Determination of tartaric acid, phenolic and flavonoid content in Berberis integerrima Bunge fruit collected from Badakhshan province, Afghanistan. PPT.pptx

This PPT presents the standardization and determination of the amount of tartaric acid, phenolic and flavonoid content in barberry fruit collected from Badakhshan Province, Afghanistan.

1. Determination Of Phenolic, Flavonoid And Tartaric Acid Contents In Berberis integerrima Bunge Fruits Collected From Badakhshan Province, Afghanistan Najibullah Nowruzi, Rabia Ayoubi, Farida Shoaib Vedi, Salma Lutfi Nov / 2023 1 st Conference on Recent Pharmaceutical and Medical Research 1

2. Table of contents 01 Introduction 02 Objectives 03 Methodology 04 Results 05 Conclusion 06 References 2

3. Introduction 01 3

4. Introduction Barberry ( Berberis intergerrima Bunge) is one of the important medicinal, food and export plants of Afghanistan ( Rafiqpoor , et al., 2013). The fruit of this plant contains a large amount of organic acids, phenolic substances, flavonoids and vitamins and has a special place in the traditional medicine of the country ( Dehkordi , 2003). Several studies have proven the digestive, anti-inflammatory, antioxidant, anti-cancer and anti-microbial properties of barberry fruit (Moldovan, et al., 2005). Badakhshan province is one of the major barberry producing provinces in Afghanistan ( Rafiqpoor , et al., 2013 ). Berberis intergerrima Bunge Geo. Distribution of B. intergerrima in Afghanistan 4

5. C ontinue.. Standardization and determination of the bioactive phytoconstituents of the fruits collected from main resources of the plant will help to: Increase the quality of desired item Augment the acceptance rate as export good in national and international markets Optimization of processing condition Improvement of post harvesting treatment criteria But unfortunately, the harvested fruits in different p rovinces of our country not only does not get standardized but also illegally transported to neighboring nations and after some processing is sold as their exported items. So, in order to avoid the present scenario to certain content it is important to evaluate the quality of barberry fruits collected from its main resources in our country and optimize the harvesting process, if possible ( Baburi , 2016). 5

6. Objectives 02 6

7. Objectives To evaluate pharmacognostic characteristics of barberry fruits (BBFs22 ) To standardize the quality of BBFs22 To determine the TPC, TFC and TAC in BBFs22. 01 02 03

8. Methodology 03 8

9. Methodology . 9

10. Results 04 10

11. Results Pharmacognostic evaluation of barberry fruits (BBFs22) Organoleptic evaluation Table 1 Organoleptic and macroscopic characteristics of BBFs22. Organoleptic and Macroscopic Characteristics of BBFs22 Color Odor Taste Shape Size (mm) Presence of Seed Dark red berries Slightly aromatic Sour Ellipsoid 9 Large seed is present 11

12. C ontinue.. Pharmacognostic evaluation of barberry fruits (BBFs22) Macroscopic evaluation Figure 1 BBFs22 and its crushed form. 12

13. C ontinue.. Pharmacognostic evaluation of barberry fruits (BBFs22) Microscopic evaluation Figure 2 Transvers, longitudinal and surface sections of BBFs22. 1: Upper epidermis ( Exocarp ); 2: Seed; 3: Paranchymatous tissue. 1 2 3 13

14. C ontinue.. Pharmacognostic evaluation of barberry fruits (BBFs22) Preliminary Phytochemical Testing Table 2 Phytochemical studies of BBFs22.. No. Type of Secondary Metabolite Type of Extract Test Result 1 Flavonoids Methanolic Ammonia test Yellow color Shinoda test Light red color Vanillin HCL Light red color 2 Alkaloids Methanolic Dragendorff’s test orange-red precipitate 3 Tannins and Phenolics Aqueous FeCl 3 Bluish or black color Vanillin HCL Pink color 4 Saponins Aqueous Froth test Foam production Fontan_ Kendal Foam difference 5 Mucilage Aqueous Swelling index 0.16 14

