ANIMAL PROCUREMENT All animals must be acquired lawfully as per the CPCSEA guidelines. A health surveillance program for screening incoming animals should be carried out to assess animal quality. Methods of transportation should also be taken into account. Each consignment of animals should be inspected for compliance with procurement specifications , and the animals should be quarantined and stabilized according to procedures appropriate for the species and circumstances.

8. QUARANTINE Separation of newly received animals from those already in the facility until the health and possibly the microbial status of newly received animal have been determine. A minimum duration of quarantine for small animal - 1 week and for larger animal - 6 week. An effective quarantine minimizes the chance for introduction of pathogens into an established colony.

9. STABILIZATION AND SEPARATION Stabilization :- Physiologic , psychological and nutritional stabilization should be given before their use. Duration of stabilization will depend on type and duration of animal transportation , and species of animal. Separation :- Physical separation of animal animals by species is recommended to prevent interspecies disease transmission and to eliminate anxiety and possible physiological and behavioural changes due to interspecies conflict. It shall be acceptable to house different species in the same room e.g. two species have a similar pathogen status and are behaviourally compatible.

10. SURVEILLANCE, DIAGNOSIS TREATMENT AND CONTROL DISEASE All animal should be observed for signs of illness, injury, or abnormal behaviour by animal house staff. Animals that show signs of a contagious disease should be isolated from healthy animals in the colony.

11. PERSONAL HYGIENE Animal care staff maintain a high standard of personal cleanliness. Clothing suitable for use in the animal facility. It acceptable to use disposable gear such as gloves, masks, head covers, coats, coveralls, and shoe covers. Person should change clothing as often as is necessary to maintain personal hygiene. Personnel should not be permitted to eat, drink, smoke or apply cosmetic in animal rooms.

12. TRANSPORT OF LABORATORY ANIMALS The main considerations for transport of animals are the mode of transport. The containers, the animal density in cages, food and water during transit. Protection from transit infection, injuries and stress.

13. PHYSICAL FACILITIES Building Material :- Moisture - proof, fire-resistant, seamless materials are most desirable for interior surfaces including vermin and pest resistance. Corridor :- Wide enough to facilitate the movement of personnel as well as equipment’s and should be kept clean. Utilities :- Water lines, drain pipes and electrical connection. Animal Room Doors :- Rust, vermin and dust proof. It properly within their frames and provided with an observation window. Floors :- Smooth, moisture proof, non-absorbent skid-proof.

14. ENVIRONMENT Air conditioning is for laboratory animals temperature within the range of 180-290 c relative humidity 30-70% throughout the year. For large animal comfortable zone 18-37c. POWE & LIGHTING The electrical system should be safe and provide appropriate lighting and a sufficient no. of power outlets. A time control light system should be used. NOISE CONTROL Noise free environment.

15. ANIMAL HUSBANDRY CAGING & HOUSING SYSTEM :- Adequate ventilation. Meet the biological need of animal. Keep the animal dry and clean. Cages made of stainless steel or painted steel. Feeding and watering devices should be easily accessible for filling, changing, cleaning and servicing.

16. FOOD :- Should be fed palatable, non-contaminated and nutritionally adequate food. Diet should be free from heavy metals. BEDDING :- Absorbent, free of toxic chemicals or other substances that could injure animals or personnel. Should be removed and replaced with fresh materials as often as necessary to keep animal clean and dry.

17. RECORD KEEPING Animal house plans, which includes typical floor plan, all fixtures etc. Animal house staff record – both technical and non-technical. Health record of staff. All SOPs relevant to the animals. Breeding, stock, purchase and sales records. Institutional Animal Ethics Committee meetings. Records of experiments conducted with the no. of animals used (copy of form B). Death Record.

18. ANAESTHESIA It must also be ensured that the anaesthesia is given for the full duration of experiments. And at no stage the animal is conscious to perceive pain during the experiment. Sedative, analgesics and anaesthetics should be used to control pain or distress under experiment.

19. EUTHANASIA The word “euthanasia” it comes from the Greek words “ eu ” (good) and “ thanatos ” (death). Purpose :- End of experiment, to provide tissue for scientific purpose. Free the animal from pain. Diseased animal or animal in bad condition.

20. METHODS Physical Methods :- Electrocution, Exsanguination , Decapitation, Cervical dislocation. Chemical Methods :- Drug administration overdose of choral hydrate, ketamine , chloroform.

21. Reference Icmr.nic.in/bioethics/final Cpcsea.nic.in Www.aaalac.org/resource/SOP Google

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