Creative Biolabs-CAMouse™-Fully Human Antibody Generation Platform

Creative Biolabs is highly specialized in therapeutic antibody discovery, and we have extensive experience in multifunctional antibody discovery methods.
This slide introduces CAMouse™, a new transgenic mouse strain from Creative Biolabs, and establishes a complete human antibody generation platform.

1. CAMouse ™ —— Fully Human Antibody Generation Platform www.creative-biolabs.com TEL: [phone] Email: [email protected]

2. Our Case Report Transgenic CAMouse ™ has robust immune responses and similar IgG titre as human. 2

3. Limitation of Therapeutic Antibody Development 3 Immunogenic side effects prevent improvement of most therapeutic antibodies. There are potential risks to the humanization approach.

4. CAMouse ™ Platform ——New Generation of Fully Human Antibody Production Creative Biolabs always pursue to provide the optimal humanized transgenic mouse strain. Now, we are proud to offer the unique and patented CAMouse ™ fully human antibody generation platform to researchers all over the world ”

5. Advantages of CAMouse ™ Platform Highly efficient gene introduction 100% human sequence Excellent immune system performance Skillful technique team Flexible licensing terms 5

6. CAMouse ™ VHH Strain Silence endogenou s m ouse Ig gene Introduce Ig gene Human heavy-chain-only antibodies Human IgH(VH,CH2-3) loci Mouse IgH chain Mouse Ig K chain 6

7. CAMouse ™ VHL Strain Silence endogenou s m ouse Ig gene Introduce Ig gene Fully huma n a ntibodies Human IgH (VH,CH2-3) loci Human IgL (Vk,CL) loci Mous e I gH chain Mous e I g K chain 7

8. CAMouse ™ VHK Strain Silence endogenou s m ouse Ig gene Introduce Ig gene Fully human antibodies Human IgH (VH,CH2-3) loci Human IgL (Vk,CL) loci Mous e I gH chain Mous e I g K chain 8

9. Immunized host animals Spleen B cell Cell culture Myeloma cells Antibody Development Technology-H ybridoma Screening Hybridoma mAb , Humanized mAb 9

10. Antibody Development Technology-P hage D isplay scFv/Fab/sdAb genes Screening Repeat screening Display PCR Premade library mAb, Human mAb, Humanized mAb Isolated B cell Surfa ce d isplay library 10 Cloning

11. Antibody Development Technology- B C ell S orting Specific B cell capture Screening Antibody genes cloning B cell Sorting RT-PCR Cloning Isolated B cells Antibody expression mAb, Human mAb with Na i ve-paired H- and L-chain Ag-specific B cell 11

12. Immunogen Design & Preparation Whole cells Peptide/Protein Small molecules Membrane proteins Our scientists have rich experience in dealing with various kinds of immunogens: small molecules, membrane proteins, cytokines, haptens , whole cells, etc. Upon custom requests, we can evaluate, design and prepare most appropriate immunogen in most appropriate and feasible format. 12

13. Application & Workflow Project Evaluation Antibody Delivery Immunization Scheme Immunogen Design & Preparation Antibody Discovery Campaign Function Validation Engineering & Optimization Antibody Production 13

14. Web: www.creative-biolabs.com Email: [email protected] Tel: [phone] Address: 45-1 Ramsey Road, Shirley, NY 11967, USA Fax: [phone]