15. C ontinue .. Standardization of BBFs22 Table 3 Various numerical values of BBFs22 obtained after standardization ... Numerical values (%) Test Sample Foreign matter Water soluble extractive Methanolic extractive Acid- insoluble ash Total ash Moisture content BBFs22 9.16±1.3 28.15± 0.19 23.77± 0.97 0.32± 0.05 2.11± 0.05 7.48± 0.13 n= 3, mean± SD 15

16. C ontinue.. Determination of: Total phenolic content (TPC) Total flavonoid content (TFC) Tartaric acid content (TAC) in BBFs22. Figure 3 Standard curve of gallic acid 16 ( Abs ) ( Conc .)

17. C ontinue.. Table 4 TPC, TFC, and TAC of BBFs22. Type of extract (B BFs22) TPC ( ) TFC ( ) TAC ( ) Aqueous 1.04 mg ± 22.29 3.03±0.38 mg 17.78±0.05 mg Methanolic 26.43±1.65 mg 3.10±0.04 mg 14.06±0.05 mg Type of extract (B BFs22) Aqueous 1.04 mg ± 22.29 3.03±0.38 mg 17.78±0.05 mg Methanolic 26.43±1.65 mg 3.10±0.04 mg 14.06±0.05 mg n= 3, mean± SD 17 (Abs ) (Abs ) ( Conc .)

18. Conclusion 05 18

19. Conclusion The investigation carried out to evaluate and determine the amount of BBFs22 compounds grown in Badakhshan province indicated that: BBFs22 had lesser ash content than the mentioned norms. BBFs22 , had lower moisture content than the available literature. The aqueous and methanolic extractive values of BBFs22 ​​were lower compared to the available standard values. Although in most of the cases the values obtained from the pharmacognostic evaluation of BBFs22 ​​were lower than the available data, but nevertheless, the mentioned values were within the standard range and this may consider as good quality of tested barberry fruits. D ifferent solvents can be used to determine the amount of phenolics , flavonoids and tartaric acid contests in the fruits. However, the results obtained from this study indicated that methanol is a better solvent than water for this purpose. 19

20. References S -W Breckle and M D Rafiqpoor . (2013) . vascular plants of afghanistan an Augmented checkist (6). ( A Dittmann (Edit, Bonn: The Deutsche Nationalbibiothek ). Cadmiel Moldovan, Oleg Frumuzachi , Mihai Babotă , Luigi Menghini , Stefania Cesa , Alexandru Gavan, . Lillian Barros. (2021) . Development of an Optimized Drying Process for the Recovery of Bioactive Compounds from the Autumn Fruits of Berberis vulgaris L. and Crataegus monogyna Jacq . MDPI , 2-4 . Nilda Ersoy , Muhammaed KUPE, Halil Ibarahim SAGBAS & Sezai ERCISLI. (2018) . Physicochemical Diversity Amonge Barbery ( Berberis vulgaris L.) Fruits from Eastern Anatolia. AcademicPres.42(2), 336-342. Mohammad Osman Baburi . (2016) . pharmacognosy of terpenoids and alkaloids (1). tehran : Samt . Nasrullah Ghasemi Dehkordi . (2003) . Iranin Herbal pharmacopoeia (2). Tehran: Minisrty of Heath, Medicine and Education Deputy Food and Drug . Behrad Z, Sefidkon F, Ghasemzadeh H, Rezadoos H, & Balandar A. (2021). Determination of Phenolic Compounds and Antioxidant Activities of 55 Iranian Berberis Genotypes. Journal of Medicinal Plants and By-products , 3-7 . F Sharifi and L Pourakbar . (2015) . Comparison of anti-oxidative properties of fresh Berberis (integerrima × Vulgaris) in Aqueous and alcoholic solvents. Iranian Food Science and Technology Research Journal,12(2), 296-304 . Muttalip Gundogdu . (2013) . Determination of Antioxidant Capacities and Biochemical Compounds of Berberis vulgaris L. Fruits. ResearchGate.7(2), 347-350. . 20

21. Thank You Any Question? 21