CPCSEA guidelines for laboratory animal facility.pptx

Anesthesia for laboratory animal

1. CPCSEA guidelines for laboratory animal facility : Anesthesia Presented by : Rushikesh Tidake M.Pharm Sem III Pharmacology RSCP , Buldana

2. Contents Introduction Need of anesthesia Types of anestheisa

3. CPCSEA Committee for the purpose of Control and Supervision of Experimental Animals Statutory body formed by Act of Indian Parliament under the prevention of Cruelty to Animals Act , 1960 Reviewed in 1998 , under the committed chairpersonship of Meneka Gandhi Head quarters at Chennai

4. Continue ... National level expert committee to scrutinize and approve the research projects conducted using animals Introduced a National “ Good Laboratory Practices” document Introduced the 3R’s for Replacement , Reduction and Refinement

5. Goals The goal of these guidelines is to promote the human care of animal used in biomedical and behavioural research and testing To avoid unnecessary pain before , during and after experiments To provide guideline for : Housing Care Breeding and Maintenance Source of experimental animals Acceptable experimental procedures for anesthesia and euthansia .

6. Functions Approval of Animal house Facility on the basis of inspection conducted by CPCSEA Recommendation for import of animals used in experiments Actions against in case of violation any legal norm . Permission for conducting experiments

7. Anesthesia Greek word an = without a sthesia = sensation It is the practice of administering medications that block the feeling of pain and other sensations that produce deep state of uncounsciousness that temporarily eliminate sensation & motor activity.

8. Need of anesthesia Ethical reason : for making the experiment or procedure to be painless. Practical reason : as “ chemical restraint ” for conducting the experiment or procedure smoothly and to avoid stress & trauma to the animal .

9. Pre anesthetic treatments Fasted for 8 hours All animals should have access to drinking water until 1 hour prior to induction of anesthesia Animals should be weighed to ensure accurate drug dosage calculations

10. Anesthetic agents Ideal anesthetic agents Should produce reversible & controlled loss of consciouness Should not disturb the physiological functions Causing relief from pain , stress & discomfort No mortality Easy to administered Should be economical

11. Types of anesthesia General anesthetics Inhalational agents : Isoflurane , sevoflurane , N2O Advantage : Rapid induction , recovery & precise dose titration Also useful for euthansia Rapid elimination of drug through lungs

12. Continue... Disadvantages : High cost & problem of using precision vaporizers Potential occupational exposure concern and the risk of fatal over dosage No residual analgesic activity Promotes progressive depression & hpoxia

13. Injectable anesthetic agents : Barbiturates : Thiopentone , Phenobarbitone Dissociatives : Ketamine Other : Urethane , Chloralose , Paraldehyde ,Magnesium Sulphate

14. Advantages : Do not require vapourizer & ventilation Require a low dose for induction Control of cardiopulmonary function is more adequate Disadvantages : Require repeated dosing Unpredictable depth of anesthesia Some agents may cause hypotension , hypertension