CPCSEA guidelines

Guidelines of CPCSEA

1. The Committee for the Purpose of Control and Supervision of Experiments on Animals (CPCSEA) Presented by- Smita kapoor

2. CPCSEA is a statutory Committee, established under Chapter 4, Section 15(1) of the Prevention of Cruelty to Animals Act 1960. India is one of the pioneering countries to institute Prevention of Cruelty to Animals Act in 1960. Detailed rules for experimentation on animals were first enacted by the Ministry of Agriculture in 1968. Present Committee was constituted on 30th August, 2012   At present, Shri Hem Pande , Additional Secretary, MoEF&CC is the Chairman of the Committee. INTRODUCTION

3. To promote the humane care of animals used in biomedical and behavioral research and testing. to provide specifications that will enhance animal well being. To provide quality in gaining advanced biological knowledge that is relevant to humans and animals

4. Guidelines VETERINARY CARE Adequate veterinary care must be provided and is the responsibility of a veterinarian. Daily observation . ANIMAL PROCUREMENT All animals must be acquired lawfully as per the CPCSEA guidelines . A health surveillance program for incoming animals should be carried out to assess animal quality. Inspect for compliance with procurement specifications.

5. QUARANTINE An effective quarantine minimizes the chance for introduction of pathogens into an established colony. A minimum duration of quarantine - Small lab animals - 1 week and - Larger animals - 6 weeks STABILIZATION AND SEPARATION Newly received animals should be given a period for physiologic , psychologic and nutritional stabilization before their use. Duration for stabilization will depend on the type of animal , transportation and intended use. Physical separation of animals by species is recommended .

6. SURVEILLANCE, DIAGNOSIS, TREATMENT AND CONTROL OF DISEASE Observe for signs of illness, injury, or abnormal behavior. Unexpected deaths and signs of illness should be reported . If animals are known to be exposed to an infectious agent the group should be kept intact and isolated during the process of diagnosis, treatment, and control. Diagnostic clinical laboratory may be made available.

7. ANIMAL CARE AND TECHNICAL PERSONNEL Employ people trained in laboratory animal science. They should be provide for both formal and on-the-job training. PERSONAL HYGIENE It is essential to maintain a high standard of personal cleanliness. Decontaminate clothing exposed to potentially hazardous microbial agents or toxic substances. Use disposable gear. No permission to eat, drink, smoke or apply cosmetics in animal rooms.

8. ANIMAL EXPERIMENTATION INVOLVING HAZARDOUS AGENTS Institutional Biosafety Committee. The procedures must be reviewed by both the Institutional Biosafety committee and Institutional Animal Ethics Committee (IAEC ). MULTIPLE SURGICAL PROCEDURES ON SINGLE ANIMAL Multiple surgical procedures not to be practiced unless specified in a protocol only approved by the IAEC. DURATIONS OF EXPERIMENTS No animal should be used for experimentation for more than 3 years unless adequate justification is provided.

9. PHYSICAL RESTRAINT Brief physical restraint can be accomplished manually or with devices. Prolonged restraint of any animal should be avoided unless essential to research objectives. Less restrictive systems, such as the tether system or the pole and collar system should be used when compatible with research objectives . The following are important guidelines for the use of restraint equipments : Not be used simply as a convenience in handling or managing animals. Minimum time. Should be given training to adapt to the equipment. Observe the animal at appropriate intervals. Veterinary care should be provided if lesions or illness associated with restraint are observed.

10. PHYSICAL PLANT The physical condition and design of animal facility hould be well planned and properly maintained. PHYSICAL RELATIONSHIP OF ANIMAL FACILITIES TO LABORATORY Isolated far away from human habitation. Place animal housing areas adjacent to or near laboratories but separated. FUNCTIONAL AREAS Sufficient animal area required to: Ensure separation of species or isolation of individual projects when necessary; Receive , quarantine, and isolate animals; Provide for animal housing.

11. PHYSICAL FACILITIES (1)Building material ( 2)Animal room doors (3)Floors (4)Drains (5)Storage areas (6)Experimental area (7)Corridor (8)Exterior windows ENVIRONMENT (a) TEMPERATURE AND HUMIDITY CONTROL 18 to 29°C temperature range3 % to70% relative humidity (b) VENTILATION

12. (c) POWER AND LIGHTING A time-controlled lighting system. Emergency power should be available in the event of power failure (d) NOISE CONTROL ANIMAL HUSBANDRY (a) CAGING OR HOUSING SYSTEM: Provide adequate space Comfortable environment Escape proof enclosure that confines animal safety. Easy access to food and water. Provide adequate ventilation. Meet the biological needs of the animals. Keep the animals dry and clean.

13. FOOD Animals should be fed palatable, non-contaminated, and nutritionally adequate food daily . BEDDING DESIRABLE CRITERIA Absorbent Free of toxic chemicals Ammonia binding Sterilizable Easily stored Uncontaminated Nontoxic Disposable by incineration Readily available

14. WATER Continuous access of fresh, potable, uncontaminated drinking water. Periodic monitoring of microbial contamination . SANITATION and CLEANLINESS Cleaning with detergents and disinfectants. Wire – bottom rodent cages should be washed at least every 2 weeks. Disinfection of cages by rinsing at a temperature of 82.20C (180 F ). WASTE DISPOSAL AND PEST CONTROL Remove regularly and frequently. Animal tissues, carcasses, and hazardous wastes should be lined with leak – proof, disposable liners. EMERGENCY , WEEKEND AND HOLIDAY CARE Animals should be cared by qualified personnel every day, including weekends and holidays.

15. RECORD KEEPING The Animal House should maintain following records: Animal House plans Animal House staff record All SOPs for animals Breeding , stock, purchase and sales records Minutes of institutional Animals Ethics Committee meetings Records of experiments Death Record Clinical record Water analysis report

16. STANDARD OPERATING PROCEDURES (SOPs) The Institute shall maintain SOPs describing procedures / methods of Animal Husbandry Maintenance Breeding Animal house microbial analysis Experimentation records . TRANSPORT OF LABORATORY ANIMALS The main considerations for transport of animals are: Mode of transport Containers Animal density in cages Food and water during transit Protection from transit infections Injuries and stress

17. OPERATIONAL GUIDELINES IN CPCSEA(10) Main activities (a) Registration of establishments for Breeding of animals: (b) Registration of establishments for experiments on animals : (c) Approval of Animal House Facilities: (d) Permission of Committee for Conducting Experiments: (e) Recommendation for import Regulatory Mechanism : Committee for the Purpose of Control and Supervision of Experiments on Animals (CPCSEA ) To ensure that animals are not subjected to unnecessary pain or suffering before, during or after performance of experiments on them. Sub-Committees of the CPCSEA: 1) Sub-Committee on Large Animals (SCLA). 2) Sub-Committee for selection of CPCSEA Nominees on Institutional Animals Ethics Committees.

18. Thank you