Demonstration of viral inclusion bodies

Attuluri Vamsi Kumar, Assistant Professor, Division: MTL, SMAS, Galgotias University, UP.

1. Experiment No - 8 Demonstration of viral inclusion bodies Sub code : BMLT5051 Sub Name: Virology and mycology Practical Department: Department of MLT, SMAS Faculty: A. Vamsi Kumar Designation : Assistant professor Sem- V

2. SYLLABUS OVERVIEW

3. Unit – 3 Unit-3 Virology 7 hours Introduction, classification of virus, collection, Transport, & Storage of sample for Viral diagnosis. Staining Techniques used in virology. Processing of sample for viral diagnosis (Egg Inoculation & Tissue culture)

4. Experiment No - 8 Demonstration of Hematoxylin & Eosin stain

5. Aim To perform H&E stain for detection of inclusion bodies in the given sample.

6. Materials required H&E reagents Clean glass slide Viral culture Staining rack Distilled water Pipettes PPE’s

7. Procedure

8. Interpretation of results

9. https://www.barnsleyhospital.nhs.uk/pathology/microbiology/specimen-collection/skin-scrapes-hair-and-nail-samples-for-mycology/ https://dermnetnz.org/topics/laboratory-tests-for-fungal-infection/ https://bpac.org.nz/BT/2011/March/fungal-infections.aspx